Modulation of fibronectin production of bovine bronchial epithelial cells by transforming growth factor-beta.

Debra Romberger, J. D. Beckmann, L. Claassen, R. F. Ertl, S. I. Rennard

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Regulation of airway repair after injury is poorly understood but is thought to be important in the development of airway diseases such as chronic bronchitis and asthma. There is evidence that fibronectin (Fn), an extracellular matrix glycoprotein, has a role in repair processes. In addition, transforming growth factor-beta (TGF-beta) is also likely involved in would healing and is known to influence extracellular matrix constituents in other cell systems. We postulated that TGF-beta may effect airway repair by modulating Fn production from airway epithelial cells. To examine this hypothesis, we studied the effect of TGF-beta 1 on Fn production by bovine bronchial epithelial cells in culture. Fn, released into the media of cultures exposed to TGF-beta 1, increased in a dose- and time-responsive fashion. Fn in the cell layer also increased in response to TGF-beta 1. De novo protein synthesis was demonstrated by an increase in [35S]methionine incorporation into Fn immunoprecipitated from media of TGF-beta-treated cultures. TGF-beta 1 also induced an increase in expression of Fn mRNA from cultured bronchial epithelial cells, suggesting that TGF-beta modulates Fn production of these cells, at least in part, through modulation of Fn gene expression. These data support a role for TGF-beta in airway repair through modulation of Fn production by airway epithelium.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalAmerican journal of respiratory cell and molecular biology
Volume7
Issue number2
DOIs
StatePublished - Aug 1992

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Fibronectins
Transforming Growth Factor beta
Epithelial Cells
Modulation
Repair
Extracellular Matrix
Chronic Bronchitis
Gene expression
Methionine
Culture Media
Glycoproteins
Asthma
Epithelium
Cell Culture Techniques
Cells
Gene Expression
Messenger RNA
Wounds and Injuries

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Modulation of fibronectin production of bovine bronchial epithelial cells by transforming growth factor-beta. / Romberger, Debra; Beckmann, J. D.; Claassen, L.; Ertl, R. F.; Rennard, S. I.

In: American journal of respiratory cell and molecular biology, Vol. 7, No. 2, 08.1992, p. 149-155.

Research output: Contribution to journalArticle

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