Minimal information for studies of extracellular vesicles 2018 (MISEV2018)

a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Clotilde Théry, Kenneth W. Witwer, Elena Aikawa, Maria Jose Alcaraz, Johnathon D. Anderson, Ramaroson Andriantsitohaina, Anna Antoniou, Tanina Arab, Fabienne Archer, Georgia K. Atkin-Smith, D. Craig Ayre, Jean Marie Bach, Daniel Bachurski, Hossein Baharvand, Leonora Balaj, Shawn Baldacchino, Natalie N. Bauer, Amy A. Baxter, Mary Bebawy, Carla Beckham & 362 others Apolonija Bedina Zavec, Abderrahim Benmoussa, Anna C. Berardi, Paolo Bergese, Ewa Bielska, Cherie Blenkiron, Sylwia Bobis-Wozowicz, Eric Boilard, Wilfrid Boireau, Antonella Bongiovanni, Francesc E. Borràs, Steffi Bosch, Chantal M. Boulanger, Xandra Breakefield, Andrew M. Breglio, Meadhbh Brennan, David R. Brigstock, Alain Brisson, Marike L.D. Broekman, Jacqueline F. Bromberg, Paulina Bryl-Górecka, Shilpa J Buch, Amy H. Buck, Dylan Burger, Sara Busatto, Dominik Buschmann, Benedetta Bussolati, Edit I. Buzás, James Bryan Byrd, Giovanni Camussi, David R.F. Carter, Sarah Caruso, Lawrence W. Chamley, Yu Ting Chang, Amrita Datta Chaudhuri, Chihchen Chen, Shuai Chen, Lesley Cheng, Andrew R. Chin, Aled Clayton, Stefano P. Clerici, Alex Cocks, Emanuele Cocucci, Robert J. Coffey, Anabela Cordeiro-da-Silva, Yvonne Couch, Frank A.W. Coumans, Beth Coyle, Rossella Crescitelli, Miria Ferreira Criado, Crislyn D’Souza-Schorey, Saumya Das, Paola de Candia, Eliezer F. De Santana, Olivier De Wever, Hernando A. del Portillo, Tanguy Demaret, Sarah Deville, Andrew Devitt, Bert Dhondt, Dolores Di Vizio, Lothar C. Dieterich, Vincenza Dolo, Ana Paula Dominguez Rubio, Massimo Dominici, Mauricio R. Dourado, Tom A.P. Driedonks, Filipe V. Duarte, Heather M. Duncan, Ramon M. Eichenberger, Karin Ekström, Samir EL Andaloussi, Celine Elie-Caille, Uta Erdbrügger, Juan M. Falcón-Pérez, Farah Fatima, Jason E. Fish, Miguel Flores-Bellver, András Försönits, Annie Frelet-Barrand, Fabia Fricke, Gregor Fuhrmann, Susanne Gabrielsson, Ana Gámez-Valero, Chris Gardiner, Kathrin Gärtner, Raphael Gaudin, Yong Song Gho, Bernd Giebel, Caroline Gilbert, Mario Gimona, Ilaria Giusti, Deborah C.I. Goberdhan, André Görgens, Sharon M. Gorski, David W. Greening, Julia Christina Gross, Alice Gualerzi, Gopal N. Gupta, Dakota Gustafson, Aase Handberg, Reka A. Haraszti, Paul Harrison, Hargita Hegyesi, An Hendrix, Andrew F. Hill, Fred H. Hochberg, Karl F. Hoffmann, Beth Holder, Harry Holthofer, Baharak Hosseinkhani, Guoku Hu, Yiyao Huang, Veronica Huber, Stuart Hunt, Ahmed Gamal Eldin Ibrahim, Tsuneya Ikezu, Jameel M. Inal, Mustafa Isin, Alena Ivanova, Hannah K. Jackson, Soren Jacobsen, Steven M. Jay, Muthuvel Jayachandran, Guido Jenster, Lanzhou Jiang, Suzanne M. Johnson, Jennifer C. Jones, Ambrose Jong, Tijana Jovanovic-Talisman, Stephanie Jung, Raghu Kalluri, Shin ichi Kano, Sukhbir Kaur, Yumi Kawamura, Evan T. Keller, Delaram Khamari, Elena Khomyakova, Anastasia Khvorova, Peter Kierulf, Kwang Pyo Kim, Thomas Kislinger, Mikael Klingeborn, David J. Klinke, Miroslaw Kornek, Maja M. Kosanović, Árpád Ferenc Kovács, Eva Maria Krämer-Albers, Susanne Krasemann, Mirja Krause, Igor V. Kurochkin, Gina D. Kusuma, Sören Kuypers, Saara Laitinen, Scott M. Langevin, Lucia R. Languino, Joanne Lannigan, Cecilia Lässer, Louise C. Laurent, Gregory Lavieu, Elisa Lázaro-Ibáñez, Soazig Le Lay, Myung Shin Lee, Yi Xin Fiona Lee, Debora S. Lemos, Metka Lenassi, Aleksandra Leszczynska, Isaac T.S. Li, Ke Liao, Sten F. Libregts, Erzsebet Ligeti, Rebecca Lim, Sai Kiang Lim, Aija Linē, Karen Linnemannstöns, Alicia Llorente, Catherine A. Lombard, Magdalena J. Lorenowicz, Ákos M. Lörincz, Jan Lötvall, Jason Lovett, Michelle C. Lowry, Xavier Loyer, Quan Lu, Barbara Lukomska, Taral R. Lunavat, Sybren L.N. Maas, Harmeet Malhi, Antonio Marcilla, Jacopo Mariani, Javier Mariscal, Elena S. Martens-Uzunova, Lorena Martin-Jaular, M. Carmen Martinez, Vilma Regina Martins, Mathilde Mathieu, Suresh Mathivanan, Marco Maugeri, Lynda K. McGinnis, Mark J. McVey, David G. Meckes, Katie L. Meehan, Inge Mertens, Valentina R. Minciacchi, Andreas Möller, Malene Møller Jørgensen, Aizea Morales-Kastresana, Jess Morhayim, François Mullier, Maurizio Muraca, Luca Musante, Veronika Mussack, Dillon C. Muth, Kathryn H. Myburgh, Tanbir Najrana, Muhammad Nawaz, Irina Nazarenko, Peter Nejsum, Christian Neri, Tommaso Neri, Rienk Nieuwland, Leonardo Nimrichter, John P. Nolan, Esther N.M. Nolte-’t Hoen, Nicole Noren Hooten, Lorraine O’Driscoll, Tina O’Grady, Ana O’Loghlen, Takahiro Ochiya, Martin Olivier, Alberto Ortiz, Luis A. Ortiz, Xabier Osteikoetxea, Ole Ostegaard, Matias Ostrowski, Jaesung Park, D. Michiel Pegtel, Hector Peinado, Francesca Perut, Michael W. Pfaffl, Donald G. Phinney, Bartijn C.H. Pieters, Ryan C. Pink, David S. Pisetsky, Elke Pogge von Strandmann, Iva Polakovicova, Ivan K.H. Poon, Bonita H. Powell, Ilaria Prada, Lynn Pulliam, Peter Quesenberry, Annalisa Radeghieri, Robert L. Raffai, Stefania Raimondo, Janusz Rak, Marcel I. Ramirez, Graça Raposo, Morsi S. Rayyan, Neta Regev-Rudzki, Franz L. Ricklefs, Paul D. Robbins, David D. Roberts, Silvia C. Rodrigues, Eva Rohde, Sophie Rome, Kasper M.A. Rouschop, Aurelia Rughetti, Ashley E. Russell, Paula Saá, Susmita Sahoo, Edison Salas-Huenuleo, Catherine Sánchez, Julie A. Saugstad, Meike J. Saul, Raymond M. Schiffelers, Raphael Schneider, Tine Hiorth Schøyen, Aaron Scott, Eriomina Shahaj, Shivani Sharma, Olga Shatnyeva, Faezeh Shekari, Ganesh Vilas Shelke, Ashok K. Shetty, Kiyotaka Shiba, Pia R.M. Siljander, Andreia M. Silva, Agata Skowronek, Orman L. Snyder, Rodrigo Pedro Soares, Barbara W. Sódar, Carolina Soekmadji, Javier Sotillo, Philip D. Stahl, Willem Stoorvogel, Shannon L. Stott, Erwin F. Strasser, Simon Swift, Hidetoshi Tahara, Muneesh Tewari, Kate Timms, Swasti Tiwari, Rochelle Tixeira, Mercedes Tkach, Wei Seong Toh, Richard Tomasini, Ana Claudia Torrecilhas, Juan Pablo Tosar, Vasilis Toxavidis, Lorena Urbanelli, Pieter Vader, Bas W.M. van Balkom, Susanne G. van der Grein, Jan Van Deun, Martijn J.C. van Herwijnen, Kendall Van Keuren-Jensen, Guillaume van Niel, Martin E. van Royen, Andre J. van Wijnen, M. Helena Vasconcelos, Ivan J. Vechetti, Tiago D. Veit, Laura J. Vella, Émilie Velot, Frederik J. Verweij, Beate Vestad, Jose L. Viñas, Tamás Visnovitz, Krisztina V. Vukman, Jessica Wahlgren, Dionysios C. Watson, Marca H.M. Wauben, Alissa Weaver, Jason P. Webber, Viktoria Weber, Ann M. Wehman, Daniel J. Weiss, Joshua A. Welsh, Sebastian Wendt, Asa M. Wheelock, Zoltán Wiener, Leonie Witte, Joy Wolfram, Angeliki Xagorari, Patricia Xander, Jing Xu, Xiaomei Yan, María Yáñez-Mó, Hang Yin, Yuana Yuana, Valentina Zappulli, Jana Zarubova, Vytautas Žėkas, Jian ye Zhang, Zezhou Zhao, Lei Zheng, Alexander R. Zheutlin, Antje M. Zickler, Pascale Zimmermann, Angela M. Zivkovic, Davide Zocco, Ewa K. Zuba-Surma

