Mitochondrial malate dehydrogenase from corn purification of multiple forms

Marianne K. Hayes, Michael H. Luethy, Thomas E. Elthon

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A method to ractionate corn (Zea mays L. B73) mitochondria into soluble proteins, high molecular weight soluble proteins, and membrane proteins was developed. These fractions were analyzed by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and assays of mitochondria) enzyme activities. The Krebs cycle enzymes were enriched in the soluble fraction. Malate dehydrogenase has been purified from the soluble fraction by a two-step fast protein liquid chromatography method. Six different malate dehydrogenase peaks were obtained from the Mono Q column. These peaks were individually purified using a Phenyl Superose column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified peaks showed that three of the isoenzymes consisted of different homodimers (I, III, VI) and three were different heterodimers (II, IV, V). Apparent molecular masses of the three different monomer subunits were 37, 38, and 39 kilodaltons. Nondenaturing gel analysis of the malate dehydrogenase peaks showed that each Mono Q peak contained a band of malate dehydrogenase activity with different mobility. These observations are consistent with three nuclear genes encoding corn mitochondrial malate dehydrogenase. Polyclonal antibodies raised against purified malate dehydrogenase were used to identify the gene products using Western blots of two-dimensional gels.

Original languageEnglish (US)
Pages (from-to)1381-1387
Number of pages7
JournalPlant physiology
Volume97
Issue number4
DOIs
StatePublished - Jan 1 1991

Fingerprint

Malate Dehydrogenase
malate dehydrogenase
Zea mays
corn
Sodium Dodecyl Sulfate
polyacrylamide gel electrophoresis
Polyacrylamide Gel Electrophoresis
Mitochondria
mitochondria
Gels
gels
molecular weight
Proteins
Citric Acid Cycle
proteins
tricarboxylic acid cycle
Enzyme Assays
polyclonal antibodies
Liquid Chromatography
membrane proteins

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Mitochondrial malate dehydrogenase from corn purification of multiple forms. / Hayes, Marianne K.; Luethy, Michael H.; Elthon, Thomas E.

In: Plant physiology, Vol. 97, No. 4, 01.01.1991, p. 1381-1387.

Research output: Contribution to journalArticle

Hayes, Marianne K. ; Luethy, Michael H. ; Elthon, Thomas E. / Mitochondrial malate dehydrogenase from corn purification of multiple forms. In: Plant physiology. 1991 ; Vol. 97, No. 4. pp. 1381-1387.
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