Molecular characterization of two bacteriophages isolated from Desulfovibrio vulgaris NCIMB 8303 (Hildenborough)

S. Seyedirashti, Charles Wood, J. M. Akagi

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Abstract

A preliminary endonuclease restriction map of a bacteriophage isolated from Desulfovibrio vulgaris has been established. BamHI cleaved whole phage DNA into four fragments while HindIII cut the same DNA into seven fragments. Mapping studies succeeded in linking the four BamHI fragments into two DNA segments; however, no linkage between the two segments was detected. These data imply that two phages were induced from cultures of D. vulgaris and that the two segments represented the DNA from these phages. Support for this hypothesis came from size approximation of restriction enzyme fragments, electron micrographs, and density gradients.

Original languageEnglish (US)
Pages (from-to)1393-1397
Number of pages5
JournalJournal of General Microbiology
Volume138
Issue number7
DOIs
StatePublished - Jan 1 1992

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Desulfovibrio vulgaris
Bacteriophages
DNA
DNA Restriction Enzymes
Electrons
Enzymes

ASJC Scopus subject areas

  • Microbiology

Cite this

Molecular characterization of two bacteriophages isolated from Desulfovibrio vulgaris NCIMB 8303 (Hildenborough). / Seyedirashti, S.; Wood, Charles; Akagi, J. M.

In: Journal of General Microbiology, Vol. 138, No. 7, 01.01.1992, p. 1393-1397.

Research output: Contribution to journalArticle

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