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

Original languageEnglish (US)
Article number1535750
JournalJournal of Extracellular Vesicles
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2018

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Guidelines
Exosomes
Cell-Derived Microparticles
Extracellular Vesicles
Checklist
Names
Publications

Keywords

  • ectosomes
  • exosomes
  • extracellular vesicles
  • guidelines
  • microparticles
  • microvesicles
  • minimal information requirements
  • reproducibility
  • rigor
  • standardization

ASJC Scopus subject areas

  • Histology
  • Cell Biology

Cite this

Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. / Théry, Clotilde; Witwer, Kenneth W.; Aikawa, Elena; Alcaraz, Maria Jose; Anderson, Johnathon D.; Andriantsitohaina, Ramaroson; Antoniou, Anna; Arab, Tanina; Archer, Fabienne; Atkin-Smith, Georgia K.; Ayre, D. Craig; Bach, Jean Marie; Bachurski, Daniel; Baharvand, Hossein; Balaj, Leonora; Baldacchino, Shawn; Bauer, Natalie N.; Baxter, Amy A.; Bebawy, Mary; Beckham, Carla; Bedina Zavec, Apolonija; Benmoussa, Abderrahim; Berardi, Anna C.; Bergese, Paolo; Bielska, Ewa; Blenkiron, Cherie; Bobis-Wozowicz, Sylwia; Boilard, Eric; Boireau, Wilfrid; Bongiovanni, Antonella; Borràs, Francesc E.; Bosch, Steffi; Boulanger, Chantal M.; Breakefield, Xandra; Breglio, Andrew M.; Brennan, Meadhbh; Brigstock, David R.; Brisson, Alain; Broekman, Marike L.D.; Bromberg, Jacqueline F.; Bryl-Górecka, Paulina; Buch, Shilpa J; Buck, Amy H.; Burger, Dylan; Busatto, Sara; Buschmann, Dominik; Bussolati, Benedetta; Buzás, Edit I.; Byrd, James Bryan; Camussi, Giovanni; Carter, David R.F.; Caruso, Sarah; Chamley, Lawrence W.; Chang, Yu Ting; Chaudhuri, Amrita Datta; Chen, Chihchen; Chen, Shuai; Cheng, Lesley; Chin, Andrew R.; Clayton, Aled; Clerici, Stefano P.; Cocks, Alex; Cocucci, Emanuele; Coffey, Robert J.; Cordeiro-da-Silva, Anabela; Couch, Yvonne; Coumans, Frank A.W.; Coyle, Beth; Crescitelli, Rossella; Criado, Miria Ferreira; D’Souza-Schorey, Crislyn; Das, Saumya; de Candia, Paola; De Santana, Eliezer F.; De Wever, Olivier; del Portillo, Hernando A.; Demaret, Tanguy; Deville, Sarah; Devitt, Andrew; Dhondt, Bert; Di Vizio, Dolores; Dieterich, Lothar C.; Dolo, Vincenza; Dominguez Rubio, Ana Paula; Dominici, Massimo; Dourado, Mauricio R.; Driedonks, Tom A.P.; Duarte, Filipe V.; Duncan, Heather M.; Eichenberger, Ramon M.; Ekström, Karin; EL Andaloussi, Samir; Elie-Caille, Celine; Erdbrügger, Uta; Falcón-Pérez, Juan M.; Fatima, Farah; Fish, Jason E.; Flores-Bellver, Miguel; Försönits, András; Frelet-Barrand, Annie; Fricke, Fabia; Fuhrmann, Gregor; Gabrielsson, Susanne; Gámez-Valero, Ana; Gardiner, Chris; Gärtner, Kathrin; Gaudin, Raphael; Gho, Yong Song; Giebel, Bernd; Gilbert, Caroline; Gimona, Mario; Giusti, Ilaria; Goberdhan, Deborah C.I.; Görgens, André; Gorski, Sharon M.; Greening, David W.; Gross, Julia Christina; Gualerzi, Alice; Gupta, Gopal N.; Gustafson, Dakota; Handberg, Aase; Haraszti, Reka A.; Harrison, Paul; Hegyesi, Hargita; Hendrix, An; Hill, Andrew F.; Hochberg, Fred H.; Hoffmann, Karl F.; Holder, Beth; Holthofer, Harry; Hosseinkhani, Baharak; Hu, Guoku; Huang, Yiyao; Huber, Veronica; Hunt, Stuart; Ibrahim, Ahmed Gamal Eldin; Ikezu, Tsuneya; Inal, Jameel M.; Isin, Mustafa; Ivanova, Alena; Jackson, Hannah K.; Jacobsen, Soren; Jay, Steven M.; Jayachandran, Muthuvel; Jenster, Guido; Jiang, Lanzhou; Johnson, Suzanne M.; Jones, Jennifer C.; Jong, Ambrose; Jovanovic-Talisman, Tijana; Jung, Stephanie; Kalluri, Raghu; Kano, Shin ichi; Kaur, Sukhbir; Kawamura, Yumi; Keller, Evan T.; Khamari, Delaram; Khomyakova, Elena; Khvorova, Anastasia; Kierulf, Peter; Kim, Kwang Pyo; Kislinger, Thomas; Klingeborn, Mikael; Klinke, David J.; Kornek, Miroslaw; Kosanović, Maja M.; Kovács, Árpád Ferenc; Krämer-Albers, Eva Maria; Krasemann, Susanne; Krause, Mirja; Kurochkin, Igor V.; Kusuma, Gina