Molecular biology of human serum cholinesterase

B. N. La Du, O. Lockridge

Research output: Contribution to journalReview article

11 Citations (Scopus)

Abstract

More than 90% of the amino acid sequence of purified human serum cholinesterase has been determined in our laboratory. Purified enzyme was digested with several proteolytic enzymes; the resulting polypeptides were then separated, purified, and sequenced. Optimal sequence regions were identified and used as the basis for the synthesis of three 17-mer oligonucleotide probes. In addition, one long peptide of 58 amino acid residues was selected for construction of two unique sequence oligonucleotide probes of 39-mer and 53-mer; the peptide regions corresponding to the latter are six amino acids apart. The probes have been used to screen a human liver cDNA library and a human genomic library. Several positive clones to both types of probes have been identified. These are being characterized, and some of them have been or are now being sequenced. A high degree of homology in the amino acid sequence of the active center of human serum cholinesterase and that of acetylcholinesterse from the Torpedo fish has been noted. It appears that this region of cholinesterases has been conserved during evolution, and there may be an important, still unrecognized role for serum nonspecific cholinesterase in mammalian metabolism.

Original languageEnglish (US)
Pages (from-to)2965-2969
Number of pages5
JournalFederation Proceedings
Volume45
Issue number13
StatePublished - Dec 1 1986

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Cholinesterases
Molecular Biology
Oligonucleotide Probes
Serum
Peptides
Amino Acids
Torpedo
Amino Acid Sequence Homology
Genomic Library
Gene Library
Amino Acid Sequence
Fishes
Peptide Hydrolases
Clone Cells
Liver
Enzymes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Molecular biology of human serum cholinesterase. / La Du, B. N.; Lockridge, O.

In: Federation Proceedings, Vol. 45, No. 13, 01.12.1986, p. 2965-2969.

Research output: Contribution to journalReview article

La Du, B. N. ; Lockridge, O. / Molecular biology of human serum cholinesterase. In: Federation Proceedings. 1986 ; Vol. 45, No. 13. pp. 2965-2969.
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