Monoclonal antibodies with defined specificities for Torpedo nicotinic acetylcholine receptor cross-react with Drosophila neural tissue

B. A. Chase, J. Holliday, J. H. Reese, L. L.Y. Chun, E. Hawrot

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A panel of monoclonal antibodies with known specificity for the well-characterized nicotinic acetylcholine receptor from the electroplax of Torpedo californica, many of which cross-react with the mammalian muscle acetylcholine receptor, were examined for cross-reactivity in the fly, Drosophila melanogaster. Monoclonal antibodies with specificities for different epitopes on the transmembrane receptor complex from Torpedo cross-react with different regional subsets of neural tissue in Drosophila. Axonal tracts, neuropil, mechano-sensory bristle elements and photoreceptors, each are detected by separate monoclonal antibody classes corresponding to different epitope domains. A preliminary characterization of an antigenic determinant in Drosophila heads recognized by one of the cross-reacting monoclonal antibodies is presented. Monoclonal antibodies such as these may be useful in identifying molecules of homologous structure or function, possibly including a neuronal acetylcholine receptor.

Original languageEnglish (US)
Pages (from-to)959-976
Number of pages18
JournalNeuroscience
Volume21
Issue number3
DOIs
StatePublished - Jun 1987

Fingerprint

Torpedo
Nicotinic Receptors
Drosophila
Monoclonal Antibodies
Epitopes
Cholinergic Receptors
Neuropil
Antibody Specificity
Immunoglobulin Isotypes
Drosophila melanogaster
Diptera
Head
Muscles

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Monoclonal antibodies with defined specificities for Torpedo nicotinic acetylcholine receptor cross-react with Drosophila neural tissue. / Chase, B. A.; Holliday, J.; Reese, J. H.; Chun, L. L.Y.; Hawrot, E.

In: Neuroscience, Vol. 21, No. 3, 06.1987, p. 959-976.

Research output: Contribution to journalArticle

@article{1460e79603a042d1913eccff0207a218,
title = "Monoclonal antibodies with defined specificities for Torpedo nicotinic acetylcholine receptor cross-react with Drosophila neural tissue",
abstract = "A panel of monoclonal antibodies with known specificity for the well-characterized nicotinic acetylcholine receptor from the electroplax of Torpedo californica, many of which cross-react with the mammalian muscle acetylcholine receptor, were examined for cross-reactivity in the fly, Drosophila melanogaster. Monoclonal antibodies with specificities for different epitopes on the transmembrane receptor complex from Torpedo cross-react with different regional subsets of neural tissue in Drosophila. Axonal tracts, neuropil, mechano-sensory bristle elements and photoreceptors, each are detected by separate monoclonal antibody classes corresponding to different epitope domains. A preliminary characterization of an antigenic determinant in Drosophila heads recognized by one of the cross-reacting monoclonal antibodies is presented. Monoclonal antibodies such as these may be useful in identifying molecules of homologous structure or function, possibly including a neuronal acetylcholine receptor.",
author = "Chase, {B. A.} and J. Holliday and Reese, {J. H.} and Chun, {L. L.Y.} and E. Hawrot",
year = "1987",
month = "6",
doi = "10.1016/0306-4522(87)90051-0",
language = "English (US)",
volume = "21",
pages = "959--976",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Monoclonal antibodies with defined specificities for Torpedo nicotinic acetylcholine receptor cross-react with Drosophila neural tissue

AU - Chase, B. A.

AU - Holliday, J.

AU - Reese, J. H.

AU - Chun, L. L.Y.

AU - Hawrot, E.

PY - 1987/6

Y1 - 1987/6

N2 - A panel of monoclonal antibodies with known specificity for the well-characterized nicotinic acetylcholine receptor from the electroplax of Torpedo californica, many of which cross-react with the mammalian muscle acetylcholine receptor, were examined for cross-reactivity in the fly, Drosophila melanogaster. Monoclonal antibodies with specificities for different epitopes on the transmembrane receptor complex from Torpedo cross-react with different regional subsets of neural tissue in Drosophila. Axonal tracts, neuropil, mechano-sensory bristle elements and photoreceptors, each are detected by separate monoclonal antibody classes corresponding to different epitope domains. A preliminary characterization of an antigenic determinant in Drosophila heads recognized by one of the cross-reacting monoclonal antibodies is presented. Monoclonal antibodies such as these may be useful in identifying molecules of homologous structure or function, possibly including a neuronal acetylcholine receptor.

AB - A panel of monoclonal antibodies with known specificity for the well-characterized nicotinic acetylcholine receptor from the electroplax of Torpedo californica, many of which cross-react with the mammalian muscle acetylcholine receptor, were examined for cross-reactivity in the fly, Drosophila melanogaster. Monoclonal antibodies with specificities for different epitopes on the transmembrane receptor complex from Torpedo cross-react with different regional subsets of neural tissue in Drosophila. Axonal tracts, neuropil, mechano-sensory bristle elements and photoreceptors, each are detected by separate monoclonal antibody classes corresponding to different epitope domains. A preliminary characterization of an antigenic determinant in Drosophila heads recognized by one of the cross-reacting monoclonal antibodies is presented. Monoclonal antibodies such as these may be useful in identifying molecules of homologous structure or function, possibly including a neuronal acetylcholine receptor.

UR - http://www.scopus.com/inward/record.url?scp=0023193680&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023193680&partnerID=8YFLogxK

U2 - 10.1016/0306-4522(87)90051-0

DO - 10.1016/0306-4522(87)90051-0

M3 - Article

C2 - 2442658

AN - SCOPUS:0023193680

VL - 21

SP - 959

EP - 976

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 3

ER -