D.; Kuypers, Sören; Laitinen, Saara; Langevin, Scott M.; Languino, Lucia R.; Lannigan, Joanne; Lässer, Cecilia; Laurent, Louise C.; Lavieu, Gregory; Lázaro-Ibáñez, Elisa; Le Lay, Soazig; Lee, Myung Shin; Lee, Yi Xin Fiona; Lemos, Debora S.; Lenassi, Metka; Leszczynska, Aleksandra; Li, Isaac T.S.; Liao, Ke; Libregts, Sten F.; Ligeti, Erzsebet; Lim, Rebecca; Lim, Sai Kiang; Linē, Aija; Linnemannstöns, Karen; Llorente, Alicia; Lombard, Catherine A.; Lorenowicz, Magdalena J.; Lörincz, Ákos M.; Lötvall, Jan; Lovett, Jason; Lowry, Michelle C.; Loyer, Xavier; Lu, Quan; Lukomska, Barbara; Lunavat, Taral R.; Maas, Sybren L.N.; Malhi, Harmeet; Marcilla, Antonio; Mariani, Jacopo; Mariscal, Javier; Martens-Uzunova, Elena S.; Martin-Jaular, Lorena; Martinez, M. Carmen; Martins, Vilma Regina; Mathieu, Mathilde; Mathivanan, Suresh; Maugeri, Marco; McGinnis, Lynda K.; McVey, Mark J.; Meckes, David G.; Meehan, Katie L.; Mertens, Inge; Minciacchi, Valentina R.; Möller, Andreas; Møller Jørgensen, Malene; Morales-Kastresana, Aizea; Morhayim, Jess; Mullier, François; Muraca, Maurizio; Musante, Luca; Mussack, Veronika; Muth, Dillon C.; Myburgh, Kathryn H.; Najrana, Tanbir; Nawaz, Muhammad; Nazarenko, Irina; Nejsum, Peter; Neri, Christian; Neri, Tommaso; Nieuwland, Rienk; Nimrichter, Leonardo; Nolan, John P.; Nolte-’t Hoen, Esther N.M.; Noren Hooten, Nicole; O’Driscoll, Lorraine; O’Grady, Tina; O’Loghlen, Ana; Ochiya, Takahiro; Olivier, Martin; Ortiz, Alberto; Ortiz, Luis A.; Osteikoetxea, Xabier; Ostegaard, Ole; Ostrowski, Matias; Park, Jaesung; Pegtel, D. Michiel; Peinado, Hector; Perut, Francesca; Pfaffl, Michael W.; Phinney, Donald G.; Pieters, Bartijn C.H.; Pink, Ryan C.; Pisetsky, David S.; Pogge von Strandmann, Elke; Polakovicova, Iva; Poon, Ivan K.H.; Powell, Bonita H.; Prada, Ilaria; Pulliam, Lynn; Quesenberry, Peter; Radeghieri, Annalisa; Raffai, Robert L.; Raimondo, Stefania; Rak, Janusz; Ramirez, Marcel I.; Raposo, Graça; Rayyan, Morsi S.; Regev-Rudzki, Neta; Ricklefs, Franz L.; Robbins, Paul D.; Roberts, David D.; Rodrigues, Silvia C.; Rohde, Eva; Rome, Sophie; Rouschop, Kasper M.A.; Rughetti, Aurelia; Russell, Ashley E.; Saá, Paula; Sahoo, Susmita; Salas-Huenuleo, Edison; Sánchez, Catherine; Saugstad, Julie A.; Saul, Meike J.; Schiffelers, Raymond M.; Schneider, Raphael; Schøyen, Tine Hiorth; Scott, Aaron; Shahaj, Eriomina; Sharma, Shivani; Shatnyeva, Olga; Shekari, Faezeh; Shelke, Ganesh Vilas; Shetty, Ashok K.; Shiba, Kiyotaka; Siljander, Pia R.M.; Silva, Andreia M.; Skowronek, Agata; Snyder, Orman L.; Soares, Rodrigo Pedro; Sódar, Barbara W.; Soekmadji, Carolina; Sotillo, Javier; Stahl, Philip D.; Stoorvogel, Willem; Stott, Shannon L.; Strasser, Erwin F.; Swift, Simon; Tahara, Hidetoshi; Tewari, Muneesh; Timms, Kate; Tiwari, Swasti; Tixeira, Rochelle; Tkach, Mercedes; Toh, Wei Seong; Tomasini, Richard; Torrecilhas, Ana Claudia; Tosar, Juan Pablo; Toxavidis, Vasilis; Urbanelli, Lorena; Vader, Pieter; van Balkom, Bas W.M.; van der Grein, Susanne G.; Van Deun, Jan; van Herwijnen, Martijn J.C.; Van Keuren-Jensen, Kendall; van Niel, Guillaume; van Royen, Martin E.; van Wijnen, Andre J.; Vasconcelos, M. Helena; Vechetti, Ivan J.; Veit, Tiago D.; Vella, Laura J.; Velot, Émilie; Verweij, Frederik J.; Vestad, Beate; Viñas, Jose L.; Visnovitz, Tamás; Vukman, Krisztina V.; Wahlgren, Jessica; Watson, Dionysios C.; Wauben, Marca H.M.; Weaver, Alissa; Webber, Jason P.; Weber, Viktoria; Wehman, Ann M.; Weiss, Daniel J.; Welsh, Joshua A.; Wendt, Sebastian; Wheelock, Asa M.; Wiener, Zoltán; Witte, Leonie; Wolfram, Joy; Xagorari, Angeliki; Xander, Patricia; Xu, Jing; Yan, Xiaomei; Yáñez-Mó, María; Yin, Hang; Yuana, Yuana; Zappulli, Valentina; Zarubova, Jana; Žėkas, Vytautas; Zhang, Jian ye; Zhao, Zezhou; Zheng, Lei; Zheutlin, Alexander R.; Zickler, Antje M.; Zimmermann, Pascale; Zivkovic, Angela M.; Zocco, Davide; Zuba-Surma, Ewa K.

In: Journal of Extracellular Vesicles, Vol. 7, No. 1, 1535750, 01.01.2018.

