Monitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system

Nayeon Lee, Jae Woo Park, Hyung Joon Kim, Ju Hun Yeon, Jihye Kwon, Jung Jae Ko, Seung Hun Oh, Hyun Sook Kim, Aeri Kim, Baek Soo Han, Sang Chul Lee, Noo Li Jeon, Jihwan Song

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Microfluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin-positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.

Original languageEnglish (US)
Pages (from-to)497-502
Number of pages6
JournalMolecules and Cells
Volume37
Issue number6
DOIs
StatePublished - Jun 2014

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Microfluidics
Technology
Nestin
Stromal Cells
Cell Movement
Axons
Suspensions
Equipment and Supplies
Human Embryonic Stem Cells
Growth
Axon Guidance

Keywords

  • Axons
  • Human embryonic stem cells
  • Microfluidics
  • Migration
  • Neural differentiation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Monitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system. / Lee, Nayeon; Park, Jae Woo; Kim, Hyung Joon; Yeon, Ju Hun; Kwon, Jihye; Ko, Jung Jae; Oh, Seung Hun; Kim, Hyun Sook; Kim, Aeri; Han, Baek Soo; Lee, Sang Chul; Jeon, Noo Li; Song, Jihwan.

In: Molecules and Cells, Vol. 37, No. 6, 06.2014, p. 497-502.

Research output: Contribution to journalArticle

Lee, N, Park, JW, Kim, HJ, Yeon, JH, Kwon, J, Ko, JJ, Oh, SH, Kim, HS, Kim, A, Han, BS, Lee, SC, Jeon, NL & Song, J 2014, 'Monitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system', Molecules and Cells, vol. 37, no. 6, pp. 497-502. https://doi.org/10.14348/molcells.2014.0137
Lee, Nayeon ; Park, Jae Woo ; Kim, Hyung Joon ; Yeon, Ju Hun ; Kwon, Jihye ; Ko, Jung Jae ; Oh, Seung Hun ; Kim, Hyun Sook ; Kim, Aeri ; Han, Baek Soo ; Lee, Sang Chul ; Jeon, Noo Li ; Song, Jihwan. / Monitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system. In: Molecules and Cells. 2014 ; Vol. 37, No. 6. pp. 497-502.
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