Molecular recognition via triplex formation of mixed purine/pyrimidine DNA sequences using oligoTRIPs

Jian Sen Li, Fa Xian Chen, Ronald Shikiya, Luis A Marky, Barry Gold

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28 Scopus citations


Stable DNA triple-helical structures are normally restricted to homopurine sequences. We have described a system of four heterocyclic bases (TRIPsides) that, when incorporated into oligomers (oligoTRIPs), can recognize and bind in the major groove to any native sequence of DNA [Li et al., J. Am. Chem. Soc. 2003, 125, 2084]. To date, we have reported on triplex-forming oligomers composed of two of these TRIPsides, i.e., antiTA and antiGC, and their ability to form intramolecular triplexes at mixed purine/ pyrimidine sequences. In the present study, we describe the synthesis and characterization of the antiCG TRIPside and its use in conjunction with antiTA and antiGC to form sequence-specific intra- and/or intermolecular triplex structures at mixed purine/pyrimidine sequences that require as many as four major groove crossovers.

Original languageEnglish (US)
Pages (from-to)12657-12665
Number of pages9
JournalJournal of the American Chemical Society
Issue number36
Publication statusPublished - Sep 14 2005


ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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