Molecular cloning and characterization of a peroxiredoxin from Phanerochaete chrysosporium

Quan Jiang, Yong Hong Yan, Guoku Hu, Yi Zheng Zhang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Peroxiredoxins (Prxs) are a ubiquitous family of peroxidases widely distributed among prokaryotes and eukaryotes. Here, we report on the cloning and functional characterization of a cDNA designated PcPrx-1, encoding peroxiredoxin from the white-rot fungus Phanerochaete chrysosporium. The full-length PcPrx-1 cDNA (932 bp) contains an open reading frame of 200 amino acid residues with a molecular mass of 22.1 kDa. The deduced primary structure of PcPrx-1 polypeptide shows a high level of sequence identity to other recently identified 2-cys peroxiredoxins. The recombinant PcPrx-1 protein was expressed as a histidine fusion protein in Escherichia coli and purified with a Ni 2+-column. The purified protein was shown to have a protective effect against plasmid DNA cleavage by reactive oxygen species. The PcPrx-1 protein displays the ability to remove H2O2 in a ferrithiocyanate system. The results of this study suggest that PcPrx-1 may play a protective role against oxidative stress in P. chrysosporium.

Original languageEnglish (US)
Pages (from-to)659-668
Number of pages10
JournalCellular and Molecular Biology Letters
Volume10
Issue number4
StatePublished - Jan 1 2005

Fingerprint

Phanerochaete
Peroxiredoxins
Cloning
Molecular Cloning
Complementary DNA
Peroxidases
DNA Cleavage
Proteins
Escherichia coli Proteins
Eukaryota
Histidine
Open Reading Frames
Oxidative stress
Organism Cloning
Reactive Oxygen Species
Molecular mass
Oxidative Stress
Plasmids
Fungi
Escherichia coli

Keywords

  • Cloning
  • Expression
  • Peroxiredoxin
  • Phanerochaete chrysosporium

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Molecular cloning and characterization of a peroxiredoxin from Phanerochaete chrysosporium. / Jiang, Quan; Yan, Yong Hong; Hu, Guoku; Zhang, Yi Zheng.

In: Cellular and Molecular Biology Letters, Vol. 10, No. 4, 01.01.2005, p. 659-668.

Research output: Contribution to journalArticle

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