Microrna expression and regulation in human ovarian carcinoma cells by luteinizing hormone

Juan Cui, Joanna B. Eldredge, Ying Xu, David Puett

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: MicroRNAs have been widely-studied with regard to their aberrant expression and high correlation with tumorigenesis and progression in various solid tumors. With the major goal of assessing gonadotropin (luteinizing hormone, LH) contributions to LH receptor (LHR)-positive ovarian cancer cells, we have conducted a genome-wide transcriptomic analysis on human epithelial ovarian cancer cells to identify the microRNA-associated cellular response to LH-mediated activation of LHR. Methods: Human ovarian cancer cells (SKOV3) were chosen as negative control (LHR-) and stably transfected to express functional LHR (LHR+), followed by incubation with LH (0-20 h). At different times of LH-mediated activation of LHR the cancer cells were analyzed by a high-density Ovarian Cancer Disease-Specific-Array (DSA, ALMAC™), which profiled ~100,000 transcripts with ~400 non-coding microRNAs. Findings: In total, 65 microRNAs were identified to exhibit differential expression in either LHR expressing SKOV3 cells or LH-treated cells, a few of which have been found in the genomic fragile regions that are associated with abnormal deletion or amplification in cancer, such as miR-21, miR-101-1, miR-210 and miR-301a. By incorporating the dramatic expression changes observed in mRNAs, strong microRNA/mRNA regulatory pairs were predicted through statistical analyses coupled with collective computational prediction. The role of each microRNA was then determined through a functional analysis based on the highly-confident microRNA/mRNA pairs. Conclusion: The overall impact on the transcriptome-level expression indicates that LH may regulate apoptosis and cell growth of LHR+ SKOV3 cells, particularly by reducing cancer cell proliferation, with some microRNAs involved in regulatory roles.

Original languageEnglish (US)
Article numbere21730
JournalPloS one
Volume6
Issue number7
DOIs
StatePublished - Jul 15 2011

Fingerprint

LH Receptors
luteinizing hormone
Luteinizing Hormone
MicroRNAs
microRNA
carcinoma
Cells
Carcinoma
ovarian neoplasms
receptors
cells
Ovarian Neoplasms
Messenger RNA
neoplasms
Neoplasms
Chemical activation
Ovarian Diseases
Functional analysis
Cell proliferation
Cell growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Microrna expression and regulation in human ovarian carcinoma cells by luteinizing hormone. / Cui, Juan; Eldredge, Joanna B.; Xu, Ying; Puett, David.

In: PloS one, Vol. 6, No. 7, e21730, 15.07.2011.

Research output: Contribution to journalArticle

Cui, Juan ; Eldredge, Joanna B. ; Xu, Ying ; Puett, David. / Microrna expression and regulation in human ovarian carcinoma cells by luteinizing hormone. In: PloS one. 2011 ; Vol. 6, No. 7.
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