miR-27b*, an oxidative stress-responsive microRNA modulates nuclear factor-kB pathway in RAW 264.7 cells

Sivasubramani Thulasingam, Chandirasegaran Massilamany, Arunakumar Gangaplara, Hongjiu Dai, Shahlo Yarbaeva, Sakthivel Subramaniam, Jean-Jack M Riethoven, James D Eudy, Marjorie Lou, N R Jayagopala Reddy

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) produced in macrophages is critical for microbial killing, but they also take part in inflammation and antigen presentation functions. MicroRNAs (miRNAs) are endogenous regulators of gene expression, and they can control immune responses. To dissect the complex nature of ROS-mediated effects in macrophages, we sought to characterize miRNAs that are responsive to oxidative stress-induced with hydrogen peroxide (H 2O2) in the mouse macrophage cell line, RAW 264.7. We have identified a set of unique miRNAs that are differentially expressed in response to H2O2. These include miR-27a*, miR-27b*, miR-29b*, miR-24-2*, and miR-21*, all of which were downregulated except for miR-21*. By using luciferase reporter vector containing nuclear factor-kB (NF-kB) response elements, we demonstrate that overexpression of miR-27b*suppresses lipopolysaccharide-induced activation of NF-kB in RAW 264.7 cells. Our data suggest that macrophage functions can be regulated by oxidative stress-responsive miRNAs by modulating the NF-kB pathway.

Original languageEnglish (US)
Pages (from-to)181-188
Number of pages8
JournalMolecular and cellular biochemistry
Volume352
Issue number1-2
DOIs
StatePublished - Jun 1 2011

Fingerprint

Oxidative stress
Macrophages
MicroRNAs
Oxidative Stress
Reactive Oxygen Species
Antigen Presentation
Response Elements
Regulator Genes
Luciferases
Gene expression
Hydrogen Peroxide
Lipopolysaccharides
Down-Regulation
Chemical activation
Cells
Inflammation
Gene Expression
Antigens
RAW 264.7 Cells

Keywords

  • Hydrogen peroxide
  • Innate immunity
  • Oxidative stress
  • RAW 264.7 cells
  • microRNAs

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

miR-27b*, an oxidative stress-responsive microRNA modulates nuclear factor-kB pathway in RAW 264.7 cells. / Thulasingam, Sivasubramani; Massilamany, Chandirasegaran; Gangaplara, Arunakumar; Dai, Hongjiu; Yarbaeva, Shahlo; Subramaniam, Sakthivel; Riethoven, Jean-Jack M; Eudy, James D; Lou, Marjorie; Reddy, N R Jayagopala.

In: Molecular and cellular biochemistry, Vol. 352, No. 1-2, 01.06.2011, p. 181-188.

Research output: Contribution to journalArticle

Thulasingam, Sivasubramani ; Massilamany, Chandirasegaran ; Gangaplara, Arunakumar ; Dai, Hongjiu ; Yarbaeva, Shahlo ; Subramaniam, Sakthivel ; Riethoven, Jean-Jack M ; Eudy, James D ; Lou, Marjorie ; Reddy, N R Jayagopala. / miR-27b*, an oxidative stress-responsive microRNA modulates nuclear factor-kB pathway in RAW 264.7 cells. In: Molecular and cellular biochemistry. 2011 ; Vol. 352, No. 1-2. pp. 181-188.
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