Modulation of GSH with exogenous agents leads to changes in glyoxalase 1 enzyme activity in VL-17A cells exposed to chronic alcohol plus high glucose

S. Mathan Kumar, Kavitha Swaminathan, Dahn L Clemens, Aparajita Dey

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Gluthathione (GSH) is a major cellular antioxidant. The present study utilizing VL-17A cells exposed to chronic alcohol plus high glucose investigated the changes in oxidative stress, toxicity, and glyoxalase 1 activity as a detoxification pathway due to changes in GSH level through GSH supplementation with N-acetyl cysteine (NAC) or ursodeoxycholic acid (UDCA) and its depletion through buthionine sulfoximine (BSO) or diethyl maleate (DEM). Glyoxalase 1 plays an important role in detoxification of methylglyoxal which is formed as a precursor of advanced glycated end products formed due to high glucose mediated oxidative stress. Significant changes in glyoxalase 1 activity utilizing methylglyoxal or glyoxal as substrates occurred with NAC or UDCA or BSO or DEM supplementation in chronic alcohol plus high glucose treated VL-17A cells. NAC or UDCA administration in chronic alcohol plus high glucose treated VL-17A cells increased viability and decreased ROS levels, lipid peroxidation and 3-nitrotyrosine adduct formation. Similarly, GSH depletion with BSO or DEM had an opposite effect on the parameters in chronic alcohol plus high glucose treated VL-17A cells. In conclusion, modulation of GSH with NAC or UDCA or BSO or DEM leads to significant changes in oxidative stress, glyoxalase 1 enzyme activity and toxicity in chronic alcohol plus high glucose treated VL-17A cells.

Original languageEnglish (US)
Pages (from-to)345-358
Number of pages14
JournalFood and Function
Volume5
Issue number2
DOIs
StatePublished - Feb 1 2014

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diethyl maleate
ursodeoxycholic acid
Buthionine Sulfoximine
maleates
Ursodeoxycholic Acid
acetylcysteine
alcohols
Alcohols
enzyme activity
Glucose
glucose
Cysteine
Enzymes
Pyruvaldehyde
Oxidative Stress
oxidative stress
cells
Glyoxal
chronic toxicity
Lipid Peroxidation

ASJC Scopus subject areas

  • Food Science

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Modulation of GSH with exogenous agents leads to changes in glyoxalase 1 enzyme activity in VL-17A cells exposed to chronic alcohol plus high glucose. / Kumar, S. Mathan; Swaminathan, Kavitha; Clemens, Dahn L; Dey, Aparajita.

In: Food and Function, Vol. 5, No. 2, 01.02.2014, p. 345-358.

Research output: Contribution to journalArticle

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