Research output: Contribution to journalArticle

Théry, C, Witwer, KW, Aikawa, E, Alcaraz, MJ, Anderson, JD, Andriantsitohaina, R, Antoniou, A, Arab, T, Archer, F, Atkin-Smith, GK, Ayre, DC, Bach, JM, Bachurski, D, Baharvand, H, Balaj, L, Baldacchino, S, Bauer, NN, Baxter, AA, Bebawy, M, Beckham, C, Bedina Zavec, A, Benmoussa, A, Berardi, AC, Bergese, P, Bielska, E, Blenkiron, C, Bobis-Wozowicz, S, Boilard, E, Boireau, W, Bongiovanni, A, Borràs, FE, Bosch, S, Boulanger, CM, Breakefield, X, Breglio, AM, Brennan, M, Brigstock, DR, Brisson, A, Broekman, MLD, Bromberg, JF, Bryl-Górecka, P, Buch, SJ, Buck, AH, Burger, D, Busatto, S, Buschmann, D, Bussolati, B, Buzás, EI, Byrd, JB, Camussi, G, Carter, DRF, Caruso, S, Chamley, LW, Chang, YT, Chaudhuri, AD, Chen, C, Chen, S, Cheng, L, Chin, AR, Clayton, A, Clerici, SP, Cocks, A, Cocucci, E, Coffey, RJ, Cordeiro-da-Silva, A, Couch, Y, Coumans, FAW, Coyle, B, Crescitelli, R, Criado, MF, D’Souza-Schorey, C, Das, S, de Candia, P, De Santana, EF, De Wever, O, del Portillo, HA, Demaret, T, Deville, S, Devitt, A, Dhondt, B, Di Vizio, D, Dieterich, LC, Dolo, V, Dominguez Rubio, AP, Dominici, M, Dourado, MR, Driedonks, TAP, Duarte, FV, Duncan, HM, Eichenberger, RM, Ekström, K, EL Andaloussi, S, Elie-Caille, C, Erdbrügger, U, Falcón-Pérez, JM, Fatima, F, Fish, JE, Flores-Bellver, M, Försönits, A, Frelet-Barrand, A, Fricke, F, Fuhrmann, G, Gabrielsson, S, Gámez-Valero, A, Gardiner, C, Gärtner, K, Gaudin, R, Gho, YS, Giebel, B, Gilbert, C, Gimona, M, Giusti, I, Goberdhan, DCI, Görgens, A, Gorski, SM, Greening, DW, Gross, JC, Gualerzi, A, Gupta, GN, Gustafson, D, Handberg, A, Haraszti, RA, Harrison, P, Hegyesi, H, Hendrix, A, Hill, AF, Hochberg, FH, Hoffmann, KF, Holder, B, Holthofer, H, Hosseinkhani, B, Hu, G, Huang, Y, Huber, V, Hunt, S, Ibrahim, AGE, Ikezu, T, Inal, JM, Isin, M, Ivanova, A, Jackson, HK, Jacobsen, S, Jay, SM, Jayachandran, M, Jenster, G, Jiang, L, Johnson, SM, Jones, JC, Jong, A, Jovanovic-Talisman, T, Jung, S, Kalluri, R, Kano, SI, Kaur, S, Kawamura, Y, Keller, ET, Khamari, D, Khomyakova, E, Khvorova, A, Kierulf, P, Kim, KP, Kislinger, T, Klingeborn, M, Klinke, DJ, Kornek, M, Kosanović, MM, Kovács, ÁF, Krämer-Albers, EM, Krasemann, S, Krause, M, Kurochkin, IV, Kusuma, GD, Kuypers, S, Laitinen, S, Langevin, SM, Languino, LR, Lannigan, J, Lässer, C, Laurent, LC, Lavieu, G, Lázaro-Ibáñez, E, Le Lay, S, Lee, MS, Lee, YXF, Lemos, DS, Lenassi, M, Leszczynska, A, Li, ITS, Liao, K, Libregts, SF, Ligeti, E, Lim, R, Lim, SK, Linē, A, Linnemannstöns, K, Llorente, A, Lombard, CA, Lorenowicz, MJ, Lörincz, ÁM, Lötvall, J, Lovett, J, Lowry, MC, Loyer, X, Lu, Q, Lukomska, B, Lunavat, TR, Maas, SLN, Malhi, H, Marcilla, A, Mariani, J, Mariscal, J, Martens-Uzunova, ES, Martin-Jaular, L, Martinez, MC, Martins, VR, Mathieu, M, Mathivanan, S, Maugeri, M, McGinnis, LK, McVey, MJ, Meckes, DG, Meehan, KL, Mertens, I, Minciacchi, VR, Möller, A, Møller Jørgensen, M, Morales-Kastresana, A, Morhayim, J, Mullier, F, Muraca, M, Musante, L, Mussack, V, Muth, DC, Myburgh, KH, Najrana, T, Nawaz, M, Nazarenko, I, Nejsum, P, Neri, C, Neri, T, Nieuwland, R, Nimrichter, L, Nolan, JP, Nolte-’t Hoen, ENM, Noren Hooten, N, O’Driscoll, L, O’Grady, T, O’Loghlen, A, Ochiya, T, Olivier, M, Ortiz, A, Ortiz, LA, Osteikoetxea, X, Ostegaard, O, Ostrowski, M, Park, J, Pegtel, DM, Peinado, H, Perut, F, Pfaffl, MW, Phinney, DG, Pieters, BCH, Pink, RC, Pisetsky, DS, Pogge von Strandmann, E, Polakovicova, I, Poon, IKH, Powell, BH, Prada, I, Pulliam, L, Quesenberry, P, Radeghieri, A, Raffai, RL, Raimondo, S, Rak, J, Ramirez, MI, Raposo, G, Rayyan, MS, Regev-Rudzki, N, Ricklefs, FL, Robbins, PD, Roberts, DD, Rodrigues, SC, Rohde, E, Rome, S, Rouschop, KMA, Rughetti, A, Russell, AE, Saá, P, Sahoo, S, Salas-Huenuleo, E, Sánchez, C, Saugstad, JA, Saul, MJ, Schiffelers, RM, Schneider, R, Schøyen, TH, Scott, A, Shahaj, E, Sharma, S, Shatnyeva, O, Shekari, F, Shelke, GV, Shetty, AK, Shiba, K, Siljander, PRM, Silva, AM, Skowronek, A, Snyder, OL, Soares, RP, Sódar, BW, Soekmadji, C, Sotillo, J, Stahl, PD, Stoorvogel, W, Stott, SL, Strasser, EF, Swift, S, Tahara, H, Tewari, M, Timms, K, Tiwari, S, Tixeira, R, Tkach, M, Toh, WS, Tomasini, R, Torrecilhas, AC, Tosar, JP, Toxavidis, V, Urbanelli, L, Vader, P, van Balkom, BWM, van der Grein, SG, Van Deun, J, van Herwijnen, MJC, Van Keuren-Jensen, K, van Niel, G, van Royen, ME, van Wijnen, AJ, Vasconcelos, MH, Vechetti, IJ, Veit, TD, Vella, LJ, Velot, É, Verweij, FJ, Vestad, B, Viñas, JL, Visnovitz, T, Vukman, KV, Wahlgren, J, Watson, DC, Wauben, MHM, Weaver, A, Webber, JP, Weber, V, Wehman, AM, Weiss, DJ, Welsh, JA, Wendt, S, Wheelock, AM, Wiener, Z, Witte, L, Wolfram, J, Xagorari, A, Xander, P, Xu, J, Yan, X, Yáñez-Mó, M, Yin, H, Yuana, Y, Zappulli, V, Zarubova, J, Žėkas, V, Zhang, JY, Zhao, Z, Zheng, L, Zheutlin, AR, Zickler, AM, Zimmermann, P, Zivkovic, AM, Zocco, D & Zuba-Surma, EK 2018, 'Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines', Journal of Extracellular Vesicles, vol. 7, no. 1, 1535750. https://doi.org/10.1080/20013078.2018.1535750
