Mitochondrial morphology and dynamics in hepatocytes from normal and ethanol-fed rats

Sudipto Das, Nora Hajnóczky, Anil Noronha Antony, György Csordás, Lawrence D. Gaspers, Dahn L. Clemens, Jan B. Hoek, György Hajnóczky

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Mitochondrial structure and function are central to cell physiology and are mutually interdependent. Mitochondria represent a primary target of the alcohol-induced tissue injury, particularly in the liver, where the metabolic effects of ethanol are predominant. However, the effect of ethanol on hepatic mitochondrial morphology and dynamics remain to be established. In the present work, we employed the organelle-targeted photoactivatable fluorescent protein technology and electron microscopy to study hepatic mitochondrial structure and dynamics. Hepatocytes in perfused liver as well as in primary cultures showed mostly discrete globular or short tubular mitochondria. The mitochondria showed few fusion events and little movement activity. By contrast, human hepatoma (HepG2)-derived VL-17A cells, expressing the major hepatic ethanol metabolizing enzymes, alcohol dehydrogenase and cytochrome P450 2E1, have elongated and interconnected mitochondria showing matrix continuity and many fusion events. Hepatocytes isolated from chronically ethanol-fed rats showed some increase in mitochondrial volume and exhibited a substantial suppression of mitochondrial dynamics. In VL-17A cells, prolonged ethanol exposure also caused decreased mitochondrial continuity and dynamics. Collectively, these results indicate that mitochondria in normal hepatocytes show relatively slow dynamics, which is very sensitive to suppression by ethanol exposure.

Original languageEnglish (US)
Pages (from-to)101-109
Number of pages9
JournalPflugers Archiv European Journal of Physiology
Volume464
Issue number1
DOIs
StatePublished - Jul 1 2012

Fingerprint

Mitochondrial Dynamics
Mitochondria
Rats
Hepatocytes
Ethanol
Liver
Fusion reactions
Mitochondrial Size
Cell Physiological Phenomena
Cytochrome P-450 CYP2E1
Alcohol Dehydrogenase
Physiology
Cell culture
Organelles
Electron microscopy
Hepatocellular Carcinoma
Electron Microscopy
Alcohols
Tissue
Technology

Keywords

  • Alcohol
  • Alcoholic liver disease
  • Fusion
  • Mitochondria
  • Motility
  • Oxidative stress

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

Mitochondrial morphology and dynamics in hepatocytes from normal and ethanol-fed rats. / Das, Sudipto; Hajnóczky, Nora; Antony, Anil Noronha; Csordás, György; Gaspers, Lawrence D.; Clemens, Dahn L.; Hoek, Jan B.; Hajnóczky, György.

In: Pflugers Archiv European Journal of Physiology, Vol. 464, No. 1, 01.07.2012, p. 101-109.

Research output: Contribution to journalArticle

Das, S, Hajnóczky, N, Antony, AN, Csordás, G, Gaspers, LD, Clemens, DL, Hoek, JB & Hajnóczky, G 2012, 'Mitochondrial morphology and dynamics in hepatocytes from normal and ethanol-fed rats', Pflugers Archiv European Journal of Physiology, vol. 464, no. 1, pp. 101-109. https://doi.org/10.1007/s00424-012-1100-4
Das, Sudipto ; Hajnóczky, Nora ; Antony, Anil Noronha ; Csordás, György ; Gaspers, Lawrence D. ; Clemens, Dahn L. ; Hoek, Jan B. ; Hajnóczky, György. / Mitochondrial morphology and dynamics in hepatocytes from normal and ethanol-fed rats. In: Pflugers Archiv European Journal of Physiology. 2012 ; Vol. 464, No. 1. pp. 101-109.
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