Monitoring demineralization and remineralization of human dentin by characterization of its structure with resonance-enhanced AFM-IR chemical mapping, nanoindentation, and SEM

Grigoriy Sereda, Allison VanLaecken, Joseph Alan Turner

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: This research aimed at monitoring demineralization and remineralization of dentin and its collagen matrix at the nanoscale by amorphous, microcrystalline, and in situ formed hydroxyapatite. Methods: The concurrent use of the resonance-enhanced atomic force microscopy coupled with infrared probe (AFM-IR) chemical mapping, nano-indentation, and scanning electron microscopy (SEM) provides a detailed insight into the structure of human dentin, as well as to the processes of its partial demineralization and remineralization. Results: The resonance-enhanced AFM-IR chemical mapping of dentin has shown to be a useful method to follow distribution of its collagen and hydroxyapatite components at the micro- and nanoscale levels, especially in conjunction with SEM imaging and nanoindentation. Dentin with a higher extent of natural dentin tubule occlusion tends to be harder and less elastic. The relative affinity of the collagen and hydroxyapatite components of dentin toward hydroxyapatite depends on its type (amorphous, microcrystalline, or formed in-situ). The gel mineralization technique allows for an even and controlled growth of hydroxyapatite guided by the completely demineralized collagen matrix of dentin. Significance: The observed trends of the affinity of collagen toward different forms of hydroxyapatite helps develop new remineralizing formulations. The employed methods of characterization may provide an insight to the natural processes of bone mineralization guided by its both hydroxyapatite and protein constituents.

Original languageEnglish (US)
Pages (from-to)617-626
Number of pages10
JournalDental Materials
Volume35
Issue number4
DOIs
StatePublished - Apr 2019

Fingerprint

Dentin
Nanoindentation
Durapatite
Hydroxyapatite
Electron Scanning Microscopy
Collagen
Scanning electron microscopy
Monitoring
Physiologic Calcification
Atomic Force Microscopy
Atomic force microscopy
Bone
Gels
Infrared radiation
Proteins
Imaging techniques
Growth
Research

Keywords

  • Atomic force microscopy
  • Collagen matrix
  • Dentin
  • Nanoindentation
  • Scanning electron microscopy
  • Tooth remineralization

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

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title = "Monitoring demineralization and remineralization of human dentin by characterization of its structure with resonance-enhanced AFM-IR chemical mapping, nanoindentation, and SEM",
abstract = "Objective: This research aimed at monitoring demineralization and remineralization of dentin and its collagen matrix at the nanoscale by amorphous, microcrystalline, and in situ formed hydroxyapatite. Methods: The concurrent use of the resonance-enhanced atomic force microscopy coupled with infrared probe (AFM-IR) chemical mapping, nano-indentation, and scanning electron microscopy (SEM) provides a detailed insight into the structure of human dentin, as well as to the processes of its partial demineralization and remineralization. Results: The resonance-enhanced AFM-IR chemical mapping of dentin has shown to be a useful method to follow distribution of its collagen and hydroxyapatite components at the micro- and nanoscale levels, especially in conjunction with SEM imaging and nanoindentation. Dentin with a higher extent of natural dentin tubule occlusion tends to be harder and less elastic. The relative affinity of the collagen and hydroxyapatite components of dentin toward hydroxyapatite depends on its type (amorphous, microcrystalline, or formed in-situ). The gel mineralization technique allows for an even and controlled growth of hydroxyapatite guided by the completely demineralized collagen matrix of dentin. Significance: The observed trends of the affinity of collagen toward different forms of hydroxyapatite helps develop new remineralizing formulations. The employed methods of characterization may provide an insight to the natural processes of bone mineralization guided by its both hydroxyapatite and protein constituents.",
keywords = "Atomic force microscopy, Collagen matrix, Dentin, Nanoindentation, Scanning electron microscopy, Tooth remineralization",
author = "Grigoriy Sereda and Allison VanLaecken and Turner, {Joseph Alan}",
year = "2019",
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T1 - Monitoring demineralization and remineralization of human dentin by characterization of its structure with resonance-enhanced AFM-IR chemical mapping, nanoindentation, and SEM

AU - Sereda, Grigoriy

AU - VanLaecken, Allison

AU - Turner, Joseph Alan

PY - 2019/4

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N2 - Objective: This research aimed at monitoring demineralization and remineralization of dentin and its collagen matrix at the nanoscale by amorphous, microcrystalline, and in situ formed hydroxyapatite. Methods: The concurrent use of the resonance-enhanced atomic force microscopy coupled with infrared probe (AFM-IR) chemical mapping, nano-indentation, and scanning electron microscopy (SEM) provides a detailed insight into the structure of human dentin, as well as to the processes of its partial demineralization and remineralization. Results: The resonance-enhanced AFM-IR chemical mapping of dentin has shown to be a useful method to follow distribution of its collagen and hydroxyapatite components at the micro- and nanoscale levels, especially in conjunction with SEM imaging and nanoindentation. Dentin with a higher extent of natural dentin tubule occlusion tends to be harder and less elastic. The relative affinity of the collagen and hydroxyapatite components of dentin toward hydroxyapatite depends on its type (amorphous, microcrystalline, or formed in-situ). The gel mineralization technique allows for an even and controlled growth of hydroxyapatite guided by the completely demineralized collagen matrix of dentin. Significance: The observed trends of the affinity of collagen toward different forms of hydroxyapatite helps develop new remineralizing formulations. The employed methods of characterization may provide an insight to the natural processes of bone mineralization guided by its both hydroxyapatite and protein constituents.

AB - Objective: This research aimed at monitoring demineralization and remineralization of dentin and its collagen matrix at the nanoscale by amorphous, microcrystalline, and in situ formed hydroxyapatite. Methods: The concurrent use of the resonance-enhanced atomic force microscopy coupled with infrared probe (AFM-IR) chemical mapping, nano-indentation, and scanning electron microscopy (SEM) provides a detailed insight into the structure of human dentin, as well as to the processes of its partial demineralization and remineralization. Results: The resonance-enhanced AFM-IR chemical mapping of dentin has shown to be a useful method to follow distribution of its collagen and hydroxyapatite components at the micro- and nanoscale levels, especially in conjunction with SEM imaging and nanoindentation. Dentin with a higher extent of natural dentin tubule occlusion tends to be harder and less elastic. The relative affinity of the collagen and hydroxyapatite components of dentin toward hydroxyapatite depends on its type (amorphous, microcrystalline, or formed in-situ). The gel mineralization technique allows for an even and controlled growth of hydroxyapatite guided by the completely demineralized collagen matrix of dentin. Significance: The observed trends of the affinity of collagen toward different forms of hydroxyapatite helps develop new remineralizing formulations. The employed methods of characterization may provide an insight to the natural processes of bone mineralization guided by its both hydroxyapatite and protein constituents.

KW - Atomic force microscopy

KW - Collagen matrix

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KW - Scanning electron microscopy

KW - Tooth remineralization

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