Modulation of p73 isoforms expression induces anti-proliferative and pro-apoptotic activity in mantle cell lymphoma independent of p53 status

Hesham M. Hassan, Michelle L. Varney, Nagendra K. Chaturvedi, Shantaram S Joshi, Dennis D. Weisenburger, Rakesh K Singh, Bhavana J Dave

Research output: Contribution to journalArticle

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Abstract

Mantle cell lymphoma (MCL) is characterized by a clinically aggressive course with frequent relapse and poor survival. The p53 pathway is frequently dysregulated and p53 status predicts clinical outcome. In this report, we investigated whether modulation of p73 isoforms by diclofenac inhibits cell growth, induces apoptosis and/or cell cycle arrest in MCL relative to p53 status. Wild-type p53 [Granta-519 and JVM-2], mutant p53 [Jeko-1 and Mino-1] expressing cells, therapy resistant cell lines, and primary human cells isolated from MCL patients were used. Overexpression of pro-apoptotic TAp73 enhanced MCL cell apoptosis. Diclofenac induced a concentration- and duration-dependent increase in TAp73, cell cycle arrest, cell death, and inhibited MCL cell growth independent of p53 status. Diclofenac treatment was associated with increased activity of caspases 3, 7, and 8 and induction of p53 transcriptional target genes. These studies demonstrate the potential for diclofenac as novel therapeutic agent in MCL independent of p53 status.

Original languageEnglish (US)
Pages (from-to)2874-2889
Number of pages16
JournalLeukemia and Lymphoma
Volume57
Issue number12
DOIs
StatePublished - Dec 1 2016

Fingerprint

Mantle-Cell Lymphoma
Protein Isoforms
Diclofenac
Cell Cycle Checkpoints
Apoptosis
Caspase 7
Caspase 8
Growth
Cell- and Tissue-Based Therapy
Caspase 3
Cell Death
Recurrence
Cell Line
Survival
Therapeutics
Genes

Keywords

  • Apoptosis
  • cell cycle
  • diclofenac
  • mantle cell lymphoma
  • p73 isoforms

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Modulation of p73 isoforms expression induces anti-proliferative and pro-apoptotic activity in mantle cell lymphoma independent of p53 status. / Hassan, Hesham M.; Varney, Michelle L.; Chaturvedi, Nagendra K.; Joshi, Shantaram S; Weisenburger, Dennis D.; Singh, Rakesh K; Dave, Bhavana J.

In: Leukemia and Lymphoma, Vol. 57, No. 12, 01.12.2016, p. 2874-2889.

Research output: Contribution to journalArticle

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AU - Joshi, Shantaram S

AU - Weisenburger, Dennis D.

AU - Singh, Rakesh K

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AB - Mantle cell lymphoma (MCL) is characterized by a clinically aggressive course with frequent relapse and poor survival. The p53 pathway is frequently dysregulated and p53 status predicts clinical outcome. In this report, we investigated whether modulation of p73 isoforms by diclofenac inhibits cell growth, induces apoptosis and/or cell cycle arrest in MCL relative to p53 status. Wild-type p53 [Granta-519 and JVM-2], mutant p53 [Jeko-1 and Mino-1] expressing cells, therapy resistant cell lines, and primary human cells isolated from MCL patients were used. Overexpression of pro-apoptotic TAp73 enhanced MCL cell apoptosis. Diclofenac induced a concentration- and duration-dependent increase in TAp73, cell cycle arrest, cell death, and inhibited MCL cell growth independent of p53 status. Diclofenac treatment was associated with increased activity of caspases 3, 7, and 8 and induction of p53 transcriptional target genes. These studies demonstrate the potential for diclofenac as novel therapeutic agent in MCL independent of p53 status.

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