Molecular basis for the auxin-independent phenotype of crown gall tumor tissues

Michael F. Thomashow, Suzanne Hugly, Wallace G Buchholz, Linda S. Thomashow

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

The transfer of specific Ti (tumor-inducing) plasmid sequences, the T-DNA, from Agrobacterium tumefaciens to a wide range of plants results in the formation of crown gall tumors. These tissues differ from most plant cells in that they can be grown in vitro in the absence of added phytohormones. Here, data are presented that offer an explanation for the auxin-independent phenotype of crown gall tissues. It is shown that crude cell-free extracts prepared from three bacterial species harboring pTiA6 gene 1 could convert L-tryptophan to indole-3-acetamide; control extracts lacking gene 1 could not carry out the reaction. Other reports indicate that the pTiA6 gene 2 product can convert indole-3-acetamide to indole-3-acetic acid, a naturally occurring auxin of plants. It is concluded that the auxin-independent phenotype of crown gall tissue involves the introduction of Ti plasmid sequences encoding a two-step pathway for auxin synthesis.

Original languageEnglish (US)
Pages (from-to)616-618
Number of pages3
JournalScience
Volume231
Issue number4738
DOIs
StatePublished - Jan 1 1986

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Plant Tumors
Indoleacetic Acids
Phenotype
Neoplasms
Plasmids
Genes
Agrobacterium tumefaciens
Plant Growth Regulators
Plant Cells
Cell Extracts
Tryptophan

ASJC Scopus subject areas

  • General

Cite this

Molecular basis for the auxin-independent phenotype of crown gall tumor tissues. / Thomashow, Michael F.; Hugly, Suzanne; Buchholz, Wallace G; Thomashow, Linda S.

In: Science, Vol. 231, No. 4738, 01.01.1986, p. 616-618.

Research output: Contribution to journalArticle

Thomashow, Michael F. ; Hugly, Suzanne ; Buchholz, Wallace G ; Thomashow, Linda S. / Molecular basis for the auxin-independent phenotype of crown gall tumor tissues. In: Science. 1986 ; Vol. 231, No. 4738. pp. 616-618.
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