Microstructural changes to proso millet protein bodies upon cooking and digestion

Paridhi Gulati, You Zhou, Christian Elowsky, Devin J Rose

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cooking results in a drastic decline in digestibility of proso millet proteins, panicins. Scanning electron and confocal microscopy were used to observe morphological changes in proso millet protein bodies upon cooking and digestion that could be associated with the loss in digestibility. Spherical protein bodies (1–2.5 μm) were observed in proso millet flour and extracted protein. Cooking did not result in any noticeable change in the size or shape of the protein bodies. However, upon digestion with pepsin the poor digestibility of cooked proso millet protein was clearly evident from the differences in microstructure of the protein bodies: large cavities were observed in the uncooked protein bodies while cooked protein bodies had only tiny holes. When proso millet was cooked in 8 M urea and then digested, the protein bodies appeared similar to uncooked digested protein bodies. The morphological changes observed in proso millet protein upon cooking and digestion did not show any visible aggregates, but the inability of pepsin to digest cooked protein bodies was clearly evident under microscopy and is in agreement with the chemical analyses reported previously.

Original languageEnglish (US)
Pages (from-to)80-86
Number of pages7
JournalJournal of Cereal Science
Volume80
DOIs
StatePublished - Mar 1 2018

Fingerprint

Panicum miliaceum subsp. miliaceum
Panicum
protein bodies
Cooking
cooking
Digestion
digestion
Proteins
digestibility
pepsin
Pepsin A
proteins
millet flour
protein aggregates
microstructure
microscopy
scanning electron microscopy
Confocal microscopy
urea
Flour

Keywords

  • Confocal microscopy
  • Enzymatic hydrolysis
  • Scanning electron microscopy
  • Wet milling

ASJC Scopus subject areas

  • Food Science
  • Biochemistry

Cite this

Microstructural changes to proso millet protein bodies upon cooking and digestion. / Gulati, Paridhi; Zhou, You; Elowsky, Christian; Rose, Devin J.

In: Journal of Cereal Science, Vol. 80, 01.03.2018, p. 80-86.

Research output: Contribution to journalArticle

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