Abstract

The authors investigated the ability of 70 monoclonal antibodies obtained from the Third International Workshop on Human Leukocyte Antigens (Oxford, 1986) to mark T lymphocytes in B5-fixed paraffin-embedded tissue. No staining occurred with 65 of the antibodies; however, 5 antibodies marked small lymphocytes in the T-cell areas of human tonsil. Two antibodies which strongly labeled lymphocytes, UCHL1 and T2/48, were used to examine 106 cases of non-Hodgkin's lymphoma, 29 cases of Hodgkin's disease, and a variety of normal and neoplastic tissues. UCHL1 and T2/48 each marked 86% (37/43) of B5-fixed T-cell lymphomas. Only 50% of formalin-fixed T-cell lymphomas were marked with these antibodies. UCHL1 marked 1.8% (1/56) of the B-cell lymphomas, compared with T2/48, which marked 19.6% (11/56) of the B-cell lymphomas. T2/48 had the interesting attribute of marking cells of the follicular mantle-zone and intermediate lymphocytic lymphoma, suggesting that the antibody recognizes a B-cell differentiation antigen. No Reed-Sternberg cells, epithelial neoplasms, sarcomas, neurogenic tumors, or normal nonlymphoid tissue were marked by either antibody. These antibodies successfully mark T cells in paraffin tissue sections and should aid in the investigation and characterization of abnormal lymphoid proliferations, 'undifferentiated' malignant neoplasms, and immunologically mediated disorders.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalAmerican Journal of Pathology
Volume127
Issue number1
StatePublished - Jun 23 1987

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Paraffin
Monoclonal Antibodies
T-Lymphocytes
Antibodies
T-Cell Lymphoma
B-Cell Lymphoma
B-Lymphocyte Differentiation Antigens
Neurofibrosarcoma
Lymphocytes
Reed-Sternberg Cells
Mantle-Cell Lymphoma
Glandular and Epithelial Neoplasms
Palatine Tonsil
HLA Antigens
Hodgkin Disease
Non-Hodgkin's Lymphoma
Formaldehyde
Neoplasms
Staining and Labeling
Education

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Monoclonal antibodies marking T lymphocytes in paraffin-embedded tissue. / Linder, James; Ye, Y.; Harrington, D. S.; Armitage, James Olen; Weisenburger, D. D.

In: American Journal of Pathology, Vol. 127, No. 1, 23.06.1987, p. 1-8.

Research output: Contribution to journalArticle

Linder, James ; Ye, Y. ; Harrington, D. S. ; Armitage, James Olen ; Weisenburger, D. D. / Monoclonal antibodies marking T lymphocytes in paraffin-embedded tissue. In: American Journal of Pathology. 1987 ; Vol. 127, No. 1. pp. 1-8.
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