Molecular analysis of the ADH1-Cm allele of maize

John Osterman, Elizabeth S. Dennis

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The Adh1-Cmallele and each gene in the Adh1-FCmduplication have been cloned and restriction-mapped. Of the Cmallele 6 kb was sequenced. A single amino acid substitution of aspartate for tyrosine at residue 52 accounts for the altered enzymatic properties of the Cmprotein. Comparison of the nucleotide sequence to that of Adh1-1F and Adh1-1S shows structural and restriction site polymorphisms in the 3′ flanking DNA. Cmlacks the insertion sequence present in 1F and 1S and contains a complex sequence composed of two direct repeats and an inverted repeat. The two genes of the duplication allele have similar restriction maps to Cmand each other.

Original languageEnglish (US)
Pages (from-to)203-212
Number of pages10
JournalPlant Molecular Biology
Volume13
Issue number2
DOIs
StatePublished - Aug 1 1989

Fingerprint

Gene Duplication
Nucleic Acid Repetitive Sequences
Insertional Mutagenesis
amino acid substitution
aspartic acid
gene duplication
Amino Acid Substitution
Aspartic Acid
transposons
Zea mays
Tyrosine
tyrosine
Genes
Alleles
genetic polymorphism
alleles
nucleotide sequences
corn
DNA
Polymorphism

Keywords

  • alcohol dehydrogenase
  • intragenic recombination
  • restriction site polymorphism

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Molecular analysis of the ADH1-Cm allele of maize. / Osterman, John; Dennis, Elizabeth S.

In: Plant Molecular Biology, Vol. 13, No. 2, 01.08.1989, p. 203-212.

Research output: Contribution to journalArticle

Osterman, John ; Dennis, Elizabeth S. / Molecular analysis of the ADH1-Cm allele of maize. In: Plant Molecular Biology. 1989 ; Vol. 13, No. 2. pp. 203-212.
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