Monoclonal antibodies to the α- and β-subunits of the plant mitochondrial F1-ATPase

Michael H. Luethy, Arnost Horak, Thomas E. Elthon

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We have generated nine monoclonal antibodies against subunits of the maize (Zea mays L.) mitochondrial F1-ATPase. These monoclonal antibodies were generated by immunizing mice against maize mitochondrial fractions and randomly collecting useful hybridomas. To prove that these monoclonal antibodies were directed against ATPase subunits, we tested their cross-reactivity with purified F1-ATPase from pea cotyledon mitochondria. One of the antibodies (α-ATPaseD) cross-reacted with the pea F1-ATPase α-subunit and two (β-ATPaseD and β-ATPaseE) cross-reacted with the pea F1-ATPase β-subunit. This established that, of the nine antibodies, four react with the maize α-ATPase subunit and the other five react with the maize β-ATPase subunit. Most of the monoclonal antibodies cross-react with the F1-ATPase from a wide range of plant species. Each of the four monoclonal antibodies raised against the α-subunit recognizes a different epitope. Of the five β-subunit antibodies, at least three different epitopes are recognized. Direct incubation of the monoclonal antibodies with the F1-ATPase failed to inhibit the ATPase activity. The monoclonal antibodies α-ATPaseD and β-ATPaseD were bound to epoxide-glass QuantAffinity beads and incubated with a purified preparation of pea F1-ATPase. The ATPase activity was not inhibited when the antibodies bound the ATPase. The antibodies were used to help map the pea F1-ATPase subunits on a two-dimensional map of whole pea cotyledon mitochondrial protein. In addition, the antibodies have revealed antigenic similarities between various isoforms observed for the α- and β-subunits of the purified F1-ATPase. The specificity of these monoclonal antibodies, along with their cross-species recognition and their ability to bind the F1-ATPase without inhibiting enzymic function, makes these antibodies useful and invaluable tools for the further purification and characterization of plant mitochondrial F1-ATPases.

Original languageEnglish (US)
Pages (from-to)931-937
Number of pages7
JournalPlant physiology
Volume101
Issue number3
DOIs
StatePublished - Mar 1993

Fingerprint

H-transporting ATP synthase
Proton-Translocating ATPases
monoclonal antibodies
Monoclonal Antibodies
Peas
adenosinetriphosphatase
Adenosine Triphosphatases
peas
Zea mays
antibodies
Antibodies
corn
Cotyledon
epitopes
cotyledons
Epitopes
hybridomas
Mitochondrial Proteins
Epoxy Compounds
Hybridomas

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Monoclonal antibodies to the α- and β-subunits of the plant mitochondrial F1-ATPase. / Luethy, Michael H.; Horak, Arnost; Elthon, Thomas E.

In: Plant physiology, Vol. 101, No. 3, 03.1993, p. 931-937.

Research output: Contribution to journalArticle

Luethy, Michael H. ; Horak, Arnost ; Elthon, Thomas E. / Monoclonal antibodies to the α- and β-subunits of the plant mitochondrial F1-ATPase. In: Plant physiology. 1993 ; Vol. 101, No. 3. pp. 931-937.
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