Modification of blood group A antigen expression in a pancreatic cancer cell line (PC‐1) by inhibitors of N‐glycan processing

Masahiko Hirota, Masatoshi Mogaki, Parviz M. Pour, William G. Chaney

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Pancreatic adenocarcinomas induced in Syrian hamsters by treatment with N‐nitrosobis(2‐oxopropyl) amine express blood group A antigen, which is absent in normal pancreatic cells. On membrane glycoproteins purified from tumors, blood group A antigen has been found to be expressed on multiantennary Asn‐linked complex glycans. In this study, we investigated the effect of inhibitors of Asn‐glycan processing on blood group A antigen bearing glycan structures in a cell line (PC‐1) established from a primary induced pancreatic cancer. Expression of blood group A antigen on cells and in membrane preparations was blocked by treatment with 1‐deoxymannojirimycin, an inhibitor of mannosidase I, but was retained after treatment with swainsonine, an inhibitor of mannosidase II. However, swainsonine treatment altered the glycan structure associated with blood group A antigen from an endoglycosidase H resistant type to a sensitive type, indicating that the blood group A structure might shift from a complex type to a hybr‐id type glycan by this treatment. These results demonstrate that Asn‐linked glycans carry the major blood group A antigens in PC‐1 cells. © 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)13-20
Number of pages8
JournalJournal of Cellular Biochemistry
Volume50
Issue number1
DOIs
Publication statusPublished - Sep 1992

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Keywords

  • 1‐deoxymannojirimycin
  • Asn‐linked glycan
  • blood group A antigen
  • pancreatic cancer cell line
  • swainsonine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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