Abstract
Cachexia, a complex metabolic syndrome, is characterized by involuntary weight loss along with muscle wasting and fat depletion leading to poor quality of life of patients. About 80% of pancreatic cancer patients exhibit cachectic phenotype at the time of diagnosis. Here, we present the several molecular and physiological parameters, which we utilize to study the pancreatic cancer-induced cachexia in in vitro models and preclinical mice models of pancreatic cancer. We have described myotube and adipocyte-based in vitro models of muscle and fat wasting, including methods of cell culture, differentiation, and treatment with cancer cell-conditioned medium. Furthermore, we have explained the methods of evaluation of key cachectic markers for muscles. Next, we have detailed the orthotopic implantation mouse models of pancreatic cancer and evaluation of different physiological parameters, including body weight, food intake, body composition analysis, glucose tolerance test, insulin resistance test, grip strength measurement, and rotarod performance test. We have also explained morphological parameters and molecular markers to evaluate the muscle wasting in pancreatic cancer-induced cachexia.
| Language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 321-333 |
| Number of pages | 13 |
| DOIs | |
| State | Published - Jan 1 2019 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1882 |
| ISSN (Print) | 1064-3745 |
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Keywords
- 3T3L1
- C2C12
- Cachexia
- Fat depletion
- Muscle wasting
- Orthotopic implantation model
- Pancreatic cancer
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Molecular and physiological evaluation of pancreatic cancer-induced cachexia. / Shukla, Surendra K.; Dasgupta, Aneesha; Mulder, Scott E.; Singh, Pankaj.
Methods in Molecular Biology. Humana Press Inc., 2019. p. 321-333 (Methods in Molecular Biology; Vol. 1882).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Molecular and physiological evaluation of pancreatic cancer-induced cachexia
AU - Shukla, Surendra K.
AU - Dasgupta, Aneesha
AU - Mulder, Scott E.
AU - Singh, Pankaj
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Cachexia, a complex metabolic syndrome, is characterized by involuntary weight loss along with muscle wasting and fat depletion leading to poor quality of life of patients. About 80% of pancreatic cancer patients exhibit cachectic phenotype at the time of diagnosis. Here, we present the several molecular and physiological parameters, which we utilize to study the pancreatic cancer-induced cachexia in in vitro models and preclinical mice models of pancreatic cancer. We have described myotube and adipocyte-based in vitro models of muscle and fat wasting, including methods of cell culture, differentiation, and treatment with cancer cell-conditioned medium. Furthermore, we have explained the methods of evaluation of key cachectic markers for muscles. Next, we have detailed the orthotopic implantation mouse models of pancreatic cancer and evaluation of different physiological parameters, including body weight, food intake, body composition analysis, glucose tolerance test, insulin resistance test, grip strength measurement, and rotarod performance test. We have also explained morphological parameters and molecular markers to evaluate the muscle wasting in pancreatic cancer-induced cachexia.
AB - Cachexia, a complex metabolic syndrome, is characterized by involuntary weight loss along with muscle wasting and fat depletion leading to poor quality of life of patients. About 80% of pancreatic cancer patients exhibit cachectic phenotype at the time of diagnosis. Here, we present the several molecular and physiological parameters, which we utilize to study the pancreatic cancer-induced cachexia in in vitro models and preclinical mice models of pancreatic cancer. We have described myotube and adipocyte-based in vitro models of muscle and fat wasting, including methods of cell culture, differentiation, and treatment with cancer cell-conditioned medium. Furthermore, we have explained the methods of evaluation of key cachectic markers for muscles. Next, we have detailed the orthotopic implantation mouse models of pancreatic cancer and evaluation of different physiological parameters, including body weight, food intake, body composition analysis, glucose tolerance test, insulin resistance test, grip strength measurement, and rotarod performance test. We have also explained morphological parameters and molecular markers to evaluate the muscle wasting in pancreatic cancer-induced cachexia.
KW - 3T3L1
KW - C2C12
KW - Cachexia
KW - Fat depletion
KW - Muscle wasting
KW - Orthotopic implantation model
KW - Pancreatic cancer
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UR - http://www.scopus.com/inward/citedby.url?scp=85055656263&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-8879-2_28
DO - 10.1007/978-1-4939-8879-2_28
M3 - Chapter
T3 - Methods in Molecular Biology
SP - 321
EP - 333
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -