Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting.

Clayton L Kelling, J. E. Kennedy, K. K. Rump, L. C. Stine, P. S. Paul, J. E. Partridge

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.

Original languageEnglish (US)
Pages (from-to)1237-1244
Number of pages8
JournalAmerican journal of veterinary research
Volume52
Issue number8
StatePublished - Aug 1991

Fingerprint

Ribonuclease T1
Bovine Viral Diarrhea Viruses
ribonucleases
Bovine viral diarrhea virus
Viral RNA
oligonucleotides
Oligonucleotides
Vaccines
RNA
vaccines
Viruses
viruses
monitoring
Bovine viral diarrhea virus 1
cell culture
Cell Culture Techniques
Dermatoglyphics
purity

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting. / Kelling, Clayton L; Kennedy, J. E.; Rump, K. K.; Stine, L. C.; Paul, P. S.; Partridge, J. E.

In: American journal of veterinary research, Vol. 52, No. 8, 08.1991, p. 1237-1244.

Research output: Contribution to journalArticle

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AU - Paul, P. S.

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