Molecular systematics of the neotropical genus psiguria (cucurbitaceae)

Implications for phylogeny and species identification

Pamela R Kellar, Lauren M. Friar, Lawrence E. Gilbert, Robert K. Jansen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Varying morphological features in many groups of tropical vines confound identification, requiring molecular tools for distinguishing species. Confusion is amplified in Psiguria, a small genus found in Central and South America and the Caribbean, because male and female flowers of these monoecious plants are widely separated by time and position on a branch. We present the first phylogeny of Psiguria utilizing a combination of eight chloroplast intergenic spacers, the internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA repeat, and the intron of the low-copy nuclear gene serine/threonine phosphatase, for a total aligned length of 9456 base pairs. Analyses include multiple accessions of all species in the genus. The data support the monophyly of Psiguria and elucidate several species boundaries. Also presented are Psiguria-specific DNA barcodes, which include the chloroplast regions: ndhC-trnV, rps16-trnQ, rpoB-trnC, ndhF-rpl32, and psbZ-trnM. For the first time, systematists, ecologists, and evolutionary biologists will have the tools to confidently identify species of Psiguria with DNA barcodes that may be useful in other genera of Cucurbitaceae.

Original languageEnglish (US)
Pages (from-to)156-173
Number of pages18
JournalAmerican Journal of Botany
Volume97
Issue number1
DOIs
StatePublished - Jan 1 2010

Fingerprint

Cucurbitaceae
molecular systematics
DNA barcoding
Chloroplasts
Phylogeny
phylogeny
chloroplasts
Genomic Segmental Duplications
Central America
male flowers
female flowers
South America
Phosphoprotein Phosphatases
DNA
Ribosomal DNA
ecologists
intergenic DNA
threonine
chloroplast
Base Pairing

Keywords

  • Angiosperms
  • Caribbean
  • Chloroplast
  • Dna barcoding
  • Gurania
  • Guraniinae
  • Helmontia
  • Its
  • Low-copy nuclear
  • Phylogenetics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

Cite this

Molecular systematics of the neotropical genus psiguria (cucurbitaceae) : Implications for phylogeny and species identification. / Kellar, Pamela R; Friar, Lauren M.; Gilbert, Lawrence E.; Jansen, Robert K.

In: American Journal of Botany, Vol. 97, No. 1, 01.01.2010, p. 156-173.

Research output: Contribution to journalArticle

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