Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species

Charles N. Pegram, Lawrence F. Eng, Carol J. Wikstrand, Rodney D McComb, Yueng Ling Lee, Darell D. Bigner

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The highly reproducible histochemical localization of glial fibrillary acidic protein (GFAP)‡ qualifies it as an important marker of astrocytes in both research and clinical applications. The primary objective of this study was to produce monoclonal antibodies having the advantage of invariant specificity, affinity, and titer to GFAP-specific epitopes of wide species distribution. We report here the characterization of four monoclonal antibodies that recognize the same or spatially close epitopes specific to GFAP. The epitope(s) detected has been phylogenetically conserved; human, bovine, ovine, canine, porcine, rabbit, guinea pig, rat, murine, and chicken brain homogenates all specifically absorb monoclonal antibody activity. Of importance to the routine application of these new anti-GFAP monoclonal antibodies is the demonstration here of the stability of the antigen-antibody interaction in normal, reactive, and neoplastic astrocytes of both rat and human origing following various methods of fixation.

Original languageEnglish (US)
Pages (from-to)119-138
Number of pages20
JournalNeurochemical Pathology
Volume3
Issue number2
DOIs
Publication statusPublished - Jun 1 1983

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Keywords

  • Glial fibrillary acidic protein, monoclonal antibodies to and interspecies of
  • antibodies, monoclonal, of GFAP
  • astrocytes, monoclonal antibodies to GFAP of
  • intermediate filament proteins, glial fibrillary acidic protein-specific monoclonal antibodies
  • monoclonal antibodies, definition of specific interspecies GFAP epitopes with

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Neurology

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