Monoclonal antibodies to glutathione S‐Transferase π–immunohistochemical analysis of human tissues and cancers

Raphe R.S. Kantor, Steven L. Giardina, Armando Bartolazzi, Allen J. Townsend, Charles E. Myers, Kenneth H. Cowan, Dan L. Longo, Pier G. Natali

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Mouse monoclonal antibodies (MAb) have been generated against the anionic isozyme of human glutathione S‐transferase (GST π). MAb AGST I can inhibit 50‐70% of GST π enzymatic activity and reacts with a 3‐dimensional epitope which includes a putative glutathione binding site on GST π. A sandwich enzyme‐immunoassay established using MAb AGST I and a polyclonal antibody displayed a sensitivity of 0.5 ng/ml. Immunohistochemical analysis of human tissues demonstrated marked increases in GST π levels in cancers of the brain, cervix, endometrium, colon, rectum and testis and in fibro‐and chondrosarcomas.

Original languageEnglish (US)
Pages (from-to)193-201
Number of pages9
JournalInternational Journal of Cancer
Volume47
Issue number2
DOIs
StatePublished - Jan 21 1991

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Glutathione
Monoclonal Antibodies
Neoplasms
Chondrosarcoma
Endometrium
Rectum
Brain Neoplasms
Uterine Cervical Neoplasms
Isoenzymes
Testis
Epitopes
Colon
Binding Sites
Antibodies

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Monoclonal antibodies to glutathione S‐Transferase π–immunohistochemical analysis of human tissues and cancers. / Kantor, Raphe R.S.; Giardina, Steven L.; Bartolazzi, Armando; Townsend, Allen J.; Myers, Charles E.; Cowan, Kenneth H.; Longo, Dan L.; Natali, Pier G.

In: International Journal of Cancer, Vol. 47, No. 2, 21.01.1991, p. 193-201.

Research output: Contribution to journalArticle

Kantor, RRS, Giardina, SL, Bartolazzi, A, Townsend, AJ, Myers, CE, Cowan, KH, Longo, DL & Natali, PG 1991, 'Monoclonal antibodies to glutathione S‐Transferase π–immunohistochemical analysis of human tissues and cancers', International Journal of Cancer, vol. 47, no. 2, pp. 193-201. https://doi.org/10.1002/ijc.2910470206
Kantor, Raphe R.S. ; Giardina, Steven L. ; Bartolazzi, Armando ; Townsend, Allen J. ; Myers, Charles E. ; Cowan, Kenneth H. ; Longo, Dan L. ; Natali, Pier G. / Monoclonal antibodies to glutathione S‐Transferase π–immunohistochemical analysis of human tissues and cancers. In: International Journal of Cancer. 1991 ; Vol. 47, No. 2. pp. 193-201.
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