Monoamine Oxidase b prompts mitochondrial and cardiac dysfunction in pressure overloaded hearts

Nina Kaludercic, Andrea Carpi, Takahiro Nagayama, Vidhya Sivakumaran, Guangshuo Zhu, Edwin W. Lai, Djahida Bedja, Agnese De Mario, Kevin Chen, Kathleen L. Gabrielson, Merry L. Lindsey, Karel Pacak, Eiki Takimoto, Jean C. Shih, David A. Kass, Fabio Di Lisa, Nazareno Paolocci

Research output: Contribution to journalArticle

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Abstract

Aims: Monoamine oxidases (MAOs) are mitochondrial flavoenzymes responsible for neurotransmitter and biogenic amines catabolism. MAO-A contributes to heart failure progression via enhanced norepinephrine catabolism and oxidative stress. The potential pathogenetic role of the isoenzyme MAO-B in cardiac diseases is currently unknown. Moreover, it is has not been determined yet whether MAO activation can directly affect mitochondrial function. Results: In wild type mice, pressure overload induced by transverse aortic constriction (TAC) resulted in enhanced dopamine catabolism, left ventricular (LV) remodeling, and dysfunction. Conversely, mice lacking MAO-B (MAO-B-/-) subjected to TAC maintained concentric hypertrophy accompanied by extracellular signal regulated kinase (ERK)1/2 activation, and preserved LV function, both at early (3 weeks) and late stages (9 weeks). Enhanced MAO activation triggered oxidative stress, and dropped mitochondrial membrane potential in the presence of ATP synthase inhibitor oligomycin both in neonatal and adult cardiomyocytes. The MAO-B inhibitor pargyline completely offset this change, suggesting that MAO activation induces a latent mitochondrial dysfunction, causing these organelles to hydrolyze ATP. Moreover, MAO-dependent aldehyde formation due to inhibition of aldehyde dehydrogenase 2 activity also contributed to alter mitochondrial bioenergetics. Innovation: Our study unravels a novel role for MAO-B in the pathogenesis of heart failure, showing that both MAO-driven reactive oxygen species production and impaired aldehyde metabolism affect mitochondrial function. Conclusion: Under conditions of chronic hemodynamic stress, enhanced MAO-B activity is a major determinant of cardiac structural and functional disarrangement. Both increased oxidative stress and the accumulation of aldehyde intermediates are likely liable for these adverse morphological and mechanical changes by directly targeting mitochondria. Antioxid. Redox Signal. 20, 267-280.

Original languageEnglish (US)
Pages (from-to)267-280
Number of pages14
JournalAntioxidants and Redox Signaling
Volume20
Issue number2
DOIs
StatePublished - Jan 13 2014

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Monoamine Oxidase
Pressure
Oxidative stress
Chemical activation
Aldehydes
Oxidative Stress
Constriction
Heart Failure
Adenosine Triphosphate
Pargyline
Oligomycins
Aldehyde Dehydrogenase
Ventricular Remodeling
Mitochondria
Biogenic Amines
Monoamine Oxidase Inhibitors
Mitogen-Activated Protein Kinase 3
Mitochondrial Membrane Potential
Mitogen-Activated Protein Kinase 1
Hemodynamics

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Kaludercic, N., Carpi, A., Nagayama, T., Sivakumaran, V., Zhu, G., Lai, E. W., ... Paolocci, N. (2014). Monoamine Oxidase b prompts mitochondrial and cardiac dysfunction in pressure overloaded hearts. Antioxidants and Redox Signaling, 20(2), 267-280. https://doi.org/10.1089/ars.2012.4616

Monoamine Oxidase b prompts mitochondrial and cardiac dysfunction in pressure overloaded hearts. / Kaludercic, Nina; Carpi, Andrea; Nagayama, Takahiro; Sivakumaran, Vidhya; Zhu, Guangshuo; Lai, Edwin W.; Bedja, Djahida; De Mario, Agnese; Chen, Kevin; Gabrielson, Kathleen L.; Lindsey, Merry L.; Pacak, Karel; Takimoto, Eiki; Shih, Jean C.; Kass, David A.; Di Lisa, Fabio; Paolocci, Nazareno.

In: Antioxidants and Redox Signaling, Vol. 20, No. 2, 13.01.2014, p. 267-280.

Research output: Contribution to journalArticle

Kaludercic, N, Carpi, A, Nagayama, T, Sivakumaran, V, Zhu, G, Lai, EW, Bedja, D, De Mario, A, Chen, K, Gabrielson, KL, Lindsey, ML, Pacak, K, Takimoto, E, Shih, JC, Kass, DA, Di Lisa, F & Paolocci, N 2014, 'Monoamine Oxidase b prompts mitochondrial and cardiac dysfunction in pressure overloaded hearts', Antioxidants and Redox Signaling, vol. 20, no. 2, pp. 267-280. https://doi.org/10.1089/ars.2012.4616
Kaludercic, Nina ; Carpi, Andrea ; Nagayama, Takahiro ; Sivakumaran, Vidhya ; Zhu, Guangshuo ; Lai, Edwin W. ; Bedja, Djahida ; De Mario, Agnese ; Chen, Kevin ; Gabrielson, Kathleen L. ; Lindsey, Merry L. ; Pacak, Karel ; Takimoto, Eiki ; Shih, Jean C. ; Kass, David A. ; Di Lisa, Fabio ; Paolocci, Nazareno. / Monoamine Oxidase b prompts mitochondrial and cardiac dysfunction in pressure overloaded hearts. In: Antioxidants and Redox Signaling. 2014 ; Vol. 20, No. 2. pp. 267-280.
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AU - Carpi, Andrea

AU - Nagayama, Takahiro

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AU - Zhu, Guangshuo

AU - Lai, Edwin W.

AU - Bedja, Djahida

AU - De Mario, Agnese

AU - Chen, Kevin

AU - Gabrielson, Kathleen L.

AU - Lindsey, Merry L.

AU - Pacak, Karel

AU - Takimoto, Eiki

AU - Shih, Jean C.

AU - Kass, David A.

AU - Di Lisa, Fabio

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