Minimization of cavity size ensures protein stability and folding: Structures of Phe46-replaced bovine pancreatic RNase A

Tetsuya Kadonosono, Eri Chatani, Rikimaru Hayashi, Hideaki Moriyama, Tatzuo Ueki

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The Phe46 residue, located in the hydrophobic core of RNase A, was replaced with other hydrophobic residues, leucine, valine, or alanine, and their X-ray crystallographic structures were determined up to 1.50-1.80 Å resolution in an attempt to examine the relationship between structural changes and conformational stability or folding kinetics. The backbone structure of F46L, F46V, and F46A was indistinguishable from that of the wild-type enzyme, retaining the correct active site structure. However, one water molecule was included in the hydrophobic core of F46A, forming two hydrogen bonds with the backbone peptide chain. The side chain of Met29 in F46V and F46A adopted two different conformations in an equal occupancy. A trapped water molecule and two conformations of Met29 represent changes that minimize the cavity volume. Nevertheless, the replacement of Phe46 with the above residues resulted in a marked decrease in both thermal stability and folding reaction. Thus, Phe46 ensures the thermal stability and the rapid and correct folding of RNase A by the role it plays in forming a highly packed, hydrophobic core.

Original languageEnglish (US)
Pages (from-to)10651-10658
Number of pages8
JournalBiochemistry
Volume42
Issue number36
DOIs
StatePublished - Sep 16 2003

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Pancreatic Ribonuclease
Protein Stability
Protein Folding
Conformations
Thermodynamic stability
Hot Temperature
Molecules
Water
Valine
Leucine
Alanine
Hydrogen
Catalytic Domain
Hydrogen bonds
Proteins
X-Rays
X rays
Peptides
Kinetics
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Minimization of cavity size ensures protein stability and folding : Structures of Phe46-replaced bovine pancreatic RNase A. / Kadonosono, Tetsuya; Chatani, Eri; Hayashi, Rikimaru; Moriyama, Hideaki; Ueki, Tatzuo.

In: Biochemistry, Vol. 42, No. 36, 16.09.2003, p. 10651-10658.

Research output: Contribution to journalArticle

Kadonosono, Tetsuya ; Chatani, Eri ; Hayashi, Rikimaru ; Moriyama, Hideaki ; Ueki, Tatzuo. / Minimization of cavity size ensures protein stability and folding : Structures of Phe46-replaced bovine pancreatic RNase A. In: Biochemistry. 2003 ; Vol. 42, No. 36. pp. 10651-10658.
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