Molecular phylogeny and genome evolution in the Drosophila virilis species group

Duplications of the alcohol Dehydrogenase gene

D. I. Nurminsky, Etsuko Moriyama, E. R. Lozovskaya, D. L. Hartl

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Drosophila virilis is a prominent reference species for comparison with Drosophila melanogaster in regard to patterns and mechanisms of molecular and genomic evolution. Sequences were determined for 11 Adh genes from 8 species of the D. virilis species group, including species from both the virilis phylad and the montana subphylad. The genome of D. virilis contains a 6-kb duplication that includes the entire Adh coding region. The pattern of sequence identity within the duplication strongly suggests a recent gene- conversion event bordered by 36-bp indels. As in other Drosophila, the amino- acid coding region of Adh is encoded by three exons interrupted by two short introns. The promoter region includes 16 blocks of sequence that are well conserved in D. virilis, Drosophila hydei, and D. melanogaster. The developmental profile of Adh transcription suggests a distal/proximal promoter switch analogous to that in D. melanogaster. Duplicate Adh genes were also found in Drosophila montana and Drosophila lacicola, which apparently originated independently of that in D. virilis. The Adh genes in all species of the D. virilis group have among the lowest codon bias of any Adh genes so far reported in the genus Drosophila. Taking the low codon bias into account, we estimate the time of divergence between the virilis and montana clades as 9.0 ± 0.7 Mya and the approximate time of divergence of D. virilis from other members of the virilis phylad as 2.6 ± 0.4 Mya. The region of the D. virilis genome containing Adh, as well as the chromosome as a whole, gives evidence of extensive rearrangements relative to the genome of D. melanogaster.

Original languageEnglish (US)
Pages (from-to)132-149
Number of pages18
JournalMolecular biology and evolution
Volume13
Issue number1
DOIs
StatePublished - Jan 1 1996

Fingerprint

Drosophila virilis
Alcohol Dehydrogenase
alcohol dehydrogenase
Phylogeny
Drosophila
alcohol
phylogeny
genome
Genes
Genome
Drosophila melanogaster
gene
Mya
genes
Codon
divergence
Duplicate Genes
codons
Gene Conversion
Molecular Evolution

Keywords

  • Adh gene
  • Alcohol dehydrogenase
  • D. virilis species group
  • Drosophila virilis
  • chromosomal inversion
  • genome evolution

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Molecular phylogeny and genome evolution in the Drosophila virilis species group : Duplications of the alcohol Dehydrogenase gene. / Nurminsky, D. I.; Moriyama, Etsuko; Lozovskaya, E. R.; Hartl, D. L.

In: Molecular biology and evolution, Vol. 13, No. 1, 01.01.1996, p. 132-149.

Research output: Contribution to journalArticle

@article{113b691dee2e49f4bb3e6fb1a41fea7a,
title = "Molecular phylogeny and genome evolution in the Drosophila virilis species group: Duplications of the alcohol Dehydrogenase gene",
abstract = "Drosophila virilis is a prominent reference species for comparison with Drosophila melanogaster in regard to patterns and mechanisms of molecular and genomic evolution. Sequences were determined for 11 Adh genes from 8 species of the D. virilis species group, including species from both the virilis phylad and the montana subphylad. The genome of D. virilis contains a 6-kb duplication that includes the entire Adh coding region. The pattern of sequence identity within the duplication strongly suggests a recent gene- conversion event bordered by 36-bp indels. As in other Drosophila, the amino- acid coding region of Adh is encoded by three exons interrupted by two short introns. The promoter region includes 16 blocks of sequence that are well conserved in D. virilis, Drosophila hydei, and D. melanogaster. The developmental profile of Adh transcription suggests a distal/proximal promoter switch analogous to that in D. melanogaster. Duplicate Adh genes were also found in Drosophila montana and Drosophila lacicola, which apparently originated independently of that in D. virilis. The Adh genes in all species of the D. virilis group have among the lowest codon bias of any Adh genes so far reported in the genus Drosophila. Taking the low codon bias into account, we estimate the time of divergence between the virilis and montana clades as 9.0 ± 0.7 Mya and the approximate time of divergence of D. virilis from other members of the virilis phylad as 2.6 ± 0.4 Mya. The region of the D. virilis genome containing Adh, as well as the chromosome as a whole, gives evidence of extensive rearrangements relative to the genome of D. melanogaster.",
keywords = "Adh gene, Alcohol dehydrogenase, D. virilis species group, Drosophila virilis, chromosomal inversion, genome evolution",
author = "Nurminsky, {D. I.} and Etsuko Moriyama and Lozovskaya, {E. R.} and Hartl, {D. L.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1093/oxfordjournals.molbev.a025551",
language = "English (US)",
volume = "13",
pages = "132--149",
journal = "Molecular Biology and Evolution",
issn = "0737-4038",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Molecular phylogeny and genome evolution in the Drosophila virilis species group

