Monoclonal antibodies against xenopus greatwall kinase

Ling Wang, Laura A. Fisher, James K Wahl, Aimin Peng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mitosis is known to be regulated by protein kinases, including MPF, Plk1, Aurora kinases, and so on, which become active in M-phase and phosphorylate a wide range of substrates to control multiple aspects of mitotic entry, progression, and exit. Mechanistic investigations of these kinases not only provide key insights into cell cycle regulation, but also hold great promise for cancer therapy. Recent studies, largely in Xenopus, characterized a new mitotic kinase named Greatwall (Gwl) that plays essential roles in both mitotic entry and maintenance. In this study, we generated a panel of mouse monoclonal antibodies (MAbs) specific for Xenopus Gwl and characterized these antibodies for their utility in immunoblotting, immunoprecipitation, and immunodepletion in Xenopus egg extracts. Importantly, we generated an MAb that is capable of neutralizing endogenous Gwl. The addition of this antibody into M-phase extracts results in loss of mitotic phosphorylation of Gwl, Plk1, and Cdk1 substrates. These results illustrate a new tool to study loss-of-function of Gwl, and support its essential role in mitosis. Finally, we demonstrated the usefulness of the MAb against human Gwl/MASTL.

Original languageEnglish (US)
Pages (from-to)469-474
Number of pages6
JournalHybridoma
Volume30
Issue number5
DOIs
StatePublished - Oct 1 2011

Fingerprint

Xenopus
Phosphotransferases
Monoclonal Antibodies
Mitosis
Cell Division
Aurora Kinases
Antibodies
Immunoprecipitation
Immunoblotting
Protein Kinases
Ovum
Cell Cycle
Maintenance
Phosphorylation
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Monoclonal antibodies against xenopus greatwall kinase. / Wang, Ling; Fisher, Laura A.; Wahl, James K; Peng, Aimin.

In: Hybridoma, Vol. 30, No. 5, 01.10.2011, p. 469-474.

Research output: Contribution to journalArticle

Wang, Ling ; Fisher, Laura A. ; Wahl, James K ; Peng, Aimin. / Monoclonal antibodies against xenopus greatwall kinase. In: Hybridoma. 2011 ; Vol. 30, No. 5. pp. 469-474.
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