Monocarboxylate transporter 1 mediates biotin uptake in human peripheral blood mononuclear cells

Rachel L. Daberkow, Brett R White, Rebecca A. Cederberg, Jacob B. Griffin, Janos Zempleni

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Here the hypothesis was tested that monocarboxylate transporters (MCT) mediate biotin transport in human lymphoid cells. Uptake of [ 3H]biotin was measured in human lymphoid cells [peripheral blood mononuclear cells (PBMC) and Jurkat cells] under conditions known to affect MCT-mediated transport. When biotin uptake into PBMC was measured in the presence of excess concentrations of competitors (MCT substrates) and MCT inhibitors, transport rates decreased significantly to <75 and <67%, respectively, of controls. Biotin uptake correlated with the concentration of protons in culture media, consistent with cotransport of protons and the carboxylate biotin by MCT. Efflux of biotin from PBMC was stimulated by extracellular lactate (a known substrate for MCT), consistent with countertransport of the two substrates by the same transporter. PBMC responded to proliferation with parallel increases of transport rates for both biotin and lactate, providing circumstantial evidence that the same transporter mediates uptake of the two substrates in PBMC. Transfection of Jurkat cells with an expression vector encoding MCT1 caused a 50% increase in biotin uptake; in contrast, overexpression of MCT1 did not affect biotin uptake in various nonlymphoid cell lines. These findings are consistent with the hypothesis that MCT mediate biotin uptake in human lymphoid cells.

Original languageEnglish (US)
Pages (from-to)2703-2706
Number of pages4
JournalJournal of Nutrition
Volume133
Issue number9
StatePublished - Sep 1 2003

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biotin
Biotin
mononuclear leukocytes
transporters
Blood Cells
Jurkat Cells
Lymphocytes
Protons
Lactic Acid
protons
lactates
cells
biotin 1
Transfection
Culture Media
transfection
Cell Line
culture media
cell lines
biotin transporter

Keywords

  • Biotin
  • Human
  • Monocarboxylate transporter
  • Peripheral blood mononuclear cells
  • Transport

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Monocarboxylate transporter 1 mediates biotin uptake in human peripheral blood mononuclear cells. / Daberkow, Rachel L.; White, Brett R; Cederberg, Rebecca A.; Griffin, Jacob B.; Zempleni, Janos.

In: Journal of Nutrition, Vol. 133, No. 9, 01.09.2003, p. 2703-2706.

Research output: Contribution to journalArticle

Daberkow, Rachel L. ; White, Brett R ; Cederberg, Rebecca A. ; Griffin, Jacob B. ; Zempleni, Janos. / Monocarboxylate transporter 1 mediates biotin uptake in human peripheral blood mononuclear cells. In: Journal of Nutrition. 2003 ; Vol. 133, No. 9. pp. 2703-2706.
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