Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and veterinary staff in China

Wanjiang Zhang, Zhihui Hao, Yang Wang, Xingyuan Cao, Catherine M. Logue, Bing Wang, Jing Yang, Jianzhong Shen, Congming Wu

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The aim of this study was to determine the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) isolates from pet animals and veterinary staff and the characteristics of these isolates. A total of 22 MRSA isolates were isolated from nasal swabs from dogs, cats and veterinary staff in six pet hospitals in six cities, and examined for antimicrobial susceptibility, the presence of resistance genes, Panton-Valentine leukocidin gene lukF-lukS, staphylococcal chromosomal cassette (SCC) mec typing, spa tying, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing. Of 22 MRSA isolates, 21 were recovered from pet animals, and one was isolated from a member of sstaff. All 22 MRSA strains were resistant to penicillin, oxacillin, azithromycin, clindamycin and ceftriaxone, and harboured mecA, ermB and linA genes. The lukF-lukS gene was not detected in any of the MRSA isolates. Eighteen MRSA strains from Qingdao belonged to ST59-MRSA-IV-spa t437. Of four MRSA isolates from Beijing, one belonged to ST398-MRSA-V-spa t034, and three belonged to ST239-MRSA-III-spa t030 profiles. Two PFGE types (A and B) were identified. Two isolates originating from dogs and one isolate originating from a staff member in Beijing shared similar PFGE patterns. Our cumulative data suggested that cross-transmission of MRSA may have occurred between pet animals and veterinary staff.

Original languageEnglish (US)
Pages (from-to)e125-e129
JournalVeterinary Journal
Volume190
Issue number2
DOIs
StatePublished - Nov 1 2011

Fingerprint

Pets
Methicillin-Resistant Staphylococcus aureus
pets
China
Pulsed Field Gel Electrophoresis
pulsed-field gel electrophoresis
Genes
methicillin-resistant Staphylococcus aureus
genes
Dogs
azithromycin
ceftriaxone
Linum
oxacillin
Multilocus Sequence Typing
Flax
Oxacillin
clindamycin
Azithromycin
Clindamycin

Keywords

  • Antimicrobial resistance
  • MLST
  • MRSA
  • Pet animals
  • SCCmec

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and veterinary staff in China. / Zhang, Wanjiang; Hao, Zhihui; Wang, Yang; Cao, Xingyuan; Logue, Catherine M.; Wang, Bing; Yang, Jing; Shen, Jianzhong; Wu, Congming.

In: Veterinary Journal, Vol. 190, No. 2, 01.11.2011, p. e125-e129.

Research output: Contribution to journalArticle

Zhang, Wanjiang ; Hao, Zhihui ; Wang, Yang ; Cao, Xingyuan ; Logue, Catherine M. ; Wang, Bing ; Yang, Jing ; Shen, Jianzhong ; Wu, Congming. / Molecular characterization of methicillin-resistant Staphylococcus aureus strains from pet animals and veterinary staff in China. In: Veterinary Journal. 2011 ; Vol. 190, No. 2. pp. e125-e129.
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