Microvascular alterations develop in Syrian hamsters after the induction of diabetes mellitus by streptozotocin

W. L. Joyner, W. G. Mayhan, R. L. Johnson, C. K. Phares

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Microvessels in the cheek pouch of the hamster were investigated to determine their structural, reactivity, and permeability characteristics after the induction of diabetes. To induce diabetes, hamsters were injected with streptozotocin (50 mg/kg body wt/day, i.p., for 3 days). Vehicle-injected, age-matched hamsters were the controls. Diabetic hamsters were characterized by elevated serum glucose (>300 mg/dl) and triglycerides and decreased serum insulin (50%). Microvascular studies were completed on cheek pouch microvessels suffused with Ringer's solution (37°C, pH 7.4) bubbled with 95% N2-5% CO2. Vascular dimensions and reactivity of selected arterioles and venules to microapplications of norepinephrine were determined with a video micrometer using intravital microscopy. Restrictiveness of the microvascular membranes to fluorescein-labeled dextran fractions (mol wt: 150,000; 40,000; 20,000 daltons) was measured by determining the number of leaky sites. Stimulation of membrane permeability by histamine was investigated. There were no major alterations in arteriolar lumen and wall diameters, whereas venular lumen diameters were increased in hamsters diabetic for two months. Likewise, arteriolar responses to norepinephrine were not altered by diabetes; however, venular responses were decreased at two months. The restrictiveness of the vascular membrane to various dextran fractions was dramatically decreased in the diabetic animals at two months. Histamine did not alter microvascular leakage in the diabetic as it did in the normal hamsters. These studies indicate that microvascular alterations, venular dilation, and increased permeability to large molecules occur in the diabetic hamster within two months after the induction of diabetes.

Original languageEnglish (US)
Pages (from-to)93-100
Number of pages8
JournalDiabetes
Volume30
Issue number2
DOIs
StatePublished - Jan 1 1981

Fingerprint

Mesocricetus
Streptozocin
Cricetinae
Diabetes Mellitus
Permeability
Cheek
Microvessels
Histamine
Membranes
Blood Vessels
Norepinephrine
Venules
Arterioles
Dextrans
Serum
Dilatation
Triglycerides
Insulin
Glucose

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Microvascular alterations develop in Syrian hamsters after the induction of diabetes mellitus by streptozotocin. / Joyner, W. L.; Mayhan, W. G.; Johnson, R. L.; Phares, C. K.

In: Diabetes, Vol. 30, No. 2, 01.01.1981, p. 93-100.

Research output: Contribution to journalArticle

Joyner, W. L. ; Mayhan, W. G. ; Johnson, R. L. ; Phares, C. K. / Microvascular alterations develop in Syrian hamsters after the induction of diabetes mellitus by streptozotocin. In: Diabetes. 1981 ; Vol. 30, No. 2. pp. 93-100.
@article{615955ef5587429896109599638d6f80,
title = "Microvascular alterations develop in Syrian hamsters after the induction of diabetes mellitus by streptozotocin",
abstract = "Microvessels in the cheek pouch of the hamster were investigated to determine their structural, reactivity, and permeability characteristics after the induction of diabetes. To induce diabetes, hamsters were injected with streptozotocin (50 mg/kg body wt/day, i.p., for 3 days). Vehicle-injected, age-matched hamsters were the controls. Diabetic hamsters were characterized by elevated serum glucose (>300 mg/dl) and triglycerides and decreased serum insulin (50{\%}). Microvascular studies were completed on cheek pouch microvessels suffused with Ringer's solution (37°C, pH 7.4) bubbled with 95{\%} N2-5{\%} CO2. Vascular dimensions and reactivity of selected arterioles and venules to microapplications of norepinephrine were determined with a video micrometer using intravital microscopy. Restrictiveness of the microvascular membranes to fluorescein-labeled dextran fractions (mol wt: 150,000; 40,000; 20,000 daltons) was measured by determining the number of leaky sites. Stimulation of membrane permeability by histamine was investigated. There were no major alterations in arteriolar lumen and wall diameters, whereas venular lumen diameters were increased in hamsters diabetic for two months. Likewise, arteriolar responses to norepinephrine were not altered by diabetes; however, venular responses were decreased at two months. The restrictiveness of the vascular membrane to various dextran fractions was dramatically decreased in the diabetic animals at two months. Histamine did not alter microvascular leakage in the diabetic as it did in the normal hamsters. These studies indicate that microvascular alterations, venular dilation, and increased permeability to large molecules occur in the diabetic hamster within two months after the induction of diabetes.",
author = "Joyner, {W. L.} and Mayhan, {W. G.} and Johnson, {R. L.} and Phares, {C. K.}",
year = "1981",
month = "1",
day = "1",
doi = "10.2337/diab.30.2.93",
language = "English (US)",
volume = "30",
pages = "93--100",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association Inc.",
number = "2",

