Monoclonal antibodies to glutathione S‐Transferase π–immunohistochemical analysis of human tissues and cancers

Raphe R.S. Kantor, Steven L. Giardina, Armando Bartolazzi, Allen J. Townsend, Charles E. Myers, Kenneth H Cowan, Dan L. Longo, Pier G. Natali

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Mouse monoclonal antibodies (MAb) have been generated against the anionic isozyme of human glutathione S‐transferase (GST π). MAb AGST I can inhibit 50‐70% of GST π enzymatic activity and reacts with a 3‐dimensional epitope which includes a putative glutathione binding site on GST π. A sandwich enzyme‐immunoassay established using MAb AGST I and a polyclonal antibody displayed a sensitivity of 0.5 ng/ml. Immunohistochemical analysis of human tissues demonstrated marked increases in GST π levels in cancers of the brain, cervix, endometrium, colon, rectum and testis and in fibro‐and chondrosarcomas.

Original languageEnglish (US)
Pages (from-to)193-201
Number of pages9
JournalInternational Journal of Cancer
Volume47
Issue number2
DOIs
StatePublished - Jan 1 1991

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Glutathione
Monoclonal Antibodies
Neoplasms
Chondrosarcoma
Endometrium
Rectum
Brain Neoplasms
Uterine Cervical Neoplasms
Isoenzymes
Testis
Epitopes
Colon
Binding Sites
Antibodies

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Monoclonal antibodies to glutathione S‐Transferase π–immunohistochemical analysis of human tissues and cancers. / Kantor, Raphe R.S.; Giardina, Steven L.; Bartolazzi, Armando; Townsend, Allen J.; Myers, Charles E.; Cowan, Kenneth H; Longo, Dan L.; Natali, Pier G.

In: International Journal of Cancer, Vol. 47, No. 2, 01.01.1991, p. 193-201.

Research output: Contribution to journalArticle

Kantor, RRS, Giardina, SL, Bartolazzi, A, Townsend, AJ, Myers, CE, Cowan, KH, Longo, DL & Natali, PG 1991, 'Monoclonal antibodies to glutathione S‐Transferase π–immunohistochemical analysis of human tissues and cancers', International Journal of Cancer, vol. 47, no. 2, pp. 193-201. https://doi.org/10.1002/ijc.2910470206
Kantor, Raphe R.S. ; Giardina, Steven L. ; Bartolazzi, Armando ; Townsend, Allen J. ; Myers, Charles E. ; Cowan, Kenneth H ; Longo, Dan L. ; Natali, Pier G. / Monoclonal antibodies to glutathione S‐Transferase π–immunohistochemical analysis of human tissues and cancers. In: International Journal of Cancer. 1991 ; Vol. 47, No. 2. pp. 193-201.
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