MicroRNA-146a modulates human bronchial epithelial cell survival in response to the cytokine-induced apoptosis

Xiang-de Liu, Amy Nelson, Xingqi Wang, Nobuhiro Kanaji, Miok Kim, Tadashi Sato, Masanori Nakanishi, YingJi Li, Jianhong Sun, Joel Michalski, Amol N Patil, Hesham E Basma, Stephen I. Rennard

Research output: Contribution to journalArticle

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Abstract

MicroRNA plays an important role in cell differentiation, proliferation and cell death. The current study found that miRNA-146a was up-regulated in human bronchial epithelial cells (HBECs) in response to stimulation by TGF-ß1 plus cytomix (a mixture of IL-1ß, IFN-γ and TNF-α). TGF-ß1 plus cytomix (TCM) induced apoptosis in HBECs (3.4 ± 0.6% of control vs 83.1 ± 4.0% of TCM treated cells, p < 0.01), and this was significantly blocked by the miRNA-146a mimic (8.8 ± 1.5%, p < 0.01). In contrast, a miRNA-146a inhibitor had only a modest effect on cell survival but appeared to augment the induction of epithelial-mesenchymal transition (EMT) in response to the cytokines. The MicroRNA-146a mimic appears to modulate HBEC survival through a mechanism of up-regulating Bcl-XL and STAT3 phosphorylation, and by this mechanism it could contribute to tissue repair and remodeling.

Original languageEnglish (US)
Pages (from-to)177-182
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume380
Issue number1
DOIs
StatePublished - Feb 27 2009

Fingerprint

MicroRNAs
Cell Survival
Epithelial Cells
Apoptosis
Cytokines
Cells
Phosphorylation
Epithelial-Mesenchymal Transition
Cell death
Interleukin-1
Cell Differentiation
Repair
Cell Death
Cell Proliferation
human MIRN146 microRNA
Tissue

Keywords

  • Apoptosis
  • Bronchial epithelial cell
  • Survival
  • miRNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

MicroRNA-146a modulates human bronchial epithelial cell survival in response to the cytokine-induced apoptosis. / Liu, Xiang-de; Nelson, Amy; Wang, Xingqi; Kanaji, Nobuhiro; Kim, Miok; Sato, Tadashi; Nakanishi, Masanori; Li, YingJi; Sun, Jianhong; Michalski, Joel; Patil, Amol N; Basma, Hesham E; Rennard, Stephen I.

In: Biochemical and Biophysical Research Communications, Vol. 380, No. 1, 27.02.2009, p. 177-182.

Research output: Contribution to journalArticle

Liu, Xiang-de ; Nelson, Amy ; Wang, Xingqi ; Kanaji, Nobuhiro ; Kim, Miok ; Sato, Tadashi ; Nakanishi, Masanori ; Li, YingJi ; Sun, Jianhong ; Michalski, Joel ; Patil, Amol N ; Basma, Hesham E ; Rennard, Stephen I. / MicroRNA-146a modulates human bronchial epithelial cell survival in response to the cytokine-induced apoptosis. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 380, No. 1. pp. 177-182.
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