Molecular and biochemical characterization of a cytokinin oxidase from maize

K. D. Bilyeu, J. L. Cole, J. G. Laskey, Wayne Riekhof, T. J. Esparza, M. D. Kramer, R. O. Morris

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

It is generally accepted that cytokinin oxidases, which oxidatively remove cytokinin side chains to produce adenine and the corresponding isopentenyl aldehyde, play a major role in regulating cytokinin levels in planta. Partially purified fractions of cytokinin oxidase from various species have been studied for many years, but have yet to clearly reveal the properties of the enzyme or to define its biological significance. Details of the genomic organization of the recently isolated maize (Zea mays) cytokinin oxidase gene (ckx1) and some of its Arabidopsis homologs are now presented. Expression of an intronless ckx1 in Pichia pastoris allowed production of large amounts of recombinant cytokinin oxidase and facilitated detailed kinetic and cofactor analysis and comparison with the native enzyme. The enzyme is a flavoprotein containing covalently bound flavin adenine dinucleotide, but no detectable heavy metals. Expression of the oxidase in maize tissues is described.

Original languageEnglish (US)
Pages (from-to)378-386
Number of pages9
JournalPlant Physiology
Volume125
Issue number1
DOIs
StatePublished - Feb 15 2001

Fingerprint

cytokinins
Zea mays
Cytokinins
corn
Enzymes
Flavoproteins
Flavin-Adenine Dinucleotide
Pichia
Adenine
enzymes
Heavy Metals
Arabidopsis
Aldehydes
flavoproteins
Oxidoreductases
Pichia pastoris
adenine
Plantae
aldehydes
cytokinin oxidase

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Bilyeu, K. D., Cole, J. L., Laskey, J. G., Riekhof, W., Esparza, T. J., Kramer, M. D., & Morris, R. O. (2001). Molecular and biochemical characterization of a cytokinin oxidase from maize. Plant Physiology, 125(1), 378-386. https://doi.org/10.1104/pp.125.1.378

Molecular and biochemical characterization of a cytokinin oxidase from maize. / Bilyeu, K. D.; Cole, J. L.; Laskey, J. G.; Riekhof, Wayne; Esparza, T. J.; Kramer, M. D.; Morris, R. O.

In: Plant Physiology, Vol. 125, No. 1, 15.02.2001, p. 378-386.

Research output: Contribution to journalArticle

Bilyeu, KD, Cole, JL, Laskey, JG, Riekhof, W, Esparza, TJ, Kramer, MD & Morris, RO 2001, 'Molecular and biochemical characterization of a cytokinin oxidase from maize', Plant Physiology, vol. 125, no. 1, pp. 378-386. https://doi.org/10.1104/pp.125.1.378
Bilyeu, K. D. ; Cole, J. L. ; Laskey, J. G. ; Riekhof, Wayne ; Esparza, T. J. ; Kramer, M. D. ; Morris, R. O. / Molecular and biochemical characterization of a cytokinin oxidase from maize. In: Plant Physiology. 2001 ; Vol. 125, No. 1. pp. 378-386.
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