Monocyte Differentiation to Macrophage Requires Interferon Regulatory Factor 7

Runqing Lu, Paula M. Pitha

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Interferon regulatory factors are a growing family of transcription factor that have been implicated in cellular events such as cell-growth regulation, antiviral defense, and development of the immune system. Interferon regulatory factor 7 (IRF-7) is expressed predominantly in lymphoid tissues and has been studied extensively in the context of viral infection and the induction of interferon and cytokine gene expression. In this paper, the involvement of IRF-7 in monocyte differentiation was examined in U937, HL60, and human primary macrophages. We report the induction of IRF-7 expression by 12-O-tetradecanoylphorbol- 13-acetate in U937 and HL60 cells and demonstrate that this induction is essential for the monocyte differentiation to macrophages. We show that the monocyte differentiation is inhibited in cells expressing a dominant negative IRF-7 mutant, as evidenced by decreased expression of two macrophage-differentiation markers, CD11b and CD11c, and impaired phagocytic activity. In addition, we demonstrate that overexpression of IRF-7 is sufficient to trigger monocyte differentiation and to induce cell cycle arrest. The identification of IRF-7 as a key regulator in monocyte differentiation suggests a novel function of IRF-7 in innate immunity.

Original languageEnglish (US)
Pages (from-to)45491-45496
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number48
DOIs
StatePublished - Nov 30 2001

Fingerprint

Interferon Regulatory Factor-7
Macrophages
Monocytes
Interferon Regulatory Factors
Cells
U937 Cells
Immune system
HL-60 Cells
Differentiation Antigens
Cell growth
Lymphoid Tissue
Tetradecanoylphorbol Acetate
Virus Diseases
Cell Cycle Checkpoints
Innate Immunity
Gene expression
Interferons
Antiviral Agents
Immune System
Acetates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Monocyte Differentiation to Macrophage Requires Interferon Regulatory Factor 7. / Lu, Runqing; Pitha, Paula M.

In: Journal of Biological Chemistry, Vol. 276, No. 48, 30.11.2001, p. 45491-45496.

Research output: Contribution to journalArticle

Lu, Runqing ; Pitha, Paula M. / Monocyte Differentiation to Macrophage Requires Interferon Regulatory Factor 7. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 48. pp. 45491-45496.
@article{32597078e1b7437aabae7fe2b989d384,
title = "Monocyte Differentiation to Macrophage Requires Interferon Regulatory Factor 7",
abstract = "Interferon regulatory factors are a growing family of transcription factor that have been implicated in cellular events such as cell-growth regulation, antiviral defense, and development of the immune system. Interferon regulatory factor 7 (IRF-7) is expressed predominantly in lymphoid tissues and has been studied extensively in the context of viral infection and the induction of interferon and cytokine gene expression. In this paper, the involvement of IRF-7 in monocyte differentiation was examined in U937, HL60, and human primary macrophages. We report the induction of IRF-7 expression by 12-O-tetradecanoylphorbol- 13-acetate in U937 and HL60 cells and demonstrate that this induction is essential for the monocyte differentiation to macrophages. We show that the monocyte differentiation is inhibited in cells expressing a dominant negative IRF-7 mutant, as evidenced by decreased expression of two macrophage-differentiation markers, CD11b and CD11c, and impaired phagocytic activity. In addition, we demonstrate that overexpression of IRF-7 is sufficient to trigger monocyte differentiation and to induce cell cycle arrest. The identification of IRF-7 as a key regulator in monocyte differentiation suggests a novel function of IRF-7 in innate immunity.",
author = "Runqing Lu and Pitha, {Paula M.}",
year = "2001",
month = "11",
day = "30",
doi = "10.1074/jbc.C100421200",
language = "English (US)",
volume = "276",
pages = "45491--45496",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "48",

}

TY - JOUR

T1 - Monocyte Differentiation to Macrophage Requires Interferon Regulatory Factor 7

AU - Lu, Runqing

AU - Pitha, Paula M.

PY - 2001/11/30

Y1 - 2001/11/30

N2 - Interferon regulatory factors are a growing family of transcription factor that have been implicated in cellular events such as cell-growth regulation, antiviral defense, and development of the immune system. Interferon regulatory factor 7 (IRF-7) is expressed predominantly in lymphoid tissues and has been studied extensively in the context of viral infection and the induction of interferon and cytokine gene expression. In this paper, the involvement of IRF-7 in monocyte differentiation was examined in U937, HL60, and human primary macrophages. We report the induction of IRF-7 expression by 12-O-tetradecanoylphorbol- 13-acetate in U937 and HL60 cells and demonstrate that this induction is essential for the monocyte differentiation to macrophages. We show that the monocyte differentiation is inhibited in cells expressing a dominant negative IRF-7 mutant, as evidenced by decreased expression of two macrophage-differentiation markers, CD11b and CD11c, and impaired phagocytic activity. In addition, we demonstrate that overexpression of IRF-7 is sufficient to trigger monocyte differentiation and to induce cell cycle arrest. The identification of IRF-7 as a key regulator in monocyte differentiation suggests a novel function of IRF-7 in innate immunity.

AB - Interferon regulatory factors are a growing family of transcription factor that have been implicated in cellular events such as cell-growth regulation, antiviral defense, and development of the immune system. Interferon regulatory factor 7 (IRF-7) is expressed predominantly in lymphoid tissues and has been studied extensively in the context of viral infection and the induction of interferon and cytokine gene expression. In this paper, the involvement of IRF-7 in monocyte differentiation was examined in U937, HL60, and human primary macrophages. We report the induction of IRF-7 expression by 12-O-tetradecanoylphorbol- 13-acetate in U937 and HL60 cells and demonstrate that this induction is essential for the monocyte differentiation to macrophages. We show that the monocyte differentiation is inhibited in cells expressing a dominant negative IRF-7 mutant, as evidenced by decreased expression of two macrophage-differentiation markers, CD11b and CD11c, and impaired phagocytic activity. In addition, we demonstrate that overexpression of IRF-7 is sufficient to trigger monocyte differentiation and to induce cell cycle arrest. The identification of IRF-7 as a key regulator in monocyte differentiation suggests a novel function of IRF-7 in innate immunity.

UR - http://www.scopus.com/inward/record.url?scp=0035977009&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035977009&partnerID=8YFLogxK

U2 - 10.1074/jbc.C100421200

DO - 10.1074/jbc.C100421200

M3 - Article

C2 - 11585813

AN - SCOPUS:0035977009

VL - 276

SP - 45491

EP - 45496

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 48

ER -