Théry, Clotilde ; Witwer, Kenneth W. ; Aikawa, Elena ; Alcaraz, Maria Jose ; Anderson, Johnathon D. ; Andriantsitohaina, Ramaroson ; Antoniou, Anna ; Arab, Tanina ; Archer, Fabienne ; Atkin-Smith, Georgia K. ; Ayre, D. Craig ; Bach, Jean Marie ; Bachurski, Daniel ; Baharvand, Hossein ; Balaj, Leonora ; Baldacchino, Shawn ; Bauer, Natalie N. ; Baxter, Amy A. ; Bebawy, Mary ; Beckham, Carla ; Bedina Zavec, Apolonija ; Benmoussa, Abderrahim ; Berardi, Anna C. ; Bergese, Paolo ; Bielska, Ewa ; Blenkiron, Cherie ; Bobis-Wozowicz, Sylwia ; Boilard, Eric ; Boireau, Wilfrid ; Bongiovanni, Antonella ; Borràs, Francesc E. ; Bosch, Steffi ; Boulanger, Chantal M. ; Breakefield, Xandra ; Breglio, Andrew M. ; Brennan, Meadhbh ; Brigstock, David R. ; Brisson, Alain ; Broekman, Marike L.D. ; Bromberg, Jacqueline F. ; Bryl-Górecka, Paulina ; Buch, Shilpa J ; Buck, Amy H. ; Burger, Dylan ; Busatto, Sara ; Buschmann, Dominik ; Bussolati, Benedetta ; Buzás, Edit I. ; Byrd, James Bryan ; Camussi, Giovanni ; Carter, David R.F. ; Caruso, Sarah ; Chamley, Lawrence W. ; Chang, Yu Ting ; Chaudhuri, Amrita Datta ; Chen, Chihchen ; Chen, Shuai ; Cheng, Lesley ; Chin, Andrew R. ; Clayton, Aled ; Clerici, Stefano P. ; Cocks, Alex ; Cocucci, Emanuele ; Coffey, Robert J. ; Cordeiro-da-Silva, Anabela ; Couch, Yvonne ; Coumans, Frank A.W. ; Coyle, Beth ; Crescitelli, Rossella ; Criado, Miria Ferreira ; D’Souza-Schorey, Crislyn ; Das, Saumya ; de Candia, Paola ; De Santana, Eliezer F. ; De Wever, Olivier ; del Portillo, Hernando A. ; Demaret, Tanguy ; Deville, Sarah ; Devitt, Andrew ; Dhondt, Bert ; Di Vizio, Dolores ; Dieterich, Lothar C. ; Dolo, Vincenza ; Dominguez Rubio, Ana Paula ; Dominici, Massimo ; Dourado, Mauricio R. ; Driedonks, Tom A.P. ; Duarte, Filipe V. ; Duncan, Heather M. ; Eichenberger, Ramon M. ; Ekström, Karin ; EL Andaloussi, Samir ; Elie-Caille, Celine ; Erdbrügger, Uta ; Falcón-Pérez, Juan M. ; Fatima, Farah ; Fish, Jason E. ; Flores-Bellver, Miguel ; Försönits, András ; Frelet-Barrand, Annie ; Fricke, Fabia ; Fuhrmann, Gregor ; Gabrielsson, Susanne ; Gámez-Valero, Ana ; Gardiner, Chris ; Gärtner, Kathrin ; Gaudin, Raphael ; Gho, Yong Song ; Giebel, Bernd ; Gilbert, Caroline ; Gimona, Mario ; Giusti, Ilaria ; Goberdhan, Deborah C.I. ; Görgens, André ; Gorski, Sharon M. ; Greening, David W. ; Gross, Julia Christina ; Gualerzi, Alice ; Gupta, Gopal N. ; Gustafson, Dakota ; Handberg, Aase ; Haraszti, Reka A. ; Harrison, Paul ; Hegyesi, Hargita ; Hendrix, An ; Hill, Andrew F. ; Hochberg, Fred H. ; Hoffmann, Karl F. ; Holder, Beth ; Holthofer, Harry ; Hosseinkhani, Baharak ; Hu, Guoku ; Huang, Yiyao ; Huber, Veronica ; Hunt, Stuart ; Ibrahim, Ahmed Gamal Eldin ; Ikezu, Tsuneya ; Inal, Jameel M. ; Isin, Mustafa ; Ivanova, Alena ; Jackson, Hannah K. ; Jacobsen, Soren ; Jay, Steven M. ; Jayachandran, Muthuvel ; Jenster, Guido ; Jiang, Lanzhou ; Johnson, Suzanne M. ; Jones, Jennifer C. ; Jong, Ambrose ; Jovanovic-Talisman, Tijana ; Jung, Stephanie ; Kalluri, Raghu ; Kano, Shin ichi ; Kaur, Sukhbir ; Kawamura, Yumi ; Keller, Evan T. ; Khamari, Delaram ; Khomyakova, Elena ; Khvorova, Anastasia ; Kierulf, Peter ; Kim, Kwang Pyo ; Kislinger, Thomas ; Klingeborn, Mikael ; Klinke, David J. ; Kornek, Miroslaw ; Kosanović, Maja M. ; Kovács, Árpád Ferenc ; Krämer-Albers, Eva Maria ; Krasemann, Susanne ; Krause, Mirja ; Kurochkin, Igor V. ; Kusuma, Gina D. ; Kuypers, Sören ; Laitinen, Saara ; Langevin, Scott M. ; Languino, Lucia R. ; Lannigan, Joanne ; Lässer, Cecilia ; Laurent, Louise C. ; Lavieu, Gregory ; Lázaro-Ibáñez, Elisa ; Le Lay, Soazig ; Lee, Myung Shin ; Lee, Yi Xin Fiona ; Lemos, Debora S. ; Lenassi, Metka ; Leszczynska, Aleksandra ; Li, Isaac T.S. ; Liao, Ke ; Libregts, Sten F. ; Ligeti, Erzsebet ; Lim, Rebecca ; Lim, Sai Kiang ; Linē, Aija ; Linnemannstöns, Karen ; Llorente, Alicia ; Lombard, Catherine A. ; Lorenowicz, Magdalena J. ; Lörincz, Ákos M. ; Lötvall, Jan ; Lovett, Jason ; Lowry, Michelle C. ; Loyer, Xavier ; Lu, Quan ; Lukomska, Barbara ; Lunavat, Taral R. ; Maas, Sybren L.N. ; Malhi, Harmeet ; Marcilla, Antonio ; Mariani, Jacopo ; Mariscal, Javier ; Martens-Uzunova, Elena S. ; Martin-Jaular, Lorena ; Martinez, M. Carmen ; Martins, Vilma Regina ; Mathieu, Mathilde ; Mathivanan, Suresh ; Maugeri, Marco ; McGinnis, Lynda K. ; McVey, Mark J. ; Meckes, David G. ; Meehan, Katie L. ; Mertens, Inge ; Minciacchi, Valentina R. ; Möller, Andreas ; Møller Jørgensen, Malene ; Morales-Kastresana, Aizea ; Morhayim, Jess ; Mullier, François ; Muraca, Maurizio ; Musante, Luca ; Mussack, Veronika ; Muth, Dillon C. ; Myburgh, Kathryn H. ; Najrana, Tanbir ; Nawaz, Muhammad ; Nazarenko, Irina ; Nejsum, Peter ; Neri, Christian ; Neri, Tommaso ; Nieuwland, Rienk ; Nimrichter, Leonardo ; Nolan, John P. ; Nolte-’t Hoen, Esther N.M. ; Noren Hooten, Nicole ; O’Driscoll, Lorraine ; O’Grady, Tina ; O’Loghlen, Ana ; Ochiya, Takahiro ; Olivier, Martin ; Ortiz, Alberto ; Ortiz, Luis A. ; Osteikoetxea, Xabier ; Ostegaard, Ole ; Ostrowski, Matias ; Park, Jaesung ; Pegtel, D. Michiel ; Peinado, Hector ; Perut, Francesca ; Pfaffl, Michael W. ; Phinney, Donald G. ; Pieters, Bartijn C.H. ; Pink, Ryan C. ; Pisetsky, David S. ; Pogge von Strandmann, Elke ; Polakovicova, Iva ; Poon, Ivan K.H. ; Powell, Bonita H. ; Prada, Ilaria ; Pulliam, Lynn ; Quesenberry, Peter ; Radeghieri, Annalisa ; Raffai, Robert L. ; Raimondo, Stefania ; Rak, Janusz ; Ramirez, Marcel I. ; Raposo, Graça ; Rayyan, Morsi S. ; Regev-Rudzki, Neta ; Ricklefs, Franz L. ; Robbins, Paul D. ; Roberts, David D. ; Rodrigues, Silvia C. ; Rohde, Eva ; Rome, Sophie ; Rouschop, Kasper M.A. ; Rughetti, Aurelia ; Russell, Ashley E. ; Saá, Paula ; Sahoo, Susmita ; Salas-Huenuleo, Edison ; Sánchez, Catherine ; Saugstad, Julie A. ; Saul, Meike J. ; Schiffelers, Raymond M. ; Schneider, Raphael ; Schøyen, Tine Hiorth ; Scott, Aaron ; Shahaj, Eriomina ; Sharma, Shivani ; Shatnyeva, Olga ; Shekari, Faezeh ; Shelke, Ganesh Vilas ; Shetty, Ashok K. ; Shiba, Kiyotaka ; Siljander, Pia R.M. ; Silva, Andreia M. ; Skowronek, Agata ; Snyder, Orman L. ; Soares, Rodrigo Pedro ; Sódar, Barbara W. ; Soekmadji, Carolina ; Sotillo, Javier ; Stahl, Philip D. ; Stoorvogel, Willem ; Stott, Shannon L. ; Strasser, Erwin F. ; Swift, Simon ; Tahara, Hidetoshi ; Tewari, Muneesh ; Timms, Kate ; Tiwari, Swasti ; Tixeira, Rochelle ; Tkach, Mercedes ; Toh, Wei Seong ; Tomasini, Richard ; Torrecilhas, Ana Claudia ; Tosar, Juan Pablo ; Toxavidis, Vasilis ; Urbanelli, Lorena ; Vader, Pieter ; van Balkom, Bas W.M. ; van der Grein, Susanne G. ; Van Deun, Jan ; van Herwijnen, Martijn J.C. ; Van Keuren-Jensen, Kendall ; van Niel, Guillaume ; van Royen, Martin E. ; van Wijnen, Andre J. ; Vasconcelos, M. Helena ; Vechetti, Ivan J. ; Veit, Tiago D. ; Vella, Laura J. ; Velot, Émilie ; Verweij, Frederik J. ; Vestad, Beate ; Viñas, Jose L. ; Visnovitz, Tamás ; Vukman, Krisztina V. ; Wahlgren, Jessica ; Watson, Dionysios C. ; Wauben, Marca H.M. ; Weaver, Alissa ; Webber, Jason P. ; Weber, Viktoria ; Wehman, Ann M. ; Weiss, Daniel J. ; Welsh, Joshua A. ; Wendt, Sebastian ; Wheelock, Asa M. ; Wiener, Zoltán ; Witte, Leonie ; Wolfram, Joy ; Xagorari, Angeliki ; Xander, Patricia ; Xu, Jing ; Yan, Xiaomei ; Yáñez-Mó, María ; Yin, Hang ; Yuana, Yuana ; Zappulli, Valentina ; Zarubova, Jana ; Žėkas, Vytautas ; Zhang, Jian ye ; Zhao, Zezhou ; Zheng, Lei ; Zheutlin, Alexander R. ; Zickler, Antje M. ; Zimmermann, Pascale ; Zivkovic, Angela M. ; Zocco, Davide ; Zuba-Surma, Ewa K. / Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. In: Journal of Extracellular Vesicles. 2018 ; Vol. 7, No. 1.
@article{3247f5b550ae4bdf80141d445362a913,
title = "Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines",
abstract = "The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.",
keywords = "ectosomes, exosomes, extracellular vesicles, guidelines, microparticles, microvesicles, minimal information requirements, reproducibility, rigor, standardization",
author = "Clotilde Th{\'e}ry and Witwer, {Kenneth W.} and Elena Aikawa and Alcaraz, {Maria Jose} and Anderson, {Johnathon D.} and Ramaroson Andriantsitohaina and Anna Antoniou and Tanina Arab and Fabienne Archer and Atkin-Smith, {Georgia K.} and Ayre, {D. Craig} and Bach, {Jean Marie} and Daniel Bachurski and Hossein Baharvand and Leonora Balaj and Shawn Baldacchino and Bauer, {Natalie N.} and Baxter, {Amy A.} and Mary Bebawy and Carla Beckham and {Bedina Zavec}, Apolonija and Abderrahim Benmoussa and Berardi, {Anna C.} and Paolo Bergese and Ewa Bielska and Cherie Blenkiron and Sylwia Bobis-Wozowicz and Eric Boilard and Wilfrid Boireau and Antonella Bongiovanni and Borr{\`a}s, {Francesc E.} and Steffi Bosch and Boulanger, {Chantal M.} and Xandra Breakefield and Breglio, {Andrew M.} and Meadhbh Brennan and Brigstock, {David R.} and Alain Brisson and Broekman, {Marike L.D.} and Bromberg, {Jacqueline F.} and Paulina Bryl-G{\'o}recka and Buch, {Shilpa J} and Buck, {Amy H.} and Dylan Burger and Sara Busatto and Dominik Buschmann and Benedetta Bussolati and Buz{\'a}s, {Edit I.} and Byrd, {James Bryan} and Giovanni Camussi and Carter, {David R.F.} and Sarah Caruso and Chamley, {Lawrence W.} and Chang, {Yu Ting} and Chaudhuri, {Amrita Datta} and Chihchen Chen and Shuai Chen and Lesley Cheng and Chin, {Andrew R.} and Aled Clayton and Clerici, {Stefano P.} and Alex Cocks and Emanuele Cocucci and Coffey, {Robert J.} and Anabela Cordeiro-da-Silva and Yvonne Couch and Coumans, {Frank A.W.} and Beth Coyle and Rossella Crescitelli and Criado, {Miria Ferreira} and Crislyn D’Souza-Schorey and Saumya Das and {de Candia}, Paola and {De Santana}, {Eliezer F.} and {De Wever}, Olivier and {del Portillo}, {Hernando A.} and Tanguy Demaret and Sarah Deville and Andrew Devitt and Bert Dhondt and {Di Vizio}, Dolores and Dieterich, {Lothar C.} and Vincenza Dolo and {Dominguez Rubio}, {Ana Paula} and Massimo Dominici and Dourado, {Mauricio R.} and Driedonks, {Tom A.P.} and Duarte, {Filipe V.} and Duncan, {Heather M.} and Eichenberger, {Ramon M.} and Karin Ekstr{\"o}m and {EL Andaloussi}, Samir and Celine Elie-Caille and Uta Erdbr{\"u}gger and Falc{\'o}n-P{\'e}rez, {Juan M.} and Farah Fatima and Fish, {Jason E.} and Miguel Flores-Bellver and Andr{\'a}s F{\"o}rs{\"o}nits and Annie Frelet-Barrand and Fabia Fricke and Gregor Fuhrmann and Susanne Gabrielsson and Ana G{\'a}mez-Valero and Chris Gardiner and Kathrin G{\"a}rtner and Raphael Gaudin and Gho, {Yong Song} and Bernd Giebel and Caroline Gilbert and Mario Gimona and Ilaria Giusti and Goberdhan, {Deborah C.I.} and Andr{\'e} G{\"o}rgens and Gorski, {Sharon M.} and Greening, {David W.} and Gross, {Julia Christina} and Alice Gualerzi and Gupta, {Gopal N.} and Dakota Gustafson and Aase Handberg and Haraszti, {Reka A.} and Paul Harrison and Hargita Hegyesi and An Hendrix and Hill, {Andrew F.} and Hochberg, {Fred H.} and Hoffmann, {Karl F.} and Beth Holder and Harry Holthofer and Baharak Hosseinkhani and Guoku Hu and Yiyao Huang and Veronica Huber and Stuart Hunt and Ibrahim, {Ahmed Gamal Eldin} and Tsuneya Ikezu and Inal, {Jameel M.} and Mustafa Isin and Alena Ivanova and Jackson, {Hannah K.} and Soren Jacobsen and Jay, {Steven M.} and Muthuvel Jayachandran and Guido Jenster and Lanzhou Jiang and Johnson, {Suzanne M.} and Jones, {Jennifer C.