Monocyte 5-HT1A receptors mediate pindobind suppression of natural killer cell activity: Modulation by catalase

M. G. Frank, D. R. Johnson, S. E. Hendricks, J. L. Wieseler Frank

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Serotonin (5-hydroxytryptamine; 5-HT) modulates constituents of the immune system. 5-HT1A receptor antagonists potently suppress lymphocyte function. NK cell activity (NKCA) was measured after exposure of mononuclear cells to the 5-HT1A receptor antagonist pindobind and the 5-HT1C/2 receptor antagonist ketanserin. Elutriated monocytes were exposed to pindobind, incubated with peripheral blood lymphocytes (PBL) in the presence or absence of an H2O2 scavenger catalase, and NKCA measured. Pindobind, but not ketanserin, suppressed NKCA in vitro. Pindobind-treated monocytes suppressed NKCA, whereas pindobind treatment of PBL did not affect NKCA. Catalase inhibited pindobind-induced suppression of NKCA. These data are consistent with previous results that 5-HT modulates NKCA via 5-HT1A receptors on monocytes and suggest that 5-HT may abrogate monocyte suppression of NKCA by inhibiting monocyte signals such as H2O2.

Original languageEnglish (US)
Pages (from-to)247-253
Number of pages7
JournalInternational Immunopharmacology
Volume1
Issue number2
DOIs
Publication statusPublished - Mar 12 2001

    Fingerprint

Keywords

  • 5-HT receptors
  • HO
  • Monocytes
  • NK cells
  • Serotonin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this