}

TY - JOUR

T1 - Microvascular alterations develop in Syrian hamsters after the induction of diabetes mellitus by streptozotocin

AU - Joyner, W. L.

AU - Mayhan, W. G.

AU - Johnson, R. L.

AU - Phares, C. K.

PY - 1981/1/1

Y1 - 1981/1/1

N2 - Microvessels in the cheek pouch of the hamster were investigated to determine their structural, reactivity, and permeability characteristics after the induction of diabetes. To induce diabetes, hamsters were injected with streptozotocin (50 mg/kg body wt/day, i.p., for 3 days). Vehicle-injected, age-matched hamsters were the controls. Diabetic hamsters were characterized by elevated serum glucose (>300 mg/dl) and triglycerides and decreased serum insulin (50%). Microvascular studies were completed on cheek pouch microvessels suffused with Ringer's solution (37°C, pH 7.4) bubbled with 95% N2-5% CO2. Vascular dimensions and reactivity of selected arterioles and venules to microapplications of norepinephrine were determined with a video micrometer using intravital microscopy. Restrictiveness of the microvascular membranes to fluorescein-labeled dextran fractions (mol wt: 150,000; 40,000; 20,000 daltons) was measured by determining the number of leaky sites. Stimulation of membrane permeability by histamine was investigated. There were no major alterations in arteriolar lumen and wall diameters, whereas venular lumen diameters were increased in hamsters diabetic for two months. Likewise, arteriolar responses to norepinephrine were not altered by diabetes; however, venular responses were decreased at two months. The restrictiveness of the vascular membrane to various dextran fractions was dramatically decreased in the diabetic animals at two months. Histamine did not alter microvascular leakage in the diabetic as it did in the normal hamsters. These studies indicate that microvascular alterations, venular dilation, and increased permeability to large molecules occur in the diabetic hamster within two months after the induction of diabetes.

AB - Microvessels in the cheek pouch of the hamster were investigated to determine their structural, reactivity, and permeability characteristics after the induction of diabetes. To induce diabetes, hamsters were injected with streptozotocin (50 mg/kg body wt/day, i.p., for 3 days). Vehicle-injected, age-matched hamsters were the controls. Diabetic hamsters were characterized by elevated serum glucose (>300 mg/dl) and triglycerides and decreased serum insulin (50%). Microvascular studies were completed on cheek pouch microvessels suffused with Ringer's solution (37°C, pH 7.4) bubbled with 95% N2-5% CO2. Vascular dimensions and reactivity of selected arterioles and venules to microapplications of norepinephrine were determined with a video micrometer using intravital microscopy. Restrictiveness of the microvascular membranes to fluorescein-labeled dextran fractions (mol wt: 150,000; 40,000; 20,000 daltons) was measured by determining the number of leaky sites. Stimulation of membrane permeability by histamine was investigated. There were no major alterations in arteriolar lumen and wall diameters, whereas venular lumen diameters were increased in hamsters diabetic for two months. Likewise, arteriolar responses to norepinephrine were not altered by diabetes; however, venular responses were decreased at two months. The restrictiveness of the vascular membrane to various dextran fractions was dramatically decreased in the diabetic animals at two months. Histamine did not alter microvascular leakage in the diabetic as it did in the normal hamsters. These studies indicate that microvascular alterations, venular dilation, and increased permeability to large molecules occur in the diabetic hamster within two months after the induction of diabetes.

UR - http://www.scopus.com/inward/record.url?scp=0019405885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019405885&partnerID=8YFLogxK

U2 - 10.2337/diab.30.2.93

DO - 10.2337/diab.30.2.93

M3 - Article

C2 - 6162695

AN - SCOPUS:0019405885

VL - 30

SP - 93

EP - 100

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 2

ER -