Monoclonal antibodies to distinct regions of human myelin proteolipid protein simultaneously recognize central nervous system myelin and neurons of many vertebrate species

Edward A. Greenfield, N R Jayagopala Reddy, Andrew Lees, Charissa A. Dyer, Omanand Koul, Khuong Nguyen, Shannon Bell, Nasim Kassam, Julian Hinojoza, Mary Jane Eaton, Marjorie B. Lees, Vijay K. Kuchroo, Raymond A. Sobel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Myelin proteolipid protein (PLP), the major protein of mammalian CNS myelin, is a member of the proteolipid gene family (pgf). It is an evolutionary conserved polytopic integral membrane protein and a potential autoantigen in multiple sclerosis (MS). To analyze antibody recognition of PLP epitopes in situ, monoclonal antibodies (mAbs) specific for different regions of human PLP (50-69, 100-123, 139-151, 178-191, 200-219, 264-276) were generated and used to immunostain CNS tissues of representative vertebrates. mAbs to each region recognized whole human PLP on Western blots; the anti-100-123 mAb did not recognize DM-20, the PLP isoform that lacks residues 116-150. All of the mAbs stained fixed, permeabilized oligodendrocytes and mammalian and avian CNS tissue myelin. Most of the mAbs also stained amphibian, teleost, and elasmobranch CNS myelin despite greater diversity of their pgf myelin protein sequences. Myelin staining was observed when there was at least 40% identity of the mAb epitope and known pgf myelin proteins of the same or related species. The pgf myelin proteins of teleosts and elasmobranchs lack 116-150; the anti-100-123 mAb did not stain their myelin. In addition to myelin, the anti-178-191 mAb stained many neurons in all species; other mAbs stained distinct neuron subpopulations in different species. Neuronal staining was observed when there was at least approximately 30% identity of the PLP mAb epitope and known pgf neuronal proteins of the same or related species. Thus, anti-human PLP epitope mAbs simultaneously recognize CNS myelin and neurons even without extensive sequence identity. Widespread anti-PLP mAb recognition of neurons suggests a novel potential pathophysiologic mechanism in MS patients, i.e., that anti-PLP antibodies associated with demyelination might simultaneously recognize pgf epitopes in neurons, thereby affecting their functions.

Original languageEnglish (US)
Pages (from-to)415-431
Number of pages17
JournalJournal of Neuroscience Research
Volume83
Issue number3
DOIs
StatePublished - Feb 15 2006

Fingerprint

Myelin Proteolipid Protein
Proteolipids
Myelin Sheath
Vertebrates
Central Nervous System
Monoclonal Antibodies
Neurons
Epitopes
Myelin Proteins
Proteins
Elasmobranchii
Genes
Multiple Sclerosis
Staining and Labeling
Antibodies
Oligodendroglia
Autoantigens

Keywords

  • Demyelination
  • Evolution
  • M6 proteins
  • Multiple sclerosis
  • Rhombex

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Monoclonal antibodies to distinct regions of human myelin proteolipid protein simultaneously recognize central nervous system myelin and neurons of many vertebrate species. / Greenfield, Edward A.; Reddy, N R Jayagopala; Lees, Andrew; Dyer, Charissa A.; Koul, Omanand; Nguyen, Khuong; Bell, Shannon; Kassam, Nasim; Hinojoza, Julian; Eaton, Mary Jane; Lees, Marjorie B.; Kuchroo, Vijay K.; Sobel, Raymond A.

In: Journal of Neuroscience Research, Vol. 83, No. 3, 15.02.2006, p. 415-431.

Research output: Contribution to journalArticle

Greenfield, EA, Reddy, NRJ, Lees, A, Dyer, CA, Koul, O, Nguyen, K, Bell, S, Kassam, N, Hinojoza, J, Eaton, MJ, Lees, MB, Kuchroo, VK & Sobel, RA 2006, 'Monoclonal antibodies to distinct regions of human myelin proteolipid protein simultaneously recognize central nervous system myelin and neurons of many vertebrate species', Journal of Neuroscience Research, vol. 83, no. 3, pp. 415-431. https://doi.org/10.1002/jnr.20748
Greenfield, Edward A. ; Reddy, N R Jayagopala ; Lees, Andrew ; Dyer, Charissa A. ; Koul, Omanand ; Nguyen, Khuong ; Bell, Shannon ; Kassam, Nasim ; Hinojoza, Julian ; Eaton, Mary Jane ; Lees, Marjorie B. ; Kuchroo, Vijay K. ; Sobel, Raymond A. / Monoclonal antibodies to distinct regions of human myelin proteolipid protein simultaneously recognize central nervous system myelin and neurons of many vertebrate species. In: Journal of Neuroscience Research. 2006 ; Vol. 83, No. 3. pp. 415-431.
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abstract = "Myelin proteolipid protein (PLP), the major protein of mammalian CNS myelin, is a member of the proteolipid gene family (pgf). It is an evolutionary conserved polytopic integral membrane protein and a potential autoantigen in multiple sclerosis (MS). To analyze antibody recognition of PLP epitopes in situ, monoclonal antibodies (mAbs) specific for different regions of human PLP (50-69, 100-123, 139-151, 178-191, 200-219, 264-276) were generated and used to immunostain CNS tissues of representative vertebrates. mAbs to each region recognized whole human PLP on Western blots; the anti-100-123 mAb did not recognize DM-20, the PLP isoform that lacks residues 116-150. All of the mAbs stained fixed, permeabilized oligodendrocytes and mammalian and avian CNS tissue myelin. Most of the mAbs also stained amphibian, teleost, and elasmobranch CNS myelin despite greater diversity of their pgf myelin protein sequences. Myelin staining was observed when there was at least 40{\%} identity of the mAb epitope and known pgf myelin proteins of the same or related species. The pgf myelin proteins of teleosts and elasmobranchs lack 116-150; the anti-100-123 mAb did not stain their myelin. In addition to myelin, the anti-178-191 mAb stained many neurons in all species; other mAbs stained distinct neuron subpopulations in different species. Neuronal staining was observed when there was at least approximately 30{\%} identity of the PLP mAb epitope and known pgf neuronal proteins of the same or related species. Thus, anti-human PLP epitope mAbs simultaneously recognize CNS myelin and neurons even without extensive sequence identity. Widespread anti-PLP mAb recognition of neurons suggests a novel potential pathophysiologic mechanism in MS patients, i.e., that anti-PLP antibodies associated with demyelination might simultaneously recognize pgf epitopes in neurons, thereby affecting their functions.",
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AU - Bell, Shannon

AU - Kassam, Nasim

AU - Hinojoza, Julian

AU - Eaton, Mary Jane

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