Migration of bovine bronchial epithelial cells to extracellular matrix components.

K. A. Rickard, J. Taylor, Stephen Israel Rennard, J. R. Spurzem

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Migration of epithelial cells is an important feature of wound healing. Components of extracellular matrix stimulate migration of other cells, and we hypothesized that basement membrane components, laminin and type IV collagen, stimulate migration of bovine bronchial epithelial cells (BBEC). BBEC cultured for 3 days were used in migration assays using the blindwell chamber technique. BBEC migrated in a concentration-dependent manner to laminin, type IV collagen, and fibronectin. "Checkerboard" analysis demonstrated that the migration was directional for each of the components studied. We also evaluated the stimulatory activity of fibronectin fragments. A fragment containing the RGDS peptide was stimulatory of migration while other fragments were much less so, suggesting a role for RGDS-sensitive, integrin-mediated mechanisms. In order to evaluate whether bound or soluble matrix components were required to direct cell migration, we performed haptotaxis assays with precoated filters in the migration chambers. Fibronectin-precoated filters demonstrated significant stimulation of migration, suggesting that some of the migration in our original assays with fibronectin present in the lower chambers could be termed haptotactic. laminin- and type IV collagen-precoated filters were less active. When insulin was used as a chemoattractant, the fibronectin-precoated filters were more facilitatory of migration than the other matrix coatings. In summary, BBEC can chemotactically migrate to extracellular matrix components. Thus, the composition of the provisional matrix that forms at sites of epithelial injury may play an important role in the repair processes that occur after injury to the bronchial epithelium.

Original languageEnglish (US)
Pages (from-to)63-68
Number of pages6
JournalAmerican journal of respiratory cell and molecular biology
Volume8
Issue number1
DOIs
StatePublished - Jan 1 1993

Fingerprint

Fibronectins
Extracellular Matrix
Epithelial Cells
Collagen Type IV
Laminin
Assays
Cell Movement
arginyl-glycyl-aspartyl-serine
Chemotactic Factors
Wounds and Injuries
Chemotaxis
Basement Membrane
Integrins
Wound Healing
Repair
Epithelium
Cells
Insulin
Coatings
Chemical analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Migration of bovine bronchial epithelial cells to extracellular matrix components. / Rickard, K. A.; Taylor, J.; Rennard, Stephen Israel; Spurzem, J. R.

In: American journal of respiratory cell and molecular biology, Vol. 8, No. 1, 01.01.1993, p. 63-68.

Research output: Contribution to journalArticle

Rickard, K. A. ; Taylor, J. ; Rennard, Stephen Israel ; Spurzem, J. R. / Migration of bovine bronchial epithelial cells to extracellular matrix components. In: American journal of respiratory cell and molecular biology. 1993 ; Vol. 8, No. 1. pp. 63-68.
@article{7e829d58b8fb43929f5b1127825e7a2e,
title = "Migration of bovine bronchial epithelial cells to extracellular matrix components.",
abstract = "Migration of epithelial cells is an important feature of wound healing. Components of extracellular matrix stimulate migration of other cells, and we hypothesized that basement membrane components, laminin and type IV collagen, stimulate migration of bovine bronchial epithelial cells (BBEC). BBEC cultured for 3 days were used in migration assays using the blindwell chamber technique. BBEC migrated in a concentration-dependent manner to laminin, type IV collagen, and fibronectin. {"}Checkerboard{"} analysis demonstrated that the migration was directional for each of the components studied. We also evaluated the stimulatory activity of fibronectin fragments. A fragment containing the RGDS peptide was stimulatory of migration while other fragments were much less so, suggesting a role for RGDS-sensitive, integrin-mediated mechanisms. In order to evaluate whether bound or soluble matrix components were required to direct cell migration, we performed haptotaxis assays with precoated filters in the migration chambers. Fibronectin-precoated filters demonstrated significant stimulation of migration, suggesting that some of the migration in our original assays with fibronectin present in the lower chambers could be termed haptotactic. laminin- and type IV collagen-precoated filters were less active. When insulin was used as a chemoattractant, the fibronectin-precoated filters were more facilitatory of migration than the other matrix coatings. In summary, BBEC can chemotactically migrate to extracellular matrix components. Thus, the composition of the provisional matrix that forms at sites of epithelial injury may play an important role in the repair processes that occur after injury to the bronchial epithelium.",
author = "Rickard, {K. A.} and J. Taylor and Rennard, {Stephen Israel} and Spurzem, {J. R.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1165/ajrcmb/8.1.63",
language = "English (US)",
volume = "8",
pages = "63--68",
journal = "American Journal of Respiratory Cell and Molecular Biology",
issn = "1044-1549",
publisher = "American Thoracic Society",
number = "1",

