Moderation of hyperuricemia in rats: Via consuming walnut protein hydrolysate diet and identification of new antihyperuricemic peptides

Qingyong Li, Xiaoyan Kang, Chuanchao Shi, Yujuan Li, Kaustav Majumder, Zhengxiang Ning, Jiaoyan Ren

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, walnut meal hydrolysates (WMH) and dephenolized walnut meal hydrolysates (DWMH) were found to effectively decrease the serum uric acid level and protect the renal function in potassium oxonate-induced hyperuricemic rats in vivo as well as inhibit xanthine oxidase in vitro. Two novel antihyperuricemic peptides including WPPKN (640.8 Da) and ADIYTE (710.7 Da) were purified from DWMH via Sephadex G-15 gel filtration and reverse-phase high-performance liquid chromatography and identified by LC-ESI-MS/MS. These peptides displayed high in vitro xanthine oxidase inhibition (XOI) activity with IC 50 values of 17.75 ± 0.12 mg mL -1 (WPPKN) and 19.01 ± 0.23 mg mL -1 (ADIYTE). Based on the results of molecular simulation, WPPKN entered into the hydrophobic channel and even obstructed the interaction between xanthine and xanthine oxidase (XO), while ADIYTE was positioned on the surface of the B-chain and blocked the entrance of the substrate to the hydrophobic channel. Therefore, the two peptides are partiqlly responsible for the antihyperuricemic properties of DWMH.

Original languageEnglish (US)
Pages (from-to)107-116
Number of pages10
JournalFood and Function
Volume9
Issue number1
DOIs
StatePublished - Jan 2018

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Gout Suppressants
hyperuricemia
Juglans
Protein Hydrolysates
Hyperuricemia
protein hydrolysates
walnuts
hydrolysates
xanthine oxidase
Meals
Xanthine Oxidase
peptides
Diet
Peptides
rats
diet
xanthine
Xanthine
reversed-phase liquid chromatography
renal function

ASJC Scopus subject areas

  • Food Science

Cite this

Moderation of hyperuricemia in rats : Via consuming walnut protein hydrolysate diet and identification of new antihyperuricemic peptides. / Li, Qingyong; Kang, Xiaoyan; Shi, Chuanchao; Li, Yujuan; Majumder, Kaustav; Ning, Zhengxiang; Ren, Jiaoyan.

In: Food and Function, Vol. 9, No. 1, 01.2018, p. 107-116.

Research output: Contribution to journalArticle

Li, Qingyong ; Kang, Xiaoyan ; Shi, Chuanchao ; Li, Yujuan ; Majumder, Kaustav ; Ning, Zhengxiang ; Ren, Jiaoyan. / Moderation of hyperuricemia in rats : Via consuming walnut protein hydrolysate diet and identification of new antihyperuricemic peptides. In: Food and Function. 2018 ; Vol. 9, No. 1. pp. 107-116.
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