Modulation of alveolar macrophage leukotrien B4 released by complement component C5

R. A. Robbins, G. L. Gossman, L. A. Allington, T. J. Kendall, T. W. Stull, Stephen Israel Rennard

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The release of neutrophil chemotactic activity by the guinea pig alveolar macrophage (AM) is dependent on the fifth component of complement (C5) on the cell surface. Because one potent chemotactic factor released by AMs is leukotriene B4 (LTB4), we hypothesized that cell surface C5 may modulate LTB4 release. To test this hypothesis, human AMs obtained by bronchoalveolar lavage from 12 subjects were cultured for 4 hours in the presence of anti-C5 Fab' antibodies with stimuli. The cultures were harvested and evaluated for LTB4 by radioimmunoassay. The LTB4 levels in supernatants obtained from AMs cultured in media alone were variable (447 ± 63 pg/ml), but the levels were increased when AMs when cultured with the stimuli-opsonized zymosan, immune complexes, or lipopolysaccharide (233%, 49%, and 114% increase, respectively, compared with macrophages cultured in media alone, p < 0.05). Culturing the AMs with anti-C5 Fab' antibodies inhibited the release of LTB4 induced by opsonized zymosan, immune complexes, or lipopolysaccharide (78%, 41%, and 82% inhibition, respectively, p < 0.05). Consistent with these observations, anti-C5 Fab' antibodies also decreased the neutrophil chemotactic activity of culture supernatants obtained from AMs stimulated with the same stimuli (P < 0.001). These data suggest that AM release of LTB4 may be C5-dependent.

Original languageEnglish (US)
Pages (from-to)497-503
Number of pages7
JournalJournal of Laboratory and Clinical Medicine
Volume115
Issue number4
StatePublished - Jan 1 1990

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Complement C5
Leukotriene B4
Alveolar Macrophages
Modulation
Zymosan
Antigen-Antibody Complex
Lipopolysaccharides
Antibodies
Neutrophils
Macrophages
Chemotactic Factors
Bronchoalveolar Lavage
Radioimmunoassay
Guinea Pigs

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Robbins, R. A., Gossman, G. L., Allington, L. A., Kendall, T. J., Stull, T. W., & Rennard, S. I. (1990). Modulation of alveolar macrophage leukotrien B4 released by complement component C5. Journal of Laboratory and Clinical Medicine, 115(4), 497-503.

Modulation of alveolar macrophage leukotrien B4 released by complement component C5. / Robbins, R. A.; Gossman, G. L.; Allington, L. A.; Kendall, T. J.; Stull, T. W.; Rennard, Stephen Israel.

In: Journal of Laboratory and Clinical Medicine, Vol. 115, No. 4, 01.01.1990, p. 497-503.

Research output: Contribution to journalArticle

Robbins, RA, Gossman, GL, Allington, LA, Kendall, TJ, Stull, TW & Rennard, SI 1990, 'Modulation of alveolar macrophage leukotrien B4 released by complement component C5', Journal of Laboratory and Clinical Medicine, vol. 115, no. 4, pp. 497-503.
Robbins RA, Gossman GL, Allington LA, Kendall TJ, Stull TW, Rennard SI. Modulation of alveolar macrophage leukotrien B4 released by complement component C5. Journal of Laboratory and Clinical Medicine. 1990 Jan 1;115(4):497-503.
Robbins, R. A. ; Gossman, G. L. ; Allington, L. A. ; Kendall, T. J. ; Stull, T. W. ; Rennard, Stephen Israel. / Modulation of alveolar macrophage leukotrien B4 released by complement component C5. In: Journal of Laboratory and Clinical Medicine. 1990 ; Vol. 115, No. 4. pp. 497-503.
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