} and Ambrose Jong and Tijana Jovanovic-Talisman and Stephanie Jung and Raghu Kalluri and Kano, {Shin ichi} and Sukhbir Kaur and Yumi Kawamura and Keller, {Evan T.} and Delaram Khamari and Elena Khomyakova and Anastasia Khvorova and Peter Kierulf and Kim, {Kwang Pyo} and Thomas Kislinger and Mikael Klingeborn and Klinke, {David J.} and Miroslaw Kornek and Kosanović, {Maja M.} and Kov{\'a}cs, {{\'A}rp{\'a}d Ferenc} and Kr{\"a}mer-Albers, {Eva Maria} and Susanne Krasemann and Mirja Krause and Kurochkin, {Igor V.} and Kusuma, {Gina D.} and S{\"o}ren Kuypers and Saara Laitinen and Langevin, {Scott M.} and Languino, {Lucia R.} and Joanne Lannigan and Cecilia L{\"a}sser and Laurent, {Louise C.} and Gregory Lavieu and Elisa L{\'a}zaro-Ib{\'a}{\~n}ez and {Le Lay}, Soazig and Lee, {Myung Shin} and Lee, {Yi Xin Fiona} and Lemos, {Debora S.} and Metka Lenassi and Aleksandra Leszczynska and Li, {Isaac T.S.} and Ke Liao and Libregts, {Sten F.} and Erzsebet Ligeti and Rebecca Lim and Lim, {Sai Kiang} and Aija Linē and Karen Linnemannst{\"o}ns and Alicia Llorente and Lombard, {Catherine A.} and Lorenowicz, {Magdalena J.} and L{\"o}rincz, {{\'A}kos M.} and Jan L{\"o}tvall and Jason Lovett and Lowry, {Michelle C.} and Xavier Loyer and Quan Lu and Barbara Lukomska and Lunavat, {Taral R.} and Maas, {Sybren L.N.} and Harmeet Malhi and Antonio Marcilla and Jacopo Mariani and Javier Mariscal and Martens-Uzunova, {Elena S.} and Lorena Martin-Jaular and Martinez, {M. Carmen} and Martins, {Vilma Regina} and Mathilde Mathieu and Suresh Mathivanan and Marco Maugeri and McGinnis, {Lynda K.} and McVey, {Mark J.} and Meckes, {David G.} and Meehan, {Katie L.} and Inge Mertens and Minciacchi, {Valentina R.} and Andreas M{\"o}ller and {M{\o}ller J{\o}rgensen}, Malene and Aizea Morales-Kastresana and Jess Morhayim and Fran{\cc}ois Mullier and Maurizio Muraca and Luca Musante and Veronika Mussack and Muth, {Dillon C.} and Myburgh, {Kathryn H.} and Tanbir Najrana and Muhammad Nawaz and Irina Nazarenko and Peter Nejsum and Christian Neri and Tommaso Neri and Rienk Nieuwland and Leonardo Nimrichter and Nolan, {John P.} and {Nolte-’t Hoen}, {Esther N.M.} and {Noren Hooten}, Nicole and Lorraine O’Driscoll and Tina O’Grady and Ana O’Loghlen and Takahiro Ochiya and Martin Olivier and Alberto Ortiz and Ortiz, {Luis A.} and Xabier Osteikoetxea and Ole Ostegaard and Matias Ostrowski and Jaesung Park and Pegtel, {D. Michiel} and Hector Peinado and Francesca Perut and Pfaffl, {Michael W.} and Phinney, {Donald G.} and Pieters, {Bartijn C.H.} and Pink, {Ryan C.} and Pisetsky, {David S.} and {Pogge von Strandmann}, Elke and Iva Polakovicova and Poon, {Ivan K.H.} and Powell, {Bonita H.} and Ilaria Prada and Lynn Pulliam and Peter Quesenberry and Annalisa Radeghieri and Raffai, {Robert L.} and Stefania Raimondo and Janusz Rak and Ramirez, {Marcel I.} and Gra{\cc}a Raposo and Rayyan, {Morsi S.} and Neta Regev-Rudzki and Ricklefs, {Franz L.} and Robbins, {Paul D.} and Roberts, {David D.} and Rodrigues, {Silvia C.} and Eva Rohde and Sophie Rome and Rouschop, {Kasper M.A.} and Aurelia Rughetti and Russell, {Ashley E.} and Paula Sa{\'a} and Susmita Sahoo and Edison Salas-Huenuleo and Catherine S{\'a}nchez and Saugstad, {Julie A.} and Saul, {Meike J.} and Schiffelers, {Raymond M.} and Raphael Schneider and Sch{\o}yen, {Tine Hiorth} and Aaron Scott and Eriomina Shahaj and Shivani Sharma and Olga Shatnyeva and Faezeh Shekari and Shelke, {Ganesh Vilas} and Shetty, {Ashok K.} and Kiyotaka Shiba and Siljander, {Pia R.M.} and Silva, {Andreia M.} and Agata Skowronek and Snyder, {Orman L.} and Soares, {Rodrigo Pedro} and S{\'o}dar, {Barbara W.} and Carolina Soekmadji and Javier Sotillo and Stahl, {Philip D.} and Willem Stoorvogel and Stott, {Shannon L.} and Strasser, {Erwin F.} and Simon Swift and Hidetoshi Tahara and Muneesh Tewari and Kate Timms and Swasti Tiwari and Rochelle Tixeira and Mercedes Tkach and Toh, {Wei Seong} and Richard Tomasini and Torrecilhas, {Ana Claudia} and Tosar, {Juan Pablo} and Vasilis Toxavidis and Lorena Urbanelli and Pieter Vader and {van Balkom}, {Bas W.M.} and {van der Grein}, {Susanne G.} and {Van Deun}, Jan and {van Herwijnen}, {Martijn J.C.} and {Van Keuren-Jensen}, Kendall and {van Niel}, Guillaume and {van Royen}, {Martin E.} and {van Wijnen}, {Andre J.} and Vasconcelos, {M. Helena} and Vechetti, {Ivan J.} and Veit, {Tiago D.} and Vella, {Laura J.} and {\'E}milie Velot and Verweij, {Frederik J.} and Beate Vestad and Vi{\~n}as, {Jose L.} and Tam{\'a}s Visnovitz and Vukman, {Krisztina V.} and Jessica Wahlgren and Watson, {Dionysios C.} and Wauben, {Marca H.M.} and Alissa Weaver and Webber, {Jason P.} and Viktoria Weber and Wehman, {Ann M.} and Weiss, {Daniel J.} and Welsh, {Joshua A.} and Sebastian Wendt and Wheelock, {Asa M.} and Zolt{\'a}n Wiener and Leonie Witte and Joy Wolfram and Angeliki Xagorari and Patricia Xander and Jing Xu and Xiaomei Yan and Mar{\'i}a Y{\'a}{\~n}ez-M{\'o} and Hang Yin and Yuana Yuana and Valentina Zappulli and Jana Zarubova and Vytautas Žėkas and Zhang, {Jian ye} and Zezhou Zhao and Lei Zheng and Zheutlin, {Alexander R.} and Zickler, {Antje M.} and Pascale Zimmermann and Zivkovic, {Angela M.} and Davide Zocco and Zuba-Surma, {Ewa K.}",
year = "2018",
month = "1",
day = "1",
doi = "10.1080/20013078.2018.1535750",
language = "English (US)",
volume = "7",
journal = "Journal of Extracellular Vesicles",
issn = "2001-3078",
publisher = "Taylor and Francis Ltd.",
number = "1",

