Modulation of network-driven, GABA-mediated giant depolarizing potentials by SDF-1α in the developing hippocampus

Alexander Kasiyanov, Nobutaka Fujii, Hirokazu Tamamura, Huangui Xiong

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Chemokine stromal cell-derived factor-1 (SDF-1, or CXCL12) plays an important role in brain development and functioning. Whole-cell patch clamp recordings were conducted on CA3 neurons in hippocampal slices prepared from neonatal rats between postnatal days 2 and 6 to study the modulatory effects of SDF-1α on network-driven, γ-aminobutyric-acid-mediated giant depolarizing potentials (GDPs), a hallmark of the developing hippocampus. We found that SDF-1α, the only natural ligand for chemokine CXC motif receptor 4 (CXCR4), decreased GDP firing without significant effects on neuronal passive membrane properties in neonatal hippocampal neurons. The SDF-1α-mediated decrease in GDP firing was blocked by T140, a CXCR4 receptor antagonist, suggesting that SDF-1α modulates GDP firing via CXCR4. We also showed that endogenous SDF-1 exerts a tonic inhibitory action on GDPs in the developing hippocampus. As SDF-1/CXCR4 are highly expressed in the developing brain and GDPs are involved in activity-dependent synapse formation and functioning, the inhibitory action of SDF-1α on GDPs may reflect a potential mechanism for chemokine regulation of neural development in early neonatal life.

Original languageEnglish (US)
Pages (from-to)285-292
Number of pages8
JournalDevelopmental Neuroscience
Volume30
Issue number4
DOIs
StatePublished - May 1 2008

Fingerprint

Chemokines
gamma-Aminobutyric Acid
Hippocampus
CXCR4 Receptors
Aminobutyrates
Neurons
Chemokine CXCL12
Brain
Synapses
Ligands
Membranes
T140 peptide

Keywords

  • Chemokine
  • Chemokine CXC motif receptor 4
  • Hippocampal slices
  • Neurodevelopment
  • Whole-cell recordings

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Modulation of network-driven, GABA-mediated giant depolarizing potentials by SDF-1α in the developing hippocampus. / Kasiyanov, Alexander; Fujii, Nobutaka; Tamamura, Hirokazu; Xiong, Huangui.

In: Developmental Neuroscience, Vol. 30, No. 4, 01.05.2008, p. 285-292.

Research output: Contribution to journalArticle

Kasiyanov, Alexander ; Fujii, Nobutaka ; Tamamura, Hirokazu ; Xiong, Huangui. / Modulation of network-driven, GABA-mediated giant depolarizing potentials by SDF-1α in the developing hippocampus. In: Developmental Neuroscience. 2008 ; Vol. 30, No. 4. pp. 285-292.
@article{f46c527d4d8b4cd687aa4d7ecf455f2f,
title = "Modulation of network-driven, GABA-mediated giant depolarizing potentials by SDF-1α in the developing hippocampus",
abstract = "Chemokine stromal cell-derived factor-1 (SDF-1, or CXCL12) plays an important role in brain development and functioning. Whole-cell patch clamp recordings were conducted on CA3 neurons in hippocampal slices prepared from neonatal rats between postnatal days 2 and 6 to study the modulatory effects of SDF-1α on network-driven, γ-aminobutyric-acid-mediated giant depolarizing potentials (GDPs), a hallmark of the developing hippocampus. We found that SDF-1α, the only natural ligand for chemokine CXC motif receptor 4 (CXCR4), decreased GDP firing without significant effects on neuronal passive membrane properties in neonatal hippocampal neurons. The SDF-1α-mediated decrease in GDP firing was blocked by T140, a CXCR4 receptor antagonist, suggesting that SDF-1α modulates GDP firing via CXCR4. We also showed that endogenous SDF-1 exerts a tonic inhibitory action on GDPs in the developing hippocampus. As SDF-1/CXCR4 are highly expressed in the developing brain and GDPs are involved in activity-dependent synapse formation and functioning, the inhibitory action of SDF-1α on GDPs may reflect a potential mechanism for chemokine regulation of neural development in early neonatal life.",
keywords = "Chemokine, Chemokine CXC motif receptor 4, Hippocampal slices, Neurodevelopment, Whole-cell recordings",
author = "Alexander Kasiyanov and Nobutaka Fujii and Hirokazu Tamamura and Huangui Xiong",
year = "2008",
month = "5",
day = "1",
doi = "10.1159/000112520",
language = "English (US)",
volume = "30",
pages = "285--292",
journal = "Developmental Neuroscience",
issn = "0378-5866",
publisher = "S. Karger AG",
number = "4",

