Modulation of a high molecular weight form of transforming growth factor-α in human colon carcinoma cell lines

L. F. Watkins, M. G. Brattain, A. E. Levine

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The secretion of transforming growth factor-α (TGF-α) by several human colon carcinoma cell lines in tissue culture medium was examined. All of the cell lines tested secreted TGF-α to varying degrees. Bio-Gel P-30 chromatography of the conditioned media from three of these cell lines (HCT 116, MOSER, FET) indicated differences in the molecular weights of secreted TGF-α. In the HCT 116 cell line, the majority of the TGF-like activity had a molecular weight of less than 10,000. For both MOSER and FET cell lines, 20-30% of the TGF-like activity had a molecular weight greater than 15,000. When HCT 116 and MOSER cells were treated with differentiation inducing agents there was an increase in a TGF-α species whose molecular weight was greater than 20,000. This indicates a possible alteration in either the processing of the TGF-α precursor and/or secretion of precursor products by the different cell lines.

Original languageEnglish (US)
Pages (from-to)59-70
Number of pages12
JournalCancer Letters
Volume40
Issue number1
DOIs
StatePublished - May 1988

Fingerprint

Transforming Growth Factors
Colon
Molecular Weight
Carcinoma
Cell Line
HCT116 Cells
Conditioned Culture Medium
Culture Media
Chromatography
Gels

Keywords

  • Colon cancer
  • Differentiation
  • Transforming growth factor-α

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Modulation of a high molecular weight form of transforming growth factor-α in human colon carcinoma cell lines. / Watkins, L. F.; Brattain, M. G.; Levine, A. E.

In: Cancer Letters, Vol. 40, No. 1, 05.1988, p. 59-70.

Research output: Contribution to journalArticle

Watkins, L. F. ; Brattain, M. G. ; Levine, A. E. / Modulation of a high molecular weight form of transforming growth factor-α in human colon carcinoma cell lines. In: Cancer Letters. 1988 ; Vol. 40, No. 1. pp. 59-70.
@article{6c19c3a912394fb6844a94a72ff41e57,
title = "Modulation of a high molecular weight form of transforming growth factor-α in human colon carcinoma cell lines",
abstract = "The secretion of transforming growth factor-α (TGF-α) by several human colon carcinoma cell lines in tissue culture medium was examined. All of the cell lines tested secreted TGF-α to varying degrees. Bio-Gel P-30 chromatography of the conditioned media from three of these cell lines (HCT 116, MOSER, FET) indicated differences in the molecular weights of secreted TGF-α. In the HCT 116 cell line, the majority of the TGF-like activity had a molecular weight of less than 10,000. For both MOSER and FET cell lines, 20-30{\%} of the TGF-like activity had a molecular weight greater than 15,000. When HCT 116 and MOSER cells were treated with differentiation inducing agents there was an increase in a TGF-α species whose molecular weight was greater than 20,000. This indicates a possible alteration in either the processing of the TGF-α precursor and/or secretion of precursor products by the different cell lines.",
keywords = "Colon cancer, Differentiation, Transforming growth factor-α",
author = "Watkins, {L. F.} and Brattain, {M. G.} and Levine, {A. E.}",
year = "1988",
month = "5",
doi = "10.1016/0304-3835(88)90262-5",
language = "English (US)",
volume = "40",
pages = "59--70",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Modulation of a high molecular weight form of transforming growth factor-α in human colon carcinoma cell lines

AU - Watkins, L. F.

AU - Brattain, M. G.

AU - Levine, A. E.

PY - 1988/5

Y1 - 1988/5

N2 - The secretion of transforming growth factor-α (TGF-α) by several human colon carcinoma cell lines in tissue culture medium was examined. All of the cell lines tested secreted TGF-α to varying degrees. Bio-Gel P-30 chromatography of the conditioned media from three of these cell lines (HCT 116, MOSER, FET) indicated differences in the molecular weights of secreted TGF-α. In the HCT 116 cell line, the majority of the TGF-like activity had a molecular weight of less than 10,000. For both MOSER and FET cell lines, 20-30% of the TGF-like activity had a molecular weight greater than 15,000. When HCT 116 and MOSER cells were treated with differentiation inducing agents there was an increase in a TGF-α species whose molecular weight was greater than 20,000. This indicates a possible alteration in either the processing of the TGF-α precursor and/or secretion of precursor products by the different cell lines.

AB - The secretion of transforming growth factor-α (TGF-α) by several human colon carcinoma cell lines in tissue culture medium was examined. All of the cell lines tested secreted TGF-α to varying degrees. Bio-Gel P-30 chromatography of the conditioned media from three of these cell lines (HCT 116, MOSER, FET) indicated differences in the molecular weights of secreted TGF-α. In the HCT 116 cell line, the majority of the TGF-like activity had a molecular weight of less than 10,000. For both MOSER and FET cell lines, 20-30% of the TGF-like activity had a molecular weight greater than 15,000. When HCT 116 and MOSER cells were treated with differentiation inducing agents there was an increase in a TGF-α species whose molecular weight was greater than 20,000. This indicates a possible alteration in either the processing of the TGF-α precursor and/or secretion of precursor products by the different cell lines.

KW - Colon cancer

KW - Differentiation

KW - Transforming growth factor-α

UR - http://www.scopus.com/inward/record.url?scp=0023913144&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023913144&partnerID=8YFLogxK

U2 - 10.1016/0304-3835(88)90262-5

DO - 10.1016/0304-3835(88)90262-5

M3 - Article

C2 - 3259465

AN - SCOPUS:0023913144

VL - 40

SP - 59

EP - 70

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -