Modulation of kernel storage proteins in grain sorghum (Sorghum bicolor (L.) Moench)

Tejinder Kumar, Ismail Dweikat, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Han Chen, Thomas E Elthon, Scott Bean, Brian P. Ioerger, Mike Tilley, Tom Clemente

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Sorghum prolamins, termed kafirins, are categorized into subgroups α, β, and γ. The kafirins are co-translationally translocated to the endoplasmic reticulum (ER) where they are assembled into discrete protein bodies that tend to be poorly digestible with low functionality in food and feed applications. As a means to address the issues surrounding functionality and digestibility in sorghum, we employed a biotechnology approach that is designed to alter protein body structure, with the concomitant synthesis of a co-protein in the endosperm fraction of the grain. Wherein perturbation of protein body architecture may provide a route to impact digestibility by reducing disulphide bonds about the periphery of the body, while synthesis of a co-protein, with known functionality attributes, theoretically could impact structure of the protein body through direct association and/or augment end-use applications of sorghum flour by stabilizing ß-sheet formation of the kafirins in sorghum dough preparations. This in turn may improve viscoelasticity of sorghum dough. To this end, we report here on the molecular and phenotypic characterizations of transgenic sorghum events that are down-regulated in γ- and the 29-kDa α-kafirins and the expression of a wheat Dy10/Dx 5 hybrid high-molecular weight glutenin protein. The results demonstrate that down-regulation of γ-kafirin alone does not alter protein body formation or impacts protein digestibility of cooked flour samples. However, reduction in accumulation of a predicted 29-kDa α-kafirin alters the morphology of protein body and enhances protein digestibility in both raw and cooked samples.

Original languageEnglish (US)
Pages (from-to)533-544
Number of pages12
JournalPlant Biotechnology Journal
Volume10
Issue number5
DOIs
StatePublished - Jun 1 2012

Fingerprint

kafirins
protein bodies
Sorghum
grain sorghum
storage proteins
Sorghum bicolor
Sorghum (Poaceae)
seeds
Proteins
digestible protein
dough
digestibility
sorghum flour
viscoelasticity
prolamins
synthesis
Flour
proteins
disulfide bonds
glutenins

Keywords

  • Agrobacterium tumefaciens
  • Digestibility
  • Kafirin
  • Maize
  • Sorghum
  • Transgenic
  • Wheat

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Kumar, T., Dweikat, I., Sato, S., Ge, Z., Nersesian, N., Chen, H., ... Clemente, T. (2012). Modulation of kernel storage proteins in grain sorghum (Sorghum bicolor (L.) Moench). Plant Biotechnology Journal, 10(5), 533-544. https://doi.org/10.1111/j.1467-7652.2012.00685.x

Modulation of kernel storage proteins in grain sorghum (Sorghum bicolor (L.) Moench). / Kumar, Tejinder; Dweikat, Ismail; Sato, Shirley; Ge, Zhengxiang; Nersesian, Natalya; Chen, Han; Elthon, Thomas E; Bean, Scott; Ioerger, Brian P.; Tilley, Mike; Clemente, Tom.

In: Plant Biotechnology Journal, Vol. 10, No. 5, 01.06.2012, p. 533-544.

Research output: Contribution to journalArticle

Kumar, T, Dweikat, I, Sato, S, Ge, Z, Nersesian, N, Chen, H, Elthon, TE, Bean, S, Ioerger, BP, Tilley, M & Clemente, T 2012, 'Modulation of kernel storage proteins in grain sorghum (Sorghum bicolor (L.) Moench)', Plant Biotechnology Journal, vol. 10, no. 5, pp. 533-544. https://doi.org/10.1111/j.1467-7652.2012.00685.x
Kumar, Tejinder ; Dweikat, Ismail ; Sato, Shirley ; Ge, Zhengxiang ; Nersesian, Natalya ; Chen, Han ; Elthon, Thomas E ; Bean, Scott ; Ioerger, Brian P. ; Tilley, Mike ; Clemente, Tom. / Modulation of kernel storage proteins in grain sorghum (Sorghum bicolor (L.) Moench). In: Plant Biotechnology Journal. 2012 ; Vol. 10, No. 5. pp. 533-544.
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