Molecular cloning of cDNAs for 14-3-3 and its protein interactions in a white-rot fungus Phanerochaete chrysosporium

Guoku Hu, Hong Feng, Tao Zhang, Yonghong Yan, Bo Wu, Quan Jiang, Jinming Wu, Yizheng Zhang

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The 14-3-3 is a highly conserved, ubiquitous expressed protein in eukaryotes and very important in the regulation of such crucial cellular processes as metabolism, signal transduction, cell-cycle control, apoptosis, protein trafficking, transcription, stress responses, and malignant transformation. In this study, the full-length cDNA of 14-3-3 was cloned first time from a white-rot fungus Phanerochaete chrysosporium. Yeast two hybrid has been performed to fish out the proteins that can bind with 14-3-3 in P. chrysosporium. The results showed that 14-3-3 could form homodimers in P. chrysosporium. Two novel proteins containing predicted WD domain could interact with 14-3-3, too. In addition, the transcription of 14-3-3 gene under low-nitrogen medium was constitutional by RT-PCR analysis. These results indicate that 14-3-3 protein in P. chrysosporium may be involved in multiple cellular processes.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalAnnals of Microbiology
Volume56
Issue number3
DOIs
Publication statusPublished - Jan 1 2006

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Keywords

  • 14-3-3
  • Gene cloning
  • Phanerochaete chrysosporium
  • WD domain
  • Yeast two hybrid

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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