Mitochondrial DNA Sequences of Triatomines (Hemiptera

Reduviidae): Phylogenetic Relationships

Beatriz A. García, Etsuko Moriyama, Jeffrey R. Powell

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The phylogenetic relationships among 18 species of Triatominae were inferred based on mitochondrial DNA (mtDNA) sequences. The species of Triatoma included 11 belonging to the infestans complex [T. infestans (Klug), T guasayana Wygodzinsky & Abalos, T. sordida (Stål), T. platensis Neiva, T. brasiliensis Neisa, T. rubrovaria (Blanchard), T. vitticeps (Stål), T. delpontei Romaña & Abalos, T. maculata (Erichson), T. patagonica Del Ponte, and T. matogrossensis Leite & Barbosa] and four others of the same genus but of different complexes [T. circummaculata (Stål), T. protract a (Uhler), T. dimidiata (Latreille), and T. mazzuttii Usinger]. As possible outgroups we used Mepraia spinolai Mazza, Panstrongylus megistus (Burmeister), and Rhodnius prolixus Stål. We analyzed mtDNA fragments of the 12S and 16S ribosomal RNA genes from each of the 18 species, as well as of the cytochrome oxidase I (COI) gene from nine. The 12S, 16S, and COI gene sequences were analyzed individually and combined. All of the phylogenetic analyses unambiguously supported two clusters: one including T. infestans, T. platensis, and T. delpontei, and the other T. sordida and T. matogrossensis. Inclusion of T. circummaculata into the infestons complex was confirmed, although this is in disagreement with the morphological classification. On the other hand, our analyses showed that T. dimidiata is closely related to a phyllosoma complex species, T. mazzottii. This is consistent with the tentative classification previously made based on morphological characters. The issue of the monophyly of the genus Triatoma remains unresolved.

Original languageEnglish (US)
Pages (from-to)675-683
Number of pages9
JournalJournal of medical entomology
Volume38
Issue number5
DOIs
StatePublished - Jan 1 2001

Fingerprint

Reduviidae
Triatoma
Triatominae
Hemiptera
Electron Transport Complex IV
Mitochondrial DNA
cytochrome-c oxidase
Triatoma sordida
Panstrongylus megistus
Panstrongylus
mitochondrial DNA
Rhodnius
16S Ribosomal RNA
Triatoma infestans
nucleotide sequences
Rhodnius prolixus
phylogeny
rRNA Genes
monophyly
Genes

Keywords

  • 12S
  • 16S
  • COI
  • Phylogeny
  • Triatominae
  • mtDNA sequences

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Mitochondrial DNA Sequences of Triatomines (Hemiptera : Reduviidae): Phylogenetic Relationships. / García, Beatriz A.; Moriyama, Etsuko; Powell, Jeffrey R.

In: Journal of medical entomology, Vol. 38, No. 5, 01.01.2001, p. 675-683.

Research output: Contribution to journalArticle

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abstract = "The phylogenetic relationships among 18 species of Triatominae were inferred based on mitochondrial DNA (mtDNA) sequences. The species of Triatoma included 11 belonging to the infestans complex [T. infestans (Klug), T guasayana Wygodzinsky & Abalos, T. sordida (St{\aa}l), T. platensis Neiva, T. brasiliensis Neisa, T. rubrovaria (Blanchard), T. vitticeps (St{\aa}l), T. delpontei Roma{\~n}a & Abalos, T. maculata (Erichson), T. patagonica Del Ponte, and T. matogrossensis Leite & Barbosa] and four others of the same genus but of different complexes [T. circummaculata (St{\aa}l), T. protract a (Uhler), T. dimidiata (Latreille), and T. mazzuttii Usinger]. As possible outgroups we used Mepraia spinolai Mazza, Panstrongylus megistus (Burmeister), and Rhodnius prolixus St{\aa}l. We analyzed mtDNA fragments of the 12S and 16S ribosomal RNA genes from each of the 18 species, as well as of the cytochrome oxidase I (COI) gene from nine. The 12S, 16S, and COI gene sequences were analyzed individually and combined. All of the phylogenetic analyses unambiguously supported two clusters: one including T. infestans, T. platensis, and T. delpontei, and the other T. sordida and T. matogrossensis. Inclusion of T. circummaculata into the infestons complex was confirmed, although this is in disagreement with the morphological classification. On the other hand, our analyses showed that T. dimidiata is closely related to a phyllosoma complex species, T. mazzottii. This is consistent with the tentative classification previously made based on morphological characters. The issue of the monophyly of the genus Triatoma remains unresolved.",
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