Molecular genetic and biochemical characterization of the vaccinia virus I3 protein, the replicative single-stranded DNA binding protein

Matthew D. Greseth, Kathleen A. Boyle, Matthew S. Bluma, Bethany Unger, Matthew S Wiebe, Jamaria A. Soares-Martins, Nadi T. Wickramasekera, James Wahlberg, Paula Traktman

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Vaccinia virus, the prototypic poxvirus, efficiently and faithfully replicates its~200-kb DNA genome within the cytoplasm of infected cells. This intracellular localization dictates that vaccinia virus encodes most, if not all, of its own DNA replication machinery. Included in the repertoire of viral replication proteins is the I3 protein, which binds to single-stranded DNA (ssDNA) with great specificity and stability and has been presumed to be the replicative ssDNA binding protein (SSB). We substantiate here that I3 colocalizes with bromodeoxyuridine (BrdU)-labeled nascent viral genomes and that these genomes accumulate in cytoplasmic factories that are delimited by membranes derived from the endoplasmic reticulum. Moreover, we report on a structure/function analysis of I3 involving the isolation and characterization of 10 clustered charge-to-alanine mutants. These mutants were analyzed for their biochemical properties (self-interaction and DNA binding) and biological competence. Three of the mutant proteins, encoded by the I3 alleles I3-4, -5, and -7, were deficient in self-interaction and unable to support virus viability, strongly suggesting that the multimerization of I3 is biologically significant. Mutant I3-5 was also deficient in DNA binding. Additionally, we demonstrate that small interfering RNA (siRNA)-mediated depletion of I3 causes a significant decrease in the accumulation of progeny genomes and that this reduction diminishes the yield of infectious virus.

Original languageEnglish (US)
Pages (from-to)6197-6209
Number of pages13
JournalJournal of virology
Volume86
Issue number11
DOIs
StatePublished - Jun 1 2012

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Vaccinia virus
single-stranded DNA
DNA-binding proteins
DNA-Binding Proteins
molecular genetics
Molecular Biology
Genome
mutants
genome
DNA
Microbial Viability
Poxviridae
proteins
Viral Genome
Single-Stranded DNA
Viral Proteins
Mutant Proteins
Bromodeoxyuridine
DNA Replication
Endoplasmic Reticulum

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Molecular genetic and biochemical characterization of the vaccinia virus I3 protein, the replicative single-stranded DNA binding protein. / Greseth, Matthew D.; Boyle, Kathleen A.; Bluma, Matthew S.; Unger, Bethany; Wiebe, Matthew S; Soares-Martins, Jamaria A.; Wickramasekera, Nadi T.; Wahlberg, James; Traktman, Paula.

In: Journal of virology, Vol. 86, No. 11, 01.06.2012, p. 6197-6209.

Research output: Contribution to journalArticle

Greseth, MD, Boyle, KA, Bluma, MS, Unger, B, Wiebe, MS, Soares-Martins, JA, Wickramasekera, NT, Wahlberg, J & Traktman, P 2012, 'Molecular genetic and biochemical characterization of the vaccinia virus I3 protein, the replicative single-stranded DNA binding protein', Journal of virology, vol. 86, no. 11, pp. 6197-6209. https://doi.org/10.1128/JVI.00206-12
Greseth, Matthew D. ; Boyle, Kathleen A. ; Bluma, Matthew S. ; Unger, Bethany ; Wiebe, Matthew S ; Soares-Martins, Jamaria A. ; Wickramasekera, Nadi T. ; Wahlberg, James ; Traktman, Paula. / Molecular genetic and biochemical characterization of the vaccinia virus I3 protein, the replicative single-stranded DNA binding protein. In: Journal of virology. 2012 ; Vol. 86, No. 11. pp. 6197-6209.
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