Molecular and physiological evaluation of pancreatic cancer-induced cachexia

Surendra K. Shukla, Aneesha Dasgupta, Scott E. Mulder, Pankaj Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cachexia, a complex metabolic syndrome, is characterized by involuntary weight loss along with muscle wasting and fat depletion leading to poor quality of life of patients. About 80% of pancreatic cancer patients exhibit cachectic phenotype at the time of diagnosis. Here, we present the several molecular and physiological parameters, which we utilize to study the pancreatic cancer-induced cachexia in in vitro models and preclinical mice models of pancreatic cancer. We have described myotube and adipocyte-based in vitro models of muscle and fat wasting, including methods of cell culture, differentiation, and treatment with cancer cell-conditioned medium. Furthermore, we have explained the methods of evaluation of key cachectic markers for muscles. Next, we have detailed the orthotopic implantation mouse models of pancreatic cancer and evaluation of different physiological parameters, including body weight, food intake, body composition analysis, glucose tolerance test, insulin resistance test, grip strength measurement, and rotarod performance test. We have also explained morphological parameters and molecular markers to evaluate the muscle wasting in pancreatic cancer-induced cachexia.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages321-333
Number of pages13
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1882
ISSN (Print)1064-3745

Fingerprint

Cachexia
Pancreatic Neoplasms
Muscles
Fats
Rotarod Performance Test
Skeletal Muscle Fibers
Hand Strength
Glucose Tolerance Test
Conditioned Culture Medium
Body Composition
Adipocytes
Insulin Resistance
Weight Loss
Cell Differentiation
Cell Culture Techniques
Eating
Body Weight
Quality of Life
Phenotype
Neoplasms

Keywords

  • 3T3L1
  • C2C12
  • Cachexia
  • Fat depletion
  • Muscle wasting
  • Orthotopic implantation model
  • Pancreatic cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Shukla, S. K., Dasgupta, A., Mulder, S. E., & Singh, P. (2019). Molecular and physiological evaluation of pancreatic cancer-induced cachexia. In Methods in Molecular Biology (pp. 321-333). (Methods in Molecular Biology; Vol. 1882). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8879-2_28

Molecular and physiological evaluation of pancreatic cancer-induced cachexia. / Shukla, Surendra K.; Dasgupta, Aneesha; Mulder, Scott E.; Singh, Pankaj.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 321-333 (Methods in Molecular Biology; Vol. 1882).

Research output: Chapter in Book/Report/Conference proceedingChapter

Shukla, SK, Dasgupta, A, Mulder, SE & Singh, P 2019, Molecular and physiological evaluation of pancreatic cancer-induced cachexia. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1882, Humana Press Inc., pp. 321-333. https://doi.org/10.1007/978-1-4939-8879-2_28
Shukla SK, Dasgupta A, Mulder SE, Singh P. Molecular and physiological evaluation of pancreatic cancer-induced cachexia. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 321-333. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8879-2_28
Shukla, Surendra K. ; Dasgupta, Aneesha ; Mulder, Scott E. ; Singh, Pankaj. / Molecular and physiological evaluation of pancreatic cancer-induced cachexia. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 321-333 (Methods in Molecular Biology).
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