MicroRNA-146a modulates human bronchial epithelial cell survival in response to the cytokine-induced apoptosis

Xiang-de Liu, Amy Nelson, Xingqi Wang, Nobuhiro Kanaji, Miok Kim, Tadashi Sato, Masanori Nakanishi, YingJi Li, Jianhong Sun, Joel Michalski, Amol N Patil, Hesham E Basma, Stephen I. Rennard

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

MicroRNA plays an important role in cell differentiation, proliferation and cell death. The current study found that miRNA-146a was up-regulated in human bronchial epithelial cells (HBECs) in response to stimulation by TGF-ß1 plus cytomix (a mixture of IL-1ß, IFN-γ and TNF-α). TGF-ß1 plus cytomix (TCM) induced apoptosis in HBECs (3.4 ± 0.6% of control vs 83.1 ± 4.0% of TCM treated cells, p < 0.01), and this was significantly blocked by the miRNA-146a mimic (8.8 ± 1.5%, p < 0.01). In contrast, a miRNA-146a inhibitor had only a modest effect on cell survival but appeared to augment the induction of epithelial-mesenchymal transition (EMT) in response to the cytokines. The MicroRNA-146a mimic appears to modulate HBEC survival through a mechanism of up-regulating Bcl-XL and STAT3 phosphorylation, and by this mechanism it could contribute to tissue repair and remodeling.

Original languageEnglish (US)
Pages (from-to)177-182
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume380
Issue number1
DOIs
Publication statusPublished - Feb 27 2009

    Fingerprint

Keywords

  • Apoptosis
  • Bronchial epithelial cell
  • Survival
  • miRNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this