Monoclonal Antibody against Mouse CAR following Genetic Immunization

Steven D. Carson, Barbara L. Switzer, Steven M. Tracy, Nora M. Chapman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

To broaden our repertoire of monoclonal antibodies against CAR (coxsackievirus and adenovirus receptor), we inoculated mice with an expression vector containing the cDNA encoding human CAR extracellular and transmembrane sequence, and boosted the response by inoculation with soluble human CAR protein produced in E. coli. Of the hybridomas obtained following this immunization protocol, one secreted IgG with exceptional reactivity against mouse CAR. Since CAR has been shown to form dimers, expression of human CAR in cells that express mouse CAR may have stimulated the host immune system to recognize endogenous CAR in heterodimers.

Original languageEnglish (US)
Pages (from-to)19-22
Number of pages4
JournalHybridoma and Hybridomics
Volume23
Issue number1
DOIs
StatePublished - Feb 1 2004

Fingerprint

Enterovirus
Immunization
Human Adenoviruses
Monoclonal Antibodies
Hybridomas
Immune System
Complementary DNA
Immunoglobulin G
mouse CLMP protein
adenovirus receptor
Escherichia coli
Proteins

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Monoclonal Antibody against Mouse CAR following Genetic Immunization. / Carson, Steven D.; Switzer, Barbara L.; Tracy, Steven M.; Chapman, Nora M.

In: Hybridoma and Hybridomics, Vol. 23, No. 1, 01.02.2004, p. 19-22.

Research output: Contribution to journalArticle

Carson, Steven D. ; Switzer, Barbara L. ; Tracy, Steven M. ; Chapman, Nora M. / Monoclonal Antibody against Mouse CAR following Genetic Immunization. In: Hybridoma and Hybridomics. 2004 ; Vol. 23, No. 1. pp. 19-22.
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