Mitochondrial DMA polymorphism in a black fly, Simulium vittatum (Diptera

Simuliidae)

X. Zhu, K. P. Pruess, Thomas O Powers

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Mitochondrial DNA (mtDN A) was extracted from pooled field-collected samples representing six species of black flies (Cnephia dacotensis, Simulium bivittaum, S. johansenni, S. luggeri, S. piperi, S. vittatum) and compared by restriction fragment length polymorphism (RFLP) analysis. Morphospecies were molecularly distinct, with few shared restriction fragments. Eleven populations of S. vittatum were found that appeared to be homogeneous for a single mitochondrial haplotype. Ten other populations of S. vittatum showed extensive mitochondrial heterogeneity. In part, these samples contained mixtures of two cytologically recognized siblings: IIIL-1 and IS-7. About 70% of the mitochondrial genome of a population pure for sibling IIIL-1 was cloned as five Hind III fragments, which were used as hybridization probes to examine individual black flies. Thirteen mtDNA haplotypes involving permutations of 10 Hind III restriction sites were identified in individual black flies examined from 26 populations. DNA from 168 larvae cut with both EcoRl and Hind III revealed five additional haplotypes. One Hind III haplotype was present in 84% of 390 larvae examined and predominated in every population examined from New York to California and in both the IIIL-1 and IS-7 siblings. Nebraska populations had individuals with nearly all known haplotypes. The most common haplotype was usually the only form present in warm, silty streams with organic enrichment. Rarer haplotypes were found in cool, spring-fed streams but without clear geographic or phylogenetic components.

Original languageEnglish (US)
Pages (from-to)440-447
Number of pages8
JournalCanadian Journal of Zoology
Volume76
Issue number3
StatePublished - Dec 1 1998

Fingerprint

Simulium vittatum
Simuliidae
haplotypes
polymorphism
genetic polymorphism
larva
mitochondrial DNA
genome
probe
Cnephia
phylogenetics
DNA
Simulium
larvae
restriction fragment length polymorphism
sampling
phylogeny

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology

Cite this

Mitochondrial DMA polymorphism in a black fly, Simulium vittatum (Diptera : Simuliidae). / Zhu, X.; Pruess, K. P.; Powers, Thomas O.

In: Canadian Journal of Zoology, Vol. 76, No. 3, 01.12.1998, p. 440-447.

Research output: Contribution to journalArticle

@article{96d7990f461f42088761df5b2964d591,
title = "Mitochondrial DMA polymorphism in a black fly, Simulium vittatum (Diptera: Simuliidae)",
abstract = "Mitochondrial DNA (mtDN A) was extracted from pooled field-collected samples representing six species of black flies (Cnephia dacotensis, Simulium bivittaum, S. johansenni, S. luggeri, S. piperi, S. vittatum) and compared by restriction fragment length polymorphism (RFLP) analysis. Morphospecies were molecularly distinct, with few shared restriction fragments. Eleven populations of S. vittatum were found that appeared to be homogeneous for a single mitochondrial haplotype. Ten other populations of S. vittatum showed extensive mitochondrial heterogeneity. In part, these samples contained mixtures of two cytologically recognized siblings: IIIL-1 and IS-7. About 70{\%} of the mitochondrial genome of a population pure for sibling IIIL-1 was cloned as five Hind III fragments, which were used as hybridization probes to examine individual black flies. Thirteen mtDNA haplotypes involving permutations of 10 Hind III restriction sites were identified in individual black flies examined from 26 populations. DNA from 168 larvae cut with both EcoRl and Hind III revealed five additional haplotypes. One Hind III haplotype was present in 84{\%} of 390 larvae examined and predominated in every population examined from New York to California and in both the IIIL-1 and IS-7 siblings. Nebraska populations had individuals with nearly all known haplotypes. The most common haplotype was usually the only form present in warm, silty streams with organic enrichment. Rarer haplotypes were found in cool, spring-fed streams but without clear geographic or phylogenetic components.",
author = "X. Zhu and Pruess, {K. P.} and Powers, {Thomas O}",
year = "1998",
month = "12",
day = "1",
language = "English (US)",
volume = "76",
pages = "440--447",
journal = "Canadian Journal of Zoology",
issn = "0008-4301",
publisher = "National Research Council of Canada",
number = "3",

}

TY - JOUR

T1 - Mitochondrial DMA polymorphism in a black fly, Simulium vittatum (Diptera

T2 - Simuliidae)

AU - Zhu, X.

AU - Pruess, K. P.

AU - Powers, Thomas O

PY - 1998/12/1

Y1 - 1998/12/1

N2 - Mitochondrial DNA (mtDN A) was extracted from pooled field-collected samples representing six species of black flies (Cnephia dacotensis, Simulium bivittaum, S. johansenni, S. luggeri, S. piperi, S. vittatum) and compared by restriction fragment length polymorphism (RFLP) analysis. Morphospecies were molecularly distinct, with few shared restriction fragments. Eleven populations of S. vittatum were found that appeared to be homogeneous for a single mitochondrial haplotype. Ten other populations of S. vittatum showed extensive mitochondrial heterogeneity. In part, these samples contained mixtures of two cytologically recognized siblings: IIIL-1 and IS-7. About 70% of the mitochondrial genome of a population pure for sibling IIIL-1 was cloned as five Hind III fragments, which were used as hybridization probes to examine individual black flies. Thirteen mtDNA haplotypes involving permutations of 10 Hind III restriction sites were identified in individual black flies examined from 26 populations. DNA from 168 larvae cut with both EcoRl and Hind III revealed five additional haplotypes. One Hind III haplotype was present in 84% of 390 larvae examined and predominated in every population examined from New York to California and in both the IIIL-1 and IS-7 siblings. Nebraska populations had individuals with nearly all known haplotypes. The most common haplotype was usually the only form present in warm, silty streams with organic enrichment. Rarer haplotypes were found in cool, spring-fed streams but without clear geographic or phylogenetic components.

AB - Mitochondrial DNA (mtDN A) was extracted from pooled field-collected samples representing six species of black flies (Cnephia dacotensis, Simulium bivittaum, S. johansenni, S. luggeri, S. piperi, S. vittatum) and compared by restriction fragment length polymorphism (RFLP) analysis. Morphospecies were molecularly distinct, with few shared restriction fragments. Eleven populations of S. vittatum were found that appeared to be homogeneous for a single mitochondrial haplotype. Ten other populations of S. vittatum showed extensive mitochondrial heterogeneity. In part, these samples contained mixtures of two cytologically recognized siblings: IIIL-1 and IS-7. About 70% of the mitochondrial genome of a population pure for sibling IIIL-1 was cloned as five Hind III fragments, which were used as hybridization probes to examine individual black flies. Thirteen mtDNA haplotypes involving permutations of 10 Hind III restriction sites were identified in individual black flies examined from 26 populations. DNA from 168 larvae cut with both EcoRl and Hind III revealed five additional haplotypes. One Hind III haplotype was present in 84% of 390 larvae examined and predominated in every population examined from New York to California and in both the IIIL-1 and IS-7 siblings. Nebraska populations had individuals with nearly all known haplotypes. The most common haplotype was usually the only form present in warm, silty streams with organic enrichment. Rarer haplotypes were found in cool, spring-fed streams but without clear geographic or phylogenetic components.

UR - http://www.scopus.com/inward/record.url?scp=0031904105&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031904105&partnerID=8YFLogxK

M3 - Article

VL - 76

SP - 440

EP - 447

JO - Canadian Journal of Zoology

JF - Canadian Journal of Zoology

SN - 0008-4301

IS - 3

ER -