T2 - Duplications of the alcohol Dehydrogenase gene

AU - Nurminsky, D. I.

AU - Moriyama, Etsuko

AU - Lozovskaya, E. R.

AU - Hartl, D. L.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Drosophila virilis is a prominent reference species for comparison with Drosophila melanogaster in regard to patterns and mechanisms of molecular and genomic evolution. Sequences were determined for 11 Adh genes from 8 species of the D. virilis species group, including species from both the virilis phylad and the montana subphylad. The genome of D. virilis contains a 6-kb duplication that includes the entire Adh coding region. The pattern of sequence identity within the duplication strongly suggests a recent gene- conversion event bordered by 36-bp indels. As in other Drosophila, the amino- acid coding region of Adh is encoded by three exons interrupted by two short introns. The promoter region includes 16 blocks of sequence that are well conserved in D. virilis, Drosophila hydei, and D. melanogaster. The developmental profile of Adh transcription suggests a distal/proximal promoter switch analogous to that in D. melanogaster. Duplicate Adh genes were also found in Drosophila montana and Drosophila lacicola, which apparently originated independently of that in D. virilis. The Adh genes in all species of the D. virilis group have among the lowest codon bias of any Adh genes so far reported in the genus Drosophila. Taking the low codon bias into account, we estimate the time of divergence between the virilis and montana clades as 9.0 ± 0.7 Mya and the approximate time of divergence of D. virilis from other members of the virilis phylad as 2.6 ± 0.4 Mya. The region of the D. virilis genome containing Adh, as well as the chromosome as a whole, gives evidence of extensive rearrangements relative to the genome of D. melanogaster.

AB - Drosophila virilis is a prominent reference species for comparison with Drosophila melanogaster in regard to patterns and mechanisms of molecular and genomic evolution. Sequences were determined for 11 Adh genes from 8 species of the D. virilis species group, including species from both the virilis phylad and the montana subphylad. The genome of D. virilis contains a 6-kb duplication that includes the entire Adh coding region. The pattern of sequence identity within the duplication strongly suggests a recent gene- conversion event bordered by 36-bp indels. As in other Drosophila, the amino- acid coding region of Adh is encoded by three exons interrupted by two short introns. The promoter region includes 16 blocks of sequence that are well conserved in D. virilis, Drosophila hydei, and D. melanogaster. The developmental profile of Adh transcription suggests a distal/proximal promoter switch analogous to that in D. melanogaster. Duplicate Adh genes were also found in Drosophila montana and Drosophila lacicola, which apparently originated independently of that in D. virilis. The Adh genes in all species of the D. virilis group have among the lowest codon bias of any Adh genes so far reported in the genus Drosophila. Taking the low codon bias into account, we estimate the time of divergence between the virilis and montana clades as 9.0 ± 0.7 Mya and the approximate time of divergence of D. virilis from other members of the virilis phylad as 2.6 ± 0.4 Mya. The region of the D. virilis genome containing Adh, as well as the chromosome as a whole, gives evidence of extensive rearrangements relative to the genome of D. melanogaster.

KW - Adh gene

KW - Alcohol dehydrogenase

KW - D. virilis species group

KW - Drosophila virilis

KW - chromosomal inversion

KW - genome evolution

UR - http://www.scopus.com/inward/record.url?scp=0030032993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030032993&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.molbev.a025551

DO - 10.1093/oxfordjournals.molbev.a025551

M3 - Article

VL - 13

SP - 132

EP - 149

JO - Molecular Biology and Evolution

JF - Molecular Biology and Evolution

SN - 0737-4038

IS - 1

ER -