}

TY - JOUR

T1 - Migration of bovine bronchial epithelial cells to extracellular matrix components.

AU - Rickard, K. A.

AU - Taylor, J.

AU - Rennard, Stephen Israel

AU - Spurzem, J. R.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Migration of epithelial cells is an important feature of wound healing. Components of extracellular matrix stimulate migration of other cells, and we hypothesized that basement membrane components, laminin and type IV collagen, stimulate migration of bovine bronchial epithelial cells (BBEC). BBEC cultured for 3 days were used in migration assays using the blindwell chamber technique. BBEC migrated in a concentration-dependent manner to laminin, type IV collagen, and fibronectin. "Checkerboard" analysis demonstrated that the migration was directional for each of the components studied. We also evaluated the stimulatory activity of fibronectin fragments. A fragment containing the RGDS peptide was stimulatory of migration while other fragments were much less so, suggesting a role for RGDS-sensitive, integrin-mediated mechanisms. In order to evaluate whether bound or soluble matrix components were required to direct cell migration, we performed haptotaxis assays with precoated filters in the migration chambers. Fibronectin-precoated filters demonstrated significant stimulation of migration, suggesting that some of the migration in our original assays with fibronectin present in the lower chambers could be termed haptotactic. laminin- and type IV collagen-precoated filters were less active. When insulin was used as a chemoattractant, the fibronectin-precoated filters were more facilitatory of migration than the other matrix coatings. In summary, BBEC can chemotactically migrate to extracellular matrix components. Thus, the composition of the provisional matrix that forms at sites of epithelial injury may play an important role in the repair processes that occur after injury to the bronchial epithelium.

AB - Migration of epithelial cells is an important feature of wound healing. Components of extracellular matrix stimulate migration of other cells, and we hypothesized that basement membrane components, laminin and type IV collagen, stimulate migration of bovine bronchial epithelial cells (BBEC). BBEC cultured for 3 days were used in migration assays using the blindwell chamber technique. BBEC migrated in a concentration-dependent manner to laminin, type IV collagen, and fibronectin. "Checkerboard" analysis demonstrated that the migration was directional for each of the components studied. We also evaluated the stimulatory activity of fibronectin fragments. A fragment containing the RGDS peptide was stimulatory of migration while other fragments were much less so, suggesting a role for RGDS-sensitive, integrin-mediated mechanisms. In order to evaluate whether bound or soluble matrix components were required to direct cell migration, we performed haptotaxis assays with precoated filters in the migration chambers. Fibronectin-precoated filters demonstrated significant stimulation of migration, suggesting that some of the migration in our original assays with fibronectin present in the lower chambers could be termed haptotactic. laminin- and type IV collagen-precoated filters were less active. When insulin was used as a chemoattractant, the fibronectin-precoated filters were more facilitatory of migration than the other matrix coatings. In summary, BBEC can chemotactically migrate to extracellular matrix components. Thus, the composition of the provisional matrix that forms at sites of epithelial injury may play an important role in the repair processes that occur after injury to the bronchial epithelium.

UR - http://www.scopus.com/inward/record.url?scp=0027355855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027355855&partnerID=8YFLogxK

U2 - 10.1165/ajrcmb/8.1.63

DO - 10.1165/ajrcmb/8.1.63

M3 - Article

VL - 8

SP - 63

EP - 68

JO - American Journal of Respiratory Cell and Molecular Biology

JF - American Journal of Respiratory Cell and Molecular Biology

SN - 1044-1549

IS - 1

ER -