}

TY - JOUR

T1 - Minimal information for studies of extracellular vesicles 2018 (MISEV2018)

T2 - a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

AU - Théry, Clotilde

AU - Witwer, Kenneth W.

AU - Aikawa, Elena

AU - Alcaraz, Maria Jose

AU - Anderson, Johnathon D.

AU - Andriantsitohaina, Ramaroson

AU - Antoniou, Anna

AU - Arab, Tanina

AU - Archer, Fabienne

AU - Atkin-Smith, Georgia K.

AU - Ayre, D. Craig

AU - Bach, Jean Marie

AU - Bachurski, Daniel

AU - Baharvand, Hossein

AU - Balaj, Leonora

AU - Baldacchino, Shawn

AU - Bauer, Natalie N.

AU - Baxter, Amy A.

AU - Bebawy, Mary

AU - Beckham, Carla

AU - Bedina Zavec, Apolonija

AU - Benmoussa, Abderrahim

AU - Berardi, Anna C.

AU - Bergese, Paolo

AU - Bielska, Ewa

AU - Blenkiron, Cherie

AU - Bobis-Wozowicz, Sylwia

AU - Boilard, Eric

AU - Boireau, Wilfrid

AU - Bongiovanni, Antonella

AU - Borràs, Francesc E.

AU - Bosch, Steffi

AU - Boulanger, Chantal M.

AU - Breakefield, Xandra

AU - Breglio, Andrew M.

AU - Brennan, Meadhbh

AU - Brigstock, David R.

AU - Brisson, Alain

AU - Broekman, Marike L.D.

AU - Bromberg, Jacqueline F.

AU - Bryl-Górecka, Paulina

AU - Buch, Shilpa J

AU - Buck, Amy H.

AU - Burger, Dylan

AU - Busatto, Sara

AU - Buschmann, Dominik

AU - Bussolati, Benedetta

AU - Buzás, Edit I.

AU - Byrd, James Bryan

AU - Camussi, Giovanni

AU - Carter, David R.F.

AU - Caruso, Sarah

AU - Chamley, Lawrence W.

AU - Chang, Yu Ting

AU - Chaudhuri, Amrita Datta

AU - Chen, Chihchen

AU - Chen, Shuai

AU - Cheng, Lesley

AU - Chin, Andrew R.

AU - Clayton, Aled

AU - Clerici, Stefano P.

AU - Cocks, Alex

AU - Cocucci, Emanuele

AU - Coffey, Robert J.

AU - Cordeiro-da-Silva, Anabela

AU - Couch, Yvonne

AU - Coumans, Frank A.W.

AU - Coyle, Beth

AU - Crescitelli, Rossella

AU - Criado, Miria Ferreira

AU - D’Souza-Schorey, Crislyn

AU - Das, Saumya

AU - de Candia, Paola

AU - De Santana, Eliezer F.

AU - De Wever, Olivier

AU - del Portillo, Hernando A.

AU - Demaret, Tanguy

AU - Deville, Sarah

AU - Devitt, Andrew

AU - Dhondt, Bert

AU - Di Vizio, Dolores

AU - Dieterich, Lothar C.

AU - Dolo, Vincenza

AU - Dominguez Rubio, Ana Paula

AU - Dominici, Massimo

AU - Dourado, Mauricio R.

AU - Driedonks, Tom A.P.

AU - Duarte, Filipe V.

AU - Duncan, Heather M.

AU - Eichenberger, Ramon M.

AU - Ekström, Karin

AU - EL Andaloussi, Samir

AU - Elie-Caille, Celine

AU - Erdbrügger, Uta

AU - Falcón-Pérez, Juan M.

AU - Fatima, Farah

AU - Fish, Jason E.

AU - Flores-Bellver, Miguel

AU - Försönits, András

AU - Frelet-Barrand, Annie

AU - Fricke, Fabia

AU - Fuhrmann, Gregor

AU - Gabrielsson, Susanne

AU - Gámez-Valero, Ana

AU - Gardiner, Chris

AU - Gärtner, Kathrin

AU - Gaudin, Raphael

AU - Gho, Yong Song

AU - Giebel, Bernd

AU - Gilbert, Caroline

AU - Gimona, Mario

AU - Giusti, Ilaria

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PY - 2018/1/1

Y1 - 2018/1/1

N2 - The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

AB - The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

KW - ectosomes

KW - exosomes

KW - extracellular vesicles

KW - guidelines

KW - microparticles

KW - microvesicles

KW - minimal information requirements

KW - reproducibility

KW - rigor

KW - standardization

UR - http://www.scopus.com/inward/record.url?scp=85057143501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057143501&partnerID=8YFLogxK

U2 - 10.1080/20013078.2018.1535750

DO - 10.1080/20013078.2018.1535750

M3 - Article

VL - 7

JO - Journal of Extracellular Vesicles

JF - Journal of Extracellular Vesicles

SN - 2001-3078

IS - 1

M1 - 1535750

ER -