Molecular probes define different regions of the mouse t complex

Howard S. Fox, Gail R. Martin, Mary F. Lyon, Bernhard Herrmann, Anna Maria Frischauf, Hans Lehrach, Lee M. Silver

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Abstract

Four genomic clones obtained from microdissected fragments of the proximal portion of mouse chromosome 17 have been used to identify a series of t-haplotype-specific restriction fragments. Their specificity is defined by presence in eight complete t haplotypes and absence from 18 inbred strains of wild-type mice. Partial t haplotypes contain subsets of the t-specific fragments, and each can be classified according to the t-specific fragments it contains. This is the first molecular evidence that independent partial t haplotypes contain different lengths of t haplotype DNA. Recombination studies indicate that partial t haplotypes suppress recombination in proportion to the extent of t haplotype DNA they contain. Molecular analysis of partial t haplotyes shows that the t-specific fragments map to and thus define different regions of the t complex. Certain regions of t haplotype DNA defined by t-specific restriction fragments can be correlated with loci involved in the control of transmission ratio distortion.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalCell
Volume40
Issue number1
DOIs
StatePublished - Jan 1985

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fox, H. S., Martin, G. R., Lyon, M. F., Herrmann, B., Frischauf, A. M., Lehrach, H., & Silver, L. M. (1985). Molecular probes define different regions of the mouse t complex. Cell, 40(1), 63-69. https://doi.org/10.1016/0092-8674(85)90309-5