}

TY - JOUR

T1 - Modulation of network-driven, GABA-mediated giant depolarizing potentials by SDF-1α in the developing hippocampus

AU - Kasiyanov, Alexander

AU - Fujii, Nobutaka

AU - Tamamura, Hirokazu

AU - Xiong, Huangui

PY - 2008/5/1

Y1 - 2008/5/1

N2 - Chemokine stromal cell-derived factor-1 (SDF-1, or CXCL12) plays an important role in brain development and functioning. Whole-cell patch clamp recordings were conducted on CA3 neurons in hippocampal slices prepared from neonatal rats between postnatal days 2 and 6 to study the modulatory effects of SDF-1α on network-driven, γ-aminobutyric-acid-mediated giant depolarizing potentials (GDPs), a hallmark of the developing hippocampus. We found that SDF-1α, the only natural ligand for chemokine CXC motif receptor 4 (CXCR4), decreased GDP firing without significant effects on neuronal passive membrane properties in neonatal hippocampal neurons. The SDF-1α-mediated decrease in GDP firing was blocked by T140, a CXCR4 receptor antagonist, suggesting that SDF-1α modulates GDP firing via CXCR4. We also showed that endogenous SDF-1 exerts a tonic inhibitory action on GDPs in the developing hippocampus. As SDF-1/CXCR4 are highly expressed in the developing brain and GDPs are involved in activity-dependent synapse formation and functioning, the inhibitory action of SDF-1α on GDPs may reflect a potential mechanism for chemokine regulation of neural development in early neonatal life.

AB - Chemokine stromal cell-derived factor-1 (SDF-1, or CXCL12) plays an important role in brain development and functioning. Whole-cell patch clamp recordings were conducted on CA3 neurons in hippocampal slices prepared from neonatal rats between postnatal days 2 and 6 to study the modulatory effects of SDF-1α on network-driven, γ-aminobutyric-acid-mediated giant depolarizing potentials (GDPs), a hallmark of the developing hippocampus. We found that SDF-1α, the only natural ligand for chemokine CXC motif receptor 4 (CXCR4), decreased GDP firing without significant effects on neuronal passive membrane properties in neonatal hippocampal neurons. The SDF-1α-mediated decrease in GDP firing was blocked by T140, a CXCR4 receptor antagonist, suggesting that SDF-1α modulates GDP firing via CXCR4. We also showed that endogenous SDF-1 exerts a tonic inhibitory action on GDPs in the developing hippocampus. As SDF-1/CXCR4 are highly expressed in the developing brain and GDPs are involved in activity-dependent synapse formation and functioning, the inhibitory action of SDF-1α on GDPs may reflect a potential mechanism for chemokine regulation of neural development in early neonatal life.

KW - Chemokine

KW - Chemokine CXC motif receptor 4

KW - Hippocampal slices

KW - Neurodevelopment

KW - Whole-cell recordings

UR - http://www.scopus.com/inward/record.url?scp=43249107304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43249107304&partnerID=8YFLogxK

U2 - 10.1159/000112520

DO - 10.1159/000112520

M3 - Article

C2 - 18073458

AN - SCOPUS:43249107304

VL - 30

SP - 285

EP - 292

JO - Developmental Neuroscience

JF - Developmental Neuroscience

SN - 0378-5866